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Information on EC 3.5.3.21 - methylenediurea deaminase

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IUBMB Comments
Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea, which then spontaneously hydrolyses to hydroxymethylurea. The enzyme from Ochrobactrum anthropi also hydrolyses dimethylenetriurea and trimethylenetetraurea as well as ureidoglycolate, which is hydrolysed to urea and glyoxylate, and allantoate, which is hydrolysed to ureidoglycolate, ammonia and carbon dioxide.
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The expected taxonomic range for this enzyme is: Ochrobactrum anthropi
Synonyms
methylenediurease, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
methylenediurea + 2 H2O = N-(hydroxymethyl)urea + 2 NH3 + CO2
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The product N-(carboxyaminomethyl)urea decomposes spontaneously into CO2, NH3, formaldehyde and urea according to the reactions: (1) N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2 (2) N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3 (3) N-(hydroxymethyl)urea = urea + formaldehyde
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methylenediurea + H2O = N-(carboxyaminomethyl)urea + NH3
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(1a)
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N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3
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spontaneous
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N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2
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spontaneous
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