We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 4.2.3.153 - (5-formylfuran-3-yl)methyl phosphate synthase for references in articles please use BRENDA:EC4.2.3.153
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme catalyses the reaction in the direction of producing (5-formylfuran-3-yl)methyl phosphate, an intermediate in the biosynthesis of methanofuran. The sequence of events starts with the removal of a phosphate group, followed by aldol condensation and cyclization. Methanofuran is a carbon-carrier cofactor involved in the first step of the methanogenic reduction of carbon dioxide by methanogenic archaea.
The expected taxonomic range for this enzyme is: Methanocaldococcus jannaschii
Synonyms
mj1099, 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-furaldehyde phosphate synthase
4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase
2-furaldehyde phosphate synthase
-
2-furaldehyde phosphate synthase
-
-
4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase
-
4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase
-
-
4-HFC-P synthase
-
mfnB
-
mfnB
; gene name; gene name
-
MJ1099
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 D-glyceraldehyde 3-phosphate = (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
2 D-glyceraldehyde 3-phosphate = (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
-
-
-
-
2 D-glyceraldehyde 3-phosphate = (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
a phosphate elimination reaction and a triose phosphate isomerase-like reaction occur at the glyceraldehyde 3-phosphate binding site I and II, respectively, prior to the aldol condensation between the enzyme-bound enol form of methylglyoxal and dihydroxyacetone phosphate, after which the catalytic cycle is completed by a cyclization and two dehydration reactions assisted by several general acids/bases at the same active site
-
2 D-glyceraldehyde 3-phosphate = (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
a phosphate elimination reaction and a triose phosphate isomerase-like reaction occur at the glyceraldehyde 3-phosphate binding site I and II, respectively, prior to the aldol condensation between the enzyme-bound enol form of methylglyoxal and dihydroxyacetone phosphate, after which the catalytic cycle is completed by a cyclization and two dehydration reactions assisted by several general acids/bases at the same active site
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glyceraldehyde-3-phosphate phosphate-lyase [D-glyceraldehyde-3-phosphate-adding; (5-formylfuran-3-yl)methyl-phosphate-forming]
The enzyme catalyses the reaction in the direction of producing (5-formylfuran-3-yl)methyl phosphate, an intermediate in the biosynthesis of methanofuran. The sequence of events starts with the removal of a phosphate group, followed by aldol condensation and cyclization. Methanofuran is a carbon-carrier cofactor involved in the first step of the methanogenic reduction of carbon dioxide by methanogenic archaea.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 D-glyceraldehyde 3-phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
additional information
?
-
2 D-glyceraldehyde 3-phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
-
-
-
-
?
2 D-glyceraldehyde 3-phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
-
-
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
2 D-glyceraldehyde 3-phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
-
-
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
2 D-glyceraldehyde 3-phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
-
-
-
-
?
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
the enzyme is involved in biosynthesis of methanofuran, a coenzyme involved in the reduction of carbon dioxide to methane
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
the enzyme is not able to use pyruvate as substrate
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
the enzyme is involved in biosynthesis of methanofuran, a coenzyme involved in the reduction of carbon dioxide to methane
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
the enzyme is not able to use pyruvate as substrate
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
additional information
?
-
-
no substrates: pyruvate, methylglyoxal, phosphoenolpyruvate, oxaloacetic acid, dihydroxyacetone, and dihydroxyacetone phosphate
-
-
-
additional information
?
