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IUBMB Comments Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea, which then spontaneously hydrolyses to hydroxymethylurea. The enzyme from Ochrobactrum anthropi also hydrolyses dimethylenetriurea and trimethylenetetraurea as well as ureidoglycolate, which is hydrolysed to urea and glyoxylate, and allantoate, which is hydrolysed to ureidoglycolate, ammonia and carbon dioxide.
The enzyme appears in viruses and cellular organisms
Synonyms
Deiminase, methylenediurea, MDUase, Methylenediurea deiminase, Methylenediurease,
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Deiminase, methylenediurea
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Methylenediurea deiminase
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MDUase
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Methylenediurease
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methylenediurea + 2 H2O = N-(hydroxymethyl)urea + 2 NH3 + CO2
The product N-(carboxyaminomethyl)urea decomposes spontaneously into CO2, NH3, formaldehyde and urea according to the reactions: (1) N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2 (2) N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3 (3) N-(hydroxymethyl)urea = urea + formaldehyde
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methylenediurea + H2O = N-(carboxyaminomethyl)urea + NH3
(1a)
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N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3
spontaneous
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N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2
spontaneous
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hydrolysis of linear amidines
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methylenediurea aminohydrolase
Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea, which then spontaneously hydrolyses to hydroxymethylurea. The enzyme from Ochrobactrum anthropi also hydrolyses dimethylenetriurea and trimethylenetetraurea as well as ureidoglycolate, which is hydrolysed to urea and glyoxylate, and allantoate, which is hydrolysed to ureidoglycolate, ammonia and carbon dioxide.
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Allantoate + H2O
Glyoxylate + urea + ammonium
dimethylenetriurea + H2O
ammonium + formaldehyde + urea + CO2
dimethylenetriurea + H2O
urea + NH3 + formaldehyde + CO2
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methylenediurea + H2O
ammonium + formaldehyde + urea + CO2
methylenediurea + H2O
urea + NH3 + formaldehyde + CO2
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trimethylenetetraurea + H2O
ammonium + formaldehyde + urea + CO2
Ureidoglycolate + H2O
Glyoxylate + urea
Allantoate + H2O
Glyoxylate + urea + ammonium
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at 5% of the activity with methylenediurea
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Allantoate + H2O
Glyoxylate + urea + ammonium
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at 5% of the activity with methylenediurea
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dimethylenetriurea + H2O
ammonium + formaldehyde + urea + CO2
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ammonium:formaldehyde:urea at a molar ratio of 4:2:1
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dimethylenetriurea + H2O
ammonium + formaldehyde + urea + CO2
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ammonium:formaldehyde:urea at a molar ratio of 4:2:1
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methylenediurea + H2O
ammonium + formaldehyde + urea + CO2
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ammonium:formaldehyde:urea at a molar ratio of 2:1:1
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methylenediurea + H2O
ammonium + formaldehyde + urea + CO2
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ammonium:formaldehyde:urea at a molar ratio of 2:1:1
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trimethylenetetraurea + H2O
ammonium + formaldehyde + urea + CO2
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ammonium:formaldehyde:urea at a molar ratio of 6:3:1
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trimethylenetetraurea + H2O
ammonium + formaldehyde + urea + CO2
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ammonium:formaldehyde:urea at a molar ratio of 6:3:1
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Ureidoglycolate + H2O
Glyoxylate + urea
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at 130% of the activity with methylenediurea
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Ureidoglycolate + H2O
Glyoxylate + urea
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at 130% of the activity with methylenediurea
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0.0004
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with NH4Cl as nitrogen source
0.97
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with methylenurea as nitrogen source
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7 - 9.5
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pH 7: about 25% of maximal activity, pH 9.5: about 70% of maximal activity
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30 - 70
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30°C: about 45% of maximal activity, 70°C: about 30% of maximal activity
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brenda
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brenda
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brenda
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38000
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4 * 38000, SDS-PAGE
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tetramer
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4 * 38000, SDS-PAGE
tetramer
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4 * 38000, SDS-PAGE
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5 - 11
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60 min, stable
32167
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70
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30 min, stable up to
80
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30 min, complete loss of activity
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Jahns, T.; Schepp, R.; Kaltwasser, H.
Purification and characterization of an enzyme from a strain of Ochrobactrum anthropi that degrades condensation products of urea and formaldehyde (ureaform)
Can. J. Microbiol.
43
1111-1117
1997
Ochrobactrum anthropi, Ochrobactrum anthropi UF4
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brenda
Jahns, T.; Schepp, R.; Siersdorfer, C.; Kaltwasser, H.
Microbial urea-formaldehyde degradation involves a new enzyme, methylenediurease
Acta Biol. Hung.
49
449-454
1998
Ochrobactrum anthropi
brenda
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