Information on EC 4.1.1.63 - protocatechuate decarboxylase

for references in articles please use BRENDA:EC4.1.1.63
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
4.1.1.63
-
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RECOMMENDED NAME
GeneOntology No.
protocatechuate decarboxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3,4-dihydroxybenzoate = catechol + CO2
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
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-
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SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxybenzoate carboxy-lyase (catechol-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37290-55-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Aerobacter aerogenes
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Clostridium hydroxybenzoicum, JW/Z-1T
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-Dihydroxybenzoate
Catechol + CO2
show the reaction diagram
Catechol + CO2
3,4-Dihydroxybenzoate
show the reaction diagram
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-
-
-
catechol + KHCO3
3,4-dihydroxybenzoate
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3,4-Trihydroxybenzoate
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-
3,4,5-Trihydroxybenzoate
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-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.59
3,4-dihydroxybenzoate
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-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
90
3,4-dihydroxybenzoate
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
under anaerobic conditions, both directions
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
-
5 * 57000, SDS-PAGE
270000
-
non-denaturing gradient PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentamer
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5 * 57000, SDS-PAGE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.5
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25°C, 2 h, stable
4747
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11 - 50
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pH 7.0, relatively stable
25
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pH 6.5-9.5, 2 h, stable
55
-
half-life: 1.5 h
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of 5 mM dithiothreitol and 20 mM Na2S2O3 to a cell-free extract stabilizes the enzyme
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen sensitive. 50% loss of activity after 10 min at 25°C, when exposed to air
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4747
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, cell-free extract in the absence of sulfhydryl-protecting reagents, 2 h, 50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
3,4-dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 is induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium
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LINKS TO OTHER DATABASES (specific for EC-Number 4.1.1.63)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)