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Information on EC 4.1.1.74 - indolepyruvate decarboxylase and Organism(s) Azospirillum brasilense and UniProt Accession P51852

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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.74 indolepyruvate decarboxylase
IUBMB Comments
Thiamine diphosphate- and Mg2+-dependent. More specific than EC 4.1.1.1 pyruvate decarboxylase
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This record set is specific for:
Azospirillum brasilense
UNIPROT: P51852
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Word Map
The taxonomic range for the selected organisms is: Azospirillum brasilense
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
indolepyruvate decarboxylase, indole-3-pyruvate decarboxylase, indole-3-pyruvic acid decarboxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Indole-3-pyruvate decarboxylase
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Decarboxylase, indolepyruvate
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-
-
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Decarboxylase, indolepyruvate (Enterobacter agglomerans clone pMB2 gene ipdC reduced)
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-
-
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GenBank L80006-derived protein GI 1507711
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-
-
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Indole-3-pyruvate decarboxylase
Indolepyruvate decarboxylase
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-
-
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Indolepyruvate decarboxylase (Enterobacter herbocola strain 299R clone pMB2 gene ipdC reduced)
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-
-
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Indolepyruvic acid decarboxylase
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
-
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PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
3-(indol-3-yl)pyruvate carboxy-lyase [(2-indol-3-yl)acetaldehyde-forming]
Thiamine diphosphate- and Mg2+-dependent. More specific than EC 4.1.1.1 pyruvate decarboxylase
CAS REGISTRY NUMBER
COMMENTARY hide
183213-32-3
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9074-92-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
?
3-(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
?
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
3-(Indol-3-yl)pyruvate
Indole-3-acetaldehyde + CO2
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
?
3-(indol-3-yl)pyruvate
(indole-3-yl)acetaldehyde + CO2
show the reaction diagram
-
-
-
?
3-(Indol-3-yl)pyruvate
?
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SMIT568s10
knock-out strain, SMIT568s10 which shows a significant (50%) decrease in the levels of (indole-3-yl)acetic acid, (indole-3-yl)acetaldehyde and tryptophol compared to the wild type strain SM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
indole-3-acetic acid
expression of ipdC is upregulated by indole-3-acetic acid, other auxins, and nutrient stress
L-tryptophan
at low concentrations, Trp does not influence ipdC expression but 2fold induction is observed at 2.5 mM Trp
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.102
strain SMIT568s10, product: (indole-3-yl)ethanol, substrate: -Trp
0.217
strain SMIT568s10, product: (indole-3-yl)acetaldehyde, substrate: -Trp
0.268
strain SM, product: (indole-3-yl)ethanol, substrate: -Trp
0.615
strain SM, product: (indole-3-yl)acetaldehyde , substrate: -Trp
0.626
strain SMIT568s10, product: (indole-3-yl)ethanol, substrate: +Trp
1.006
strain SMIT568s10, product: (indole-3-yl)acetaldehyde, substrate: +Trp
1.252
strain SM, product: (indole-3-yl)ethanol, substrate: +Trp
2.3
strain SM, product: (indole-3-yl)acetaldehyde , substrate: +Trp
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2
external pH influences ipdC expression as maximum expression is observed at the alkaline pH of 8.2 (69% increase) while highly acidic pH decreases the expression level. At pH 4.2, expression is ca. 32% of that observed at pH 7.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
temperatures on either side of the growth optimum (30°C) are found to alter ipdC expression significantly. At 25°C a 48% increase and at 37°C an 86% increase are noted
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DCIP_AZOBR
545
0
57981
Swiss-Prot
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
heterologous overexpression of a functional iaaC gene from strain Sp245 in strain SM confirms its role in controlling IAA biosynthesis and lowering ipdC expression which may be effected by dissociating indole-3-acetic acid-transcriptional regulator interactions in the 5'region
expressed in Pseudomonas fluorescens strain Psd
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zimmer, W.; Wesche, M.; Timmermans, L.
Identification and isolation of the indole-3-pyruvate decarboxylase gene from Azospirillum brasilense Sp7: sequencing and functional analysis of the gene locus
Curr. Microbiol.
36
327-331
1998
Azospirillum brasilense, Azospirillum brasilense Sp7
Manually annotated by BRENDA team
vande Broek, A.; Lambrecht, M.; Eggermont, K.; Vanderleyden, J.
Auxins upregulate expression of the indole-3-pyruvate decarboxylase gene in Azospirillum brasilense
J. Bacteriol.
181
1338-1342
1999
Azospirillum brasilense
Manually annotated by BRENDA team
Malhotra, M.; Srivastava, S.
An ipdC gene knock-out of Azospirillum brasilense strain SM and its implications on indole-3-acetic acid biosynthesis and plant growth promotion
Antonie van Leeuwenhoek
93
425-433
2008
Azospirillum brasilense (P51852), Azospirillum brasilense
Manually annotated by BRENDA team
Malhotra, M.; Srivastava, S.
Organization of the ipdC region regulates IAA levels in different Azospirillum brasilense strains: molecular and functional analysis of ipdC in strain SM
Environ. Microbiol.
10
1365-1373
2008
Azospirillum brasilense (P51852), Azospirillum brasilense
Manually annotated by BRENDA team
Venieraki, A.; Dimou, M.; Pergalis, P.; Kefalogianni, I.; Chatzipavlidis, I.; Katinakis, P.
The genetic diversity of culturable nitrogen-fixing bacteria in the rhizosphere of wheat
Microb. Ecol.
61
277-285
2011
Azospirillum zeae (D6CI72), Azospirillum brasilense (D6CI73)
Manually annotated by BRENDA team
Kochar, M.; Upadhyay, A.; Srivastava, S.
Indole-3-acetic acid biosynthesis in the biocontrol strain Pseudomonas fluorescens Psd and plant growth regulation by hormone overexpression
Res. Microbiol.
162
426-435
2011
Azospirillum brasilense, Azospirillum brasilense SM, no activity in Pseudomonas fluorescens, no activity in Pseudomonas fluorescens Psd
Manually annotated by BRENDA team