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Information on EC 3.6.1.71 - adenosine-5'-diphospho-5'-[DNA] diphosphatase and Organism(s) Mus musculus and UniProt Accession Q7TQC5

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IUBMB Comments
Aprataxin is a DNA-binding protein involved in different types of DNA break repair. The enzyme acts (among other activities) on abortive DNA ligation intermediates that contain an adenylate covalently linked to the 5'-phosphate DNA terminus. It also acts when the adenylate is covalently linked to the 5'-phosphate of a ribonucleotide linked to a DNA strand, which is the result of abortive ligase activty on products of EC 3.1.26.4, ribonuclease H, an enzyme that cleaves RNA-DNA hybrids on the 5' side of the ribonucleotide found in the 5'-RNA-DNA-3' junction. Aprataxin binds the adenylate group to a histidine residue within the active site, followed by its hydrolysis from the nucleic acid and eventual release, leaving a 5'-phosphate terminus that can be efficiently rejoined. The enzyme also possesses the activities of EC 3.6.1.70, guanosine-5'-diphospho-5'-[DNA] diphosphatase, and EC 3.6.1.72, DNA-3'-diphospho-5'-guanosine diphosphatase.
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This record set is specific for:
Mus musculus
UNIPROT: Q7TQC5
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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adenosine-5'-diphospho-5'-[DNA]
+
H2O
=
AMP
+
phospho-5'-[DNA]
adenosine-5'-diphospho-5'-[DNA]
+
=
+
phospho-5'-[DNA]
adenosine-5'-diphospho-5'-(ribonucleotide)-[DNA]
+
H2O
=
AMP
+
5'-phospho-(ribonucleotide)-[DNA]
adenosine-5'-diphospho-5'-(ribonucleotide)-[DNA]
+
=
+
5'-phospho-(ribonucleotide)-[DNA]
Synonyms
aprataxin, dna-adenylate hydrolase, rna-dna deadenylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5'-App5'-DNA adenylate hydrolase
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aprataxin
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APTX
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HNT3
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SYSTEMATIC NAME
IUBMB Comments
adenosine-5'-diphospho-5'-[DNA] hydrolase (adenosine 5'-phosphate-forming)
Aprataxin is a DNA-binding protein involved in different types of DNA break repair. The enzyme acts (among other activities) on abortive DNA ligation intermediates that contain an adenylate covalently linked to the 5'-phosphate DNA terminus. It also acts when the adenylate is covalently linked to the 5'-phosphate of a ribonucleotide linked to a DNA strand, which is the result of abortive ligase activty on products of EC 3.1.26.4, ribonuclease H, an enzyme that cleaves RNA-DNA hybrids on the 5' side of the ribonucleotide found in the 5'-RNA-DNA-3' junction. Aprataxin binds the adenylate group to a histidine residue within the active site, followed by its hydrolysis from the nucleic acid and eventual release, leaving a 5'-phosphate terminus that can be efficiently rejoined. The enzyme also possesses the activities of EC 3.6.1.70, guanosine-5'-diphospho-5'-[DNA] diphosphatase, and EC 3.6.1.72, DNA-3'-diphospho-5'-guanosine diphosphatase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosine-5'-diphospho-5'-[DNA] + H2O
AMP + phospho-5'-[DNA]
show the reaction diagram
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?
additional information
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
primary neural cell
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
APTX_MOUSE
342
0
38723
Swiss-Prot
Mitochondrion (Reliability: 4)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ahel, I.; Rass, U.; El-Khamisy, S.F.; Katyal, S.; Clements, P.M.; McKinnon, P.J.; Caldecott, K.W.; West, S.C.
The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates
Nature
443
713-716
2006
Gallus gallus (P61798), Gallus gallus, Mus musculus (Q7TQC5), Mus musculus
Manually annotated by BRENDA team