The enzyme from Solanum lycopersicum has very poor affinity with geranyl diphosphate as substrate. Catalyses the formation of the acyclic myrcene and ocimene as major products in addition to β-phellandrene .
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The enzyme from Solanum lycopersicum has very poor affinity with geranyl diphosphate as substrate. Catalyses the formation of the acyclic myrcene and ocimene as major products in addition to beta-phellandrene [1].
Substrates: - Products: main product is beta-phellandrene. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glandular trichome. In tomato glands, neryl diphosphate serves as a precursor for the synthesis of monoterpenes
Substrates: neryl diphosphate most likely ionizes to a neryl cation, which can further isomerize to an alpha-terpinyl cation. Further transformations of this cyclic intermediate can eventually yield all observed products of PHS1 Products: the main product is beta-phellandrene
Substrates: - Products: main product is beta-phellandrene. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glandular trichome. In tomato glands, neryl diphosphate serves as a precursor for the synthesis of monoterpenes
transgenic fruits overexpressing isoform PHS1 produce only small amounts of GPP-derived monoterpene products. Neryl diphosphate synthase NDPS1 coexpression with PHS1 results in a monoterpene blend, including beta-phellandrene, similar to that produced from NPP by PHS1 in vitro and in trichomes. Fruits coexpressing both PHS1 and NDPS1 show recovery of lycopene levels compared to NDPS1 fruits. In vitro assays demonstrate that NPP serves as an inhibitor of geranylgeranyl diphosphate synthase. Monoterpenes produced in fruits coexpressing both PHS1 and NDPS1 contribute to direct plant defense negatively affecting feeding behavior of the herbivore Helicoverpa zea and displaying antifungal activity against Botrytis cinerea
the complete ORF of PHS1 encodes a plastid transit peptide, construction of an ORF of PHS1 that begins at Met45 and includes a His-tag extension at the N-terminus, expression in Eschreichia coli
monoterpenes produced in fruits overexpressing both isoform PHS1 and neryl diphsphate synthase NDPS1 contribute to direct plant defense negatively affecting feeding behavior of the herbivore Helicoverpa zea and displaying antifungal activity against Botrytis cinerea
Heterologous leader sequences in fusion constructs enhance expression of geranyl diphosphate synthase and yield of beta-phellandrene production in cyanobacteria (Synechocystis)
Mono- and sesquiterpene release from tomato (Solanum lycopersicum) leaves upon mild and severe heat stress and through recovery From gene expression to emission responses
A phycocyanin-phellandrene synthase fusion enhances recombinant protein expression and beta-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria)
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