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Information on EC 3.6.1.13 - ADP-ribose diphosphatase and Organism(s) Arabidopsis thaliana and UniProt Accession Q94B74
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3.6.1.13 ADP-ribose diphosphataseSpecify your search results
Word Map
- 3.6.1.13
- pyrophosphatases
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melastatin
-
mutt-related
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ribose-5'-phosphate
- adp-sugars
- 8-oxo-dgdp
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
nudt5, nudt9, adp-ribose pyrophosphatase, adprase, atnudt7, adpribase-mn, atnudx7, adp-ribose hydrolase, nudt5 protein, adpr pyrophosphatase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
adenosine diphosphoribose pyrophosphatase
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ADP-ribose diphosphatase
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ADP-ribose phosphohydrolase
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ADP-ribose pyrophosphatase
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ADPR-PPase
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ADPRibase
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ADPribose pyrophosphatase
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pyrophosphatase, adenosine diphosphoribose
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphorous acid anhydride hydrolysis
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SYSTEMATIC NAME
IUBMB Comments
ADP-D-ribose ribophosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9024-83-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT10 shows both ADP-ribose and NADH pyrophosphatase activity
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-
?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT2 shows both ADP-ribose and NADH pyrophosphatase activity
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-
?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT6 shows both ADP-ribose and NADH pyrophosphatase activity
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-
?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT7 shows both ADP-ribose and NADH pyrophosphatase activity
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
improved seed yield parameters and early flowering time upon overexpression of AtNUDX7 in Arabidopsis thaliana. Different levels of constitutive overexpresxadsion of the Arabidopsis AtNUDX7 gene in Arabidopsis and also in maize do not result in a mild drought stress tolerance phenotype. But downregulation of AtNUDX7 results in mild drought stress tolerance under water deficit but growth under normal conditions is reduced, phenotypes, overview
metabolism
the poly(ADP-ribosyl)ation (PAR) pathway is a postxadtranslational protein modification process, activated upon single- or double-stranded DNA breaks, in which ADP-ribose subunits from nicotinamide adenine dinucleotide (NAD+) are covalently attached to target proteins mediated by the poly(ADP-ribose) polymerase enzyme (PARP, EC 2.4.2.30). PARP activity can be reversed by a poly(ADP-ribose) glycohydrolase enzyme (PARG, EC 3.2.1.143) generating free ADP-ribose molecules that can be degraded into adenosine monophosphate (AMP) and ribose-5-phosphate by the ADP-ribose-specific Nudix hydrolase enzyme. AMP can be utilized to replenish the ATP and NAD+, leading to maintenance of cellular homeostasis. Cytosolic enzymes AtNUDX2, AtNUDX6, AtNUDX7, and AtNUDX10 have pyrophosphohydrolase activity toward both ADP-ribose and NADH. Role of the poly(ADP-ribosyl)ation pathway in stress response and energy homeostasis, overview
physiological function
NUDX7 contributes to 23% of the total diphosphohydrolase activity toward ADP-ribose under normal conditions, while under oxidative stress, the contribution of NUDX7 to the activity increases to 34%. Additionally, NUDX7 accounts for 53% of the total pyrophosphohydrolase activity toward NADH under normal conditions and the activity is increased by oxidative stress with NUDX7 contributing 57%
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NUDT2_ARATH
278
0
31611
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
