Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.24.36 - leishmanolysin and Organism(s) Leishmania chagasi and UniProt Accession P15706

for references in articles please use BRENDA:EC3.4.24.36
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.36 leishmanolysin
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Leishmania chagasi
UNIPROT: P15706 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Leishmania chagasi
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-/-Leu-Lys-Lys-
Synonyms
major surface glycoprotein, cell surface protease, leishmanolysin, major surface protease, surface protease gp63, glycoprotein gp63, promastigote surface protease, metalloprotease gp63, glycoprotein 63, gp63 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
virulence factor major surface protease
-
Cell surface protease
-
-
-
-
Glycoprotein gp63
-
-
-
-
GP63 protein
-
-
-
-
Leishmania metalloproteinase
-
-
-
-
Major surface glycoprotein
-
-
-
-
major surface protease
-
-
Promastigote surface endopeptidase
-
-
-
-
Surface acid proteinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
161052-06-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
major surface protease is a virulence factor of Leishmania spp. protozoan, virulent Leishmania chagasi undergoes a growth-associated lengthening in the t1/2 of surface-localized MSP, but this does not occur in the attenuated L5 strain, surface-localized MSP isoforms are differently regulated in attenuated and virulent strains, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
major surface protease is a virulence factor of Leishmania spp. protozoan, virulent Leishmania chagasi undergoes a growth-associated lengthening in the t1/2 of surface-localized MSP, but this does not occur in the attenuated L5 strain, surface-localized MSP isoforms are differently regulated in attenuated and virulent strains, overview
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
MSPs are metalloproteinases
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
amastigote MSPS
6 - 8
-
promastigote MSPS
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.65
-
MSPS and MSPL
5.8 - 6.7
-
8 different bands of isozymes within this range
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
MSP multiple forms expression analysis during parasite life stages, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
metacyclic promastigote exosomes contain the highest, and logarithmic exosomes have the lowest abundance of total major surface protease GP63. Among the major surface protease classes, major surface protease C class has the greatest variety of isoforms, but is least abundant in all exosomes. All major surface protease classes are present at higher levels in exosomes released from stationary or metacyclic promastigotes than logarithmic promastigotes
-
Manually annotated by BRENDA team
-
most MSPs in promastigotes are membrane-associated, whereas most MSPs in amastigotes are cytosolic
Manually annotated by BRENDA team
-
two-third of MSPs in promastigotes is orientated along the cell surface, whereas most MSPs in amastigotes are localized in the flagellar pocket
-
Manually annotated by BRENDA team
additional information
-
three pools of MSP, located either internally within the parasite, anchored to the surface membrane, or released into the extracellular environment, promastigotes incubation with Matrigel matrix, a soluble basement membrane extract of Engelbreth-Holm-Swarm tumor cells, stimulates the release of internal MSP but not of surface-located MSP, at 37°C, overview
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GP63_LEICH
599
1
63848
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51674
-
x * 59177, MSPC, sequence calculation, x * 51689, MSPS, sequence calculation, x * 51674, MSPL, sequence calculation
51689
-
x * 59177, MSPC, sequence calculation, x * 51689, MSPS, sequence calculation, x * 51674, MSPL, sequence calculation
59177
-
x * 59177, MSPC, sequence calculation, x * 51689, MSPS, sequence calculation, x * 51674, MSPL, sequence calculation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 59177, MSPC, sequence calculation, x * 51689, MSPS, sequence calculation, x * 51674, MSPL, sequence calculation
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
posttranslational modifications of enzyme isoforms include N-glycosylation, GPI anchor addition and phosphorylation
phosphoprotein
posttranslational modifications of enzyme isoforms include N-glycosylation, GPI anchor addition and phosphorylation
glycoprotein
-
most MSPs in promastigotes are N-glycosylated, while in amastigotes they are not
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yao, C.; Luo, J.; Storlie, P.; Donelson, J.E.; Wilson, M.E.
Multiple products of the Leishmania chagasi major surface protease (MSP or GP63) gene family
Mol. Biochem. Parasitol.
135
171-183
2004
Leishmania chagasi (P15706), Leishmania chagasi
Manually annotated by BRENDA team
Yao, C.; Luo, J.; Hsiao, C.; Donelson, J.E.; Wilson, M.E.
Internal and surface subpopulations of the major surface protease (MSP) of Leishmania chagasi
Mol. Biochem. Parasitol.
139
173-183
2005
Leishmania chagasi
Manually annotated by BRENDA team
Yao, C.; Donelson, J.E.; Wilson, M.E.
Internal and surface-localized major surface proteases of Leishmania spp. and their differential release from promastigotes
Eukaryot. Cell
6
1905-1912
2007
Leishmania chagasi, Leishmania chagasi MHOM/BR/00/1669
Manually annotated by BRENDA team
Hsiao, C.C.; Yao, C.; Storlie, P.; Donelson, J.E.; Wilson, M.E.
The major surface protease (MSP or GP63) in the intracellular amastigote stage of Leishmania chagasi
Mol. Biochem. Parasitol.
157
148-159
2008
Leishmania chagasi, Leishmania chagasi MHOM/BR/00/1669
Manually annotated by BRENDA team
Marshall, S.; Kelly, P.H.; Singh, B.K.; Pope, R.M.; Kim, P.; Zhanbolat, B.; Wilson, M.E.; Yao, C.
Extracellular release of virulence factor major surface protease via exosomes in Leishmania infantum promastigotes
Parasit. Vectors
11
355
2018
Leishmania amazonensis (Q27673), Leishmania chagasi (P15706), Leishmania donovani (P23223), Leishmania donovani (Q25274), Leishmania donovani (Q967C0), Leishmania donovani (Q967C2), Leishmania donovani donovani (F0V646), Leishmania donovani donovani (F0V651), Leishmania infantum (A4HUF6), Leishmania infantum (A4HUF9), Leishmania infantum (A4HUG0), Leishmania infantum (E9AHH5), Leishmania infantum, Leishmania major (P08148), Leishmania mexicana (E9AN53), Leishmania mexicana (P43150), Leishmania mexicana U1103 (E9AN53), Leishmania tropica (Q8MNZ1)
Manually annotated by BRENDA team