-
-
no substrates: pyruvate, methylglyoxal, phosphoenolpyruvate, oxaloacetic acid, dihydroxyacetone, and dihydroxyacetone phosphate
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
Q58499
the enzyme is involved in biosynthesis of methanofuran, a coenzyme involved in the reduction of carbon dioxide to methane
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
D-glyceraldehyde 3-phosphate + glycerone phosphate
(5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O
Q58499
the enzyme is involved in biosynthesis of methanofuran, a coenzyme involved in the reduction of carbon dioxide to methane
(5-formylfuran-3-yl)methyl phosphate i.e. 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.035 - 0.048
D-glyceraldehyde
0.035
D-glyceraldehyde
-
wild-type, pH 7.0, 70°C
0.048
D-glyceraldehyde
-
mutant K155R, pH 7.0, 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.022 - 0.026
D-glyceraldehyde
0.022
D-glyceraldehyde
-
mutant K155R, pH 7.0, 70°C
0.026
D-glyceraldehyde
-
wild-type, pH 7.0, 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.46 - 0.74
D-glyceraldehyde
0.46
D-glyceraldehyde
-
mutant K155R, pH 7.0, 70°C
0.74
D-glyceraldehyde
-
wild-type, pH 7.0, 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
the enzyme is involved in biosynthesis of methanofuran, a coenzyme involved in the reduction of carbon dioxide to methane
physiological function
-
the enzyme is involved in biosynthesis of methanofuran, a coenzyme involved in the reduction of carbon dioxide to methane
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25000
-
x * 25000, SDS-PAGE
26053
-
6 * 26053, calculated and crystallization data
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 25000, SDS-PAGE
?
-
x * 25000, SDS-PAGE
-
hexamer
-
6 * 26053, calculated and crystallization data
hexamer
-
6 * 26053, calculated and crystallization data
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
molecular docking of glycerinaldehyde 3-phosphate into the active site
-
to 1.7 A resolution. Enzyme is a member of the TIM-barrel superfamily and the biological unit is a homohexamer
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D151N
-
mutation of strictly conserved residue around the active site, complete inactivation
D25N
-
mutation of strictly conserved residue around the active site, complete inactivation
K155R
-
kcat/KM value comparable to the wild-type
K27R
-
mutation of strictly conserved residue around the active site, complete inactivation
K85R
-
mutation of strictly conserved residue around the active site, complete inactivation
D151N
-
mutation of strictly conserved residue around the active site, complete inactivation
-
D25N
-
mutation of strictly conserved residue around the active site, complete inactivation
-
K155R
-
kcat/KM value comparable to the wild-type
-
K27R
-
mutation of strictly conserved residue around the active site, complete inactivation
-
K85R
-
mutation of strictly conserved residue around the active site, complete inactivation
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Miller, D.; Wang, Y.; Xu, H.; Harich, K.; White, R.H.
Biosynthesis of the 5-(aminomethyl)-3-furanmethanol moiety of methanofuran
Biochemistry
53
4635-4647
2014
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii (Q58499), Methanocaldococcus jannaschii DSM 2661 (Q58499)
brenda
Wang, Y.; Xu, H.; Harich, K.C.; White, R.H.
Identification and characterization of a tyramine-glutamate ligase (MfnD) involved in methanofuran biosynthesis
Biochemistry
53
6220-6230
2014
Methanocaldococcus jannaschii (Q58499), Methanocaldococcus jannaschii DSM 2661 (Q58499)
brenda
Bobik, T.A.; Morales, E.J.; Shin, A.; Cascio, D.; Sawaya, M.R.; Arbing, M.; Yeates, T.O.; Rasche, M.E.
Structure of the methanofuran/methanopterin-biosynthetic enzyme MJ1099 from Methanocaldococcus jannaschii
Acta Crystallogr. Sect. F
70
1472-1479
2014
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii (Q58499), Methanocaldococcus jannaschii DSM 2661 (Q58499)
brenda
Wang, Y.; Jones, M.K.; Xu, H.; Ray, W.K.; White, R.H.
Mechanism of the enzymatic synthesis of 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate (4-HFC-P) from glyceraldehyde-3-phosphate catalyzed by 4-HFC-P synthase
Biochemistry
54
2997-3008
2015
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii (Q58499), Methanocaldococcus jannaschii DSM 2661 (Q58499)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
4.2.3.153 (5-formylfuran-3-yl)methyl phosphate synthase
more
top
Information