overexpression of AtNUDX7 in Arabidopsis thaliana improves seed parameters, accelerates flowering, and reduces inflorescence height. AtNUDX7 overexpression in Arabixaddopsis might enhance the ADP-ribose recycling step and maintain energy levels by supplying an ATP source in the poly(ADP-ribosyl)ation energy homeostasis pathway. Arabidopsis and maize lines with high, medium and low overexpression levels of the AtNUDX7 gene are analysed in automated platforms and the inhibition of several growth parameters is determined under mild drought stress conditions. The constitutive overexpression of the Arabidopxadsis AtNUDX7 gene in Arabidopsis and maize at varying levels does not improve tolerance to mild drought stress, but knocking down AtNUDX7 expression does, however at the expense of general growth under normal conditions. A loss-of-function mutant line, designated Atnudx7-1 (SALK-046441), has a T-DNA insertion in exon 1 of the AtNUDX7 gene, in the Col-0 background and verified for its T-DNA insertion position, homozygous T-DNA insertion, AtNUDX7 gene expression, and is used as a negative control. Evaluation of the AtNUDX7 overexpression maize lines under mild drought stress
additional information
-
overexpression of AtNUDX7 in Arabidopsis thaliana improves seed parameters, accelerates flowering, and reduces inflorescence height. AtNUDX7 overexpression in Arabixaddopsis might enhance the ADP-ribose recycling step and maintain energy levels by supplying an ATP source in the poly(ADP-ribosyl)ation energy homeostasis pathway. Arabidopsis and maize lines with high, medium and low overexpression levels of the AtNUDX7 gene are analysed in automated platforms and the inhibition of several growth parameters is determined under mild drought stress conditions. The constitutive overexpression of the Arabidopxadsis AtNUDX7 gene in Arabidopsis and maize at varying levels does not improve tolerance to mild drought stress, but knocking down AtNUDX7 expression does, however at the expense of general growth under normal conditions. A loss-of-function mutant line, designated Atnudx7-1 (SALK-046441), has a T-DNA insertion in exon 1 of the AtNUDX7 gene, in the Col-0 background and verified for its T-DNA insertion position, homozygous T-DNA insertion, AtNUDX7 gene expression, and is used as a negative control. Evaluation of the AtNUDX7 overexpression maize lines under mild drought stress
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene AtNUDX7, overexpression of ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7, in several Arabidopsis thaliana and Zea mays transgenic lines under control of the constitutive caulixadflower Mosaic virus 35S promoter and the strong constitutive Brachypodium distachyon pBdEF1a promoter, respectively, quantitative PCR expression analysis. Lines containing the pZmUBIL::AtNUDX7 overexpression construct have generally much lower expression levels than lines driven by the pBdEF1a promoter
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
AtNUDX7 is induced by multiple stresses and is involved in both biotic and abiotic stress responses
oxidative stress caused by 0.003 mM paraquat for 7 days causes an increase in the total ADP-ribose diphosphohydrolase activity of NUDX7
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ogawa, T.; Ueda, Y.; Yoshimura, K.; Shigeoka, S.
Comprehensive analysis of cytosolic Nudix hydrolases in Arabidopsis thaliana
J. Biol. Chem.
280
25277-25283
2005
Arabidopsis thaliana
Ogawa, T.; Ishikawa, K.; Harada, K.; Fukusaki, E.; Yoshimura, K.; Shigeoka, S.
Overexpression of an ADP-ribose pyrophosphatase, AtNUDX2, confers enhanced tolerance to oxidative stress in Arabidopsis plants
Plant J.
57
289-301
2009
Arabidopsis thaliana (Q94B74)
Ishikawa, K.; Yoshimura, K.; Ogawa, T.; Shigeoka, S.
Distinct regulation of Arabidopsis ADP-ribose/NADH pyrophosphohydrolases, AtNUDX6 and 7, in biotic and abiotic stress responses
Plant Signal. Behav.
5
839-841
2010
Arabidopsis thaliana (Q9SU14)
Njuguna, E.; Coussens, E.G.; Neyt, P.; Aesaert, S.; Storme, V.; Demuynck, K.; Vanhaeren, H.; Dhondt, S.; van Haver, Y.; Paul, L.; Inze, D.; Nelissen, H.; van Lijsebettens, M.
Functional analysis of Arabidopsis and maize transgenic lines overexpressing the ADP-ribose/NADH pyrophosphohydrolase, AtNUDX7
Int. J. Dev. Biol.
63
45-55
2019
Arabidopsis thaliana (Q9SU14), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (Q9SU14)
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