Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.22.B25 - calpain 5 and Organism(s) Rattus norvegicus and UniProt Accession Q8R4C0

for references in articles please use BRENDA:EC3.4.22.B25
preliminary BRENDA-supplied EC number
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B25 calpain 5
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
UNIPROT: Q8R4C0
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
broad endopeptidase specificity
Synonyms
capn5, calpain-5, calpain 5, htra-3, ncl-3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calpain htra-3
-
-
-
-
htra-3
-
-
-
-
nCL-3
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
dependent on
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression level
Manually annotated by BRENDA team
weak expression
Manually annotated by BRENDA team
strong expression
Manually annotated by BRENDA team
strong expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the enzyme carries dual nuclear localization signals
Manually annotated by BRENDA team
additional information
unlike findings in classical calpains, which are characterized as being mainly cytosolic, CAPN5 is predominantly a noncytosolic calpain, present in the nucleus in the nucleic acidbinding protein fraction. The enzyme is associated with nuclear promyelocytic leukemia protein bodies
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
calpain 5 is a non-classical member of the calpain family. It lacks the EF hand motif characteristic of classical calpains but retains catalytic and Ca2 binding domains, and it contains a unique C-terminal domain
additional information
the enzyme is associated with nuclear promyelocytic leukemia protein bodies
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAN5_RAT
640
0
73065
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
76000
calculated from amino acid sequence
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Capn5, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Mus musculus (O08688), Rattus norvegicus (Q8R4C0), Rattus norvegicus Sprague-Dawley (Q8R4C0)
Manually annotated by BRENDA team
Waghray, A.; Wang, D.S.; McKinsey, D.; Hayes, R.L.; Wang, K.K.
Molecular cloning and characterization of rat and human calpain-5
Biochem. Biophys. Res. Commun.
324
46-51
2004
Homo sapiens (O15484), Homo sapiens, Rattus norvegicus (Q8R4C0)
Manually annotated by BRENDA team
Singh, R.; Brewer, M.K.; Mashburn, C.B.; Lou, D.; Bondada, V.; Graham, B.; Geddes, J.W.
Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies
J. Biol. Chem.
289
19383-19394
2014
Homo sapiens (O15484), Mus musculus (O08688), Mus musculus C57/BL6J (O08688), Rattus norvegicus (Q8R4C0), Rattus norvegicus Sprague-Dawley (Q8R4C0)
Manually annotated by BRENDA team