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Information on EC 3.4.22.40 - bleomycin hydrolase and Organism(s) Mus musculus and UniProt Accession Q8R016

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.40 bleomycin hydrolase
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This record set is specific for:
Mus musculus
UNIPROT: Q8R016 not found.
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
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Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
Synonyms
blh, bleomycin hydrolase, blm hydrolase, htlase, homocysteine thiolactonase, hcy-thiolactonase, aminopeptidase c, aminopeptidase h, hydrolase h, bana-hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
BH protein
-
BLMH protein
-
aminopeptidase C
-
-
-
-
aminopeptidase H
-
-
-
-
BANA-hydrolase
-
-
-
-
Bleomycin hydrolase
-
-
-
-
Blh1p
-
-
-
-
BLM hydrolase
-
-
-
-
BMH
-
-
-
-
Gal6p
-
-
-
-
hydrolase H
-
-
-
-
PEPC
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
53096-17-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Lys(2-Cl-benzyloxycarbonyl)-ACC + H2O
?
show the reaction diagram
-
-
-
?
bleomycin + H2O
deamido-bleomycin
show the reaction diagram
homocysteine-thiolactone + H2O
?
show the reaction diagram
-
-
-
-
?
L-homocysteine-thiolactone + H2O
L-homocysteine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
homocysteine-thiolactone + H2O
?
show the reaction diagram
-
-
-
-
?
L-homocysteine-thiolactone + H2O
L-homocysteine
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2-chlorophenyl)methyl [(5S)-5-amino-6-oxo-7-(2,3,5,6-tetrafluorophenoxy)heptyl]carbamate
-
(2-chlorophenyl)methyl [(5S,6E)-5-amino-7-(benzenesulfonyl)hept-6-en-1-yl]carbamate
-
(2-chlorophenyl)methyl [(5S,6E)-5-amino-7-(methanesulfonyl)hept-6-en-1-yl]carbamate
-
(2R,3E)-1-(benzylsulfanyl)-4-(methanesulfonyl)but-3-en-2-amine
-
(2R,3E)-4-(benzenesulfonyl)-1-(benzylsulfanyl)but-3-en-2-amine
-
(3S)-3-amino-7-([[(2-chlorophenyl)methoxy]carbonyl]amino)-2-oxoheptyl 2,6-dimethylbenzoate
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000077
(2-chlorophenyl)methyl [(5S)-5-amino-6-oxo-7-(2,3,5,6-tetrafluorophenoxy)heptyl]carbamate
Mus musculus
37°C, pH 7.12
0.00056
(2-chlorophenyl)methyl [(5S,6E)-5-amino-7-(benzenesulfonyl)hept-6-en-1-yl]carbamate
Mus musculus
37°C, pH 7.8
0.000378
(2-chlorophenyl)methyl [(5S,6E)-5-amino-7-(methanesulfonyl)hept-6-en-1-yl]carbamate
Mus musculus
37°C, pH 7.7
0.0039
(2R,3E)-1-(benzylsulfanyl)-4-(methanesulfonyl)but-3-en-2-amine
Mus musculus
37°C, pH 7.5
0.0025
(2R,3E)-4-(benzenesulfonyl)-1-(benzylsulfanyl)but-3-en-2-amine
Mus musculus
37°C, pH 7.6
0.0000054
(3S)-3-amino-7-([[(2-chlorophenyl)methoxy]carbonyl]amino)-2-oxoheptyl 2,6-dimethylbenzoate
Mus musculus
37°C, pH 7.11
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
histological examination, increase in GFAP-positively stained astroglia observed in the brains of aged null mice compared with wild-type controls
Manually annotated by BRENDA team
MEFs, bleomycin hydrolase-deficient cells from mice
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
-
bleomycin hydrolase protects against L-homocysteine-thiolactone toxicity in brain and kidney
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BLMH_MOUSE
455
0
52511
Swiss-Prot
other Location (Reliability: 2)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schwartz, D.R.; Homanics, G.E.; Hoyt, D.G.; Klein, D.G.; Klein, E.; Abernethy, J.; Lazo, J.S.
The neutral cysteine protease bleomycin hydrolase is essential for epidermal integrity and bleomycin resistance
Proc. Natl. Acad. Sci. USA
96
4680-4685
1999
Mus musculus
Manually annotated by BRENDA team
Sebti, S.M.; Mignano, J.E.; Jani, J.P.; Srimatkandada, S.; Lazo, J.S.
Bleomycin hydrolase: molecular cloning, sequencing, and biochemical studies reveal membership in the cysteine proteinase family
Biochemistry
28
6544-6548
1989
Gallus gallus, Homo sapiens, Lithobates catesbeianus, Mus musculus, Oryctolagus cuniculus, Papio hamadryas, Rattus norvegicus
Manually annotated by BRENDA team
Towne, C.F.; York, I.A.; Watkin, L.B.; Lazo, J.S.; Rock, K.L.
Analysis of the role of bleomycin hydrolase in antigen presentation and the generation of CD8 T cell responses
J. Immunol.
178
6923-6930
2007
Mus musculus (Q8R016)
Manually annotated by BRENDA team
Montoya, S.E.; Thiels, E.; Card, J.P.; Lazo, J.S.
Astrogliosis and behavioral changes in mice lacking the neutral cysteine protease bleomycin hydrolase
Neuroscience
146
890-900
2007
Mus musculus (Q8R016)
Manually annotated by BRENDA team
Borowczyk, K.; Tisonczyk, J.; Jakubowski, H.
Metabolism and neurotoxicity of homocysteine thiolactone in mice: protective role of bleomycin hydrolase
Amino Acids
43
1339-1348
2012
Mus musculus
Manually annotated by BRENDA team
Suszynska-Zajczyk, J.; Wroblewski, J.; Utyro, O.; Luczak, M.; Marczak, L.; Jakubowski, H.
Bleomycin hydrolase and hyperhomocysteinemia modulate the expression of mouse proteins involved in liver homeostasis
Amino Acids
46
1471-1480
2014
Mus musculus
Manually annotated by BRENDA team
van der Linden, W.A.; Segal, E.; Child, M.A.; Byzia, A.; Drag, M.; Bogyo, M.
Design and synthesis of activity-based probes and inhibitors for bleomycin hydrolase
Chem. Biol.
22
995-1001
2015
Mus musculus (Q8R016)
Manually annotated by BRENDA team