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Specific for the hydrolysis of the gamma-amide of glutamine substituted at the alpha-amino group, e.g., glycyl-L-glutamine, N-acetyl-L-glutamine and L-leucylglycyl-L-glutamine.
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The expected taxonomic range for this enzyme is: Bacillus circulans
peptidoglutaminase, peptidoglutaminase i, more
alpha-N-peptidyl-L-glutamine + H2O = alpha-N-peptidyl-L-glutamate + NH3
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