Information on EC 3.5.3.21 - methylenediurea deaminase

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The expected taxonomic range for this enzyme is: Ochrobactrum anthropi

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EC NUMBER
COMMENTARY hide
3.5.3.21
-
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RECOMMENDED NAME
GeneOntology No.
methylenediurea deaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
methylenediurea + 2 H2O = N-(hydroxymethyl)urea + 2 NH3 + CO2
show the reaction diagram
The product N-(carboxyaminomethyl)urea decomposes spontaneously into CO2, NH3, formaldehyde and urea according to the reactions: (1) N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2 (2) N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3 (3) N-(hydroxymethyl)urea = urea + formaldehyde
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-
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methylenediurea + H2O = N-(carboxyaminomethyl)urea + NH3
show the reaction diagram
(1a)
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-
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N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3
show the reaction diagram
spontaneous
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-
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N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2
show the reaction diagram
spontaneous
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amidines
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
methylenediurea aminohydrolase
Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea, which then spontaneously hydrolyses to hydroxymethylurea. The enzyme from Ochrobactrum anthropi also hydrolyses dimethylenetriurea and trimethylenetetraurea as well as ureidoglycolate, which is hydrolysed to urea and glyoxylate, and allantoate, which is hydrolysed to ureidoglycolate, ammonia and carbon dioxide.
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CAS REGISTRY NUMBER
COMMENTARY hide
205830-62-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Allantoate + H2O
Glyoxylate + urea + ammonium
show the reaction diagram
dimethylenetriurea + H2O
ammonium + formaldehyde + urea + CO2
show the reaction diagram
dimethylenetriurea + H2O
urea + NH3 + formaldehyde + CO2
show the reaction diagram
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-
-
-
?
methylenediurea + H2O
ammonium + formaldehyde + urea + CO2
show the reaction diagram
methylenediurea + H2O
urea + NH3 + formaldehyde + CO2
show the reaction diagram
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-
-
-
?
trimethylenetetraurea + H2O
ammonium + formaldehyde + urea + CO2
show the reaction diagram
Ureidoglycolate + H2O
Glyoxylate + urea
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Acetohydroxamate
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0004
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with NH4Cl as nitrogen source
0.97
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with methylenurea as nitrogen source
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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pH 7: about 25% of maximal activity, pH 9.5: about 70% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 70
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30°C: about 45% of maximal activity, 70°C: about 30% of maximal activity
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
4 * 38000, SDS-PAGE
125000
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native PAGE
140000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11
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60 min, stable
32167
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
30 min, stable up to
80
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30 min, complete loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 3.5.3.21)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)