Any feedback?
Information on EC 3.4.22.B26 - calpain 6
for references in articles please use BRENDA:EC3.4.22.B26preliminary BRENDA-supplied EC number
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.4.22.B26 calpain 6Specify your search results
Word Map
- 3.4.22.B26
-
non-proteolytic
- medicine
-
myometrium
- leiomyomas
-
nonlysosomal
-
pinocytotic
- calpastatin
The enzyme appears in viruses and cellular organisms
Reaction Schemes
A microtubule-stabilizing protein. The enzyme lacks the active-site catalytic cysteine residue and proteolytic activity has not been detected.
Synonyms
capn6, calpain-6, calpain 6, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
calpain-6 expression is associated with cancer stem cell features. In mouse models of bone sarcoma, calpain-6-expressing cells have unique tumor-initiating and metastatic capacities. Calpain-6 levels are especially high in tumors that have been successfully propagated in mouse to establish patient-derived xenografts. Calpain-6 knockdown blocks tumor development in mouse and induces depletion of the cancer stem cell population. Calpain-6 levels are increased by hypoxia in vitro and calpain-6 is detected within hypoxic areas in tumors. Calpain-6 expression depends on the stem cell transcription network that involves Oct4, Nanog, and Sox2 and is activated by hypoxia. Calpain-6 expression in sarcomas inversely correlates with senescence markers. Calpain-6 knockdown suppresses hypoxia-dependent prevention of senescence entry and also promotion of autophagic flux
physiological function
inflammatory cytokines induce nonproteolytic calpain-6 (CAPN6), which associates with the essential exon junction complex-loading factor CWC22 in the cytoplasm. This association disturbs the nuclear localization of CWC22, thereby suppressing the splicing of target genes, including those related to Rac1 signaling. CAPN6 deficiency in low density lipoprotein receptor-deficient mice restores CWC22/EJC/Rac1 signaling, reduces pinocytotic deposition of native low density lipoprotein in macrophages, and attenuates macrophage recruitment into the lesions, generating an atheroprotective phenotype in the aorta. In macrophages, the induction of CAPN6 in the atheroma interior limits macrophage movements, resulting in a decline in cell clearance from the lesions. Myeloid CAPN6 contributes to atherogenesis in a murine model of bone marrow transplantation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CAN6_MOUSE
641
0
74543
Swiss-Prot
Mitochondrion (Reliability: 3)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
calpain-6 expression is associated with cancer stem cell features. In mouse models of bone sarcoma, calpain-6-expressing cells have unique tumor-initiating and metastatic capacities. Calpain-6 levels are especially high in tumors that have been successfully propagated in mouse to establish patient-derived xenografts. Calpain-6 levels are increased by hypoxia in vitro and calpain-6 is detected within hypoxic areas in tumors. Calpain-6 knockdown blocks tumor development in mouse and induces depletion of the cancer stem cell population. Calpain-6 expression depends on the stem cell transcription network that involves Oct4, Nanog, and Sox2 and is activated by hypoxia. Calpain-6 expression in sarcomas inversely correlates with senescence markers. Calpain-6 knockdown suppresses hypoxia-dependent prevention of senescence entry and also promotion of autophagic flux
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tonami, K.; Kurihara, Y.; Aburatani, H.; Uchijima, Y.; Asano, T.; Kurihara, H.
Calpain 6 is involved in microtubule stabilization and cytoskeletal organization
Mol. Cell. Biol.
27
2548-2561
2007
Mus musculus (O35646)
Miyazaki, T.; Tonami, K.; Hata, S.; Aiuchi, T.; Ohnishi, K.; Lei, X.F.; Kim-Kaneyama, J.R.; Takeya, M.; Itabe, H.; Sorimachi, H.; Kurihara, H.; Miyazaki, A.
Calpain-6 confers atherogenicity to macrophages by dysregulating pre-mRNA splicing
J. Clin. Invest.
126
3417-3432
2016
Mus musculus (O35646), Mus musculus
Andrique, C.; Morardet, L.; Linares, L.K.; Cisse, M.Y.; Merle, C.; Chibon, F.; Provot, S.; Hay, E.; Ea, H.K.; Cohen-Solal, M.; Modrowski, D.
Calpain-6 controls the fate of sarcoma stem cells by promoting autophagy and preventing senescence
JCI Insight
3
121225
2018
Homo sapiens (Q9Y6Q1), Mus musculus (O35646), Mus musculus
EXTERNAL LINKS (specific for EC-Number 3.4.22.B26)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
