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Information on EC 3.4.21.B5 - mast cell protease 5 and Organism(s) Mus musculus and UniProt Accession P21844

for references in articles please use BRENDA:EC3.4.21.B5
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B5 mast cell protease 5
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This record set is specific for:
Mus musculus
UNIPROT: P21844
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
endoprotease activity
Synonyms
mmcp-5, rmcp-5, mast cell protease-5, mast cell protease 5, rat mast cell protease 5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mast cell protease-5
-
S01.150
Merops ID
mast cell chymases 5
-
-
MCP-5
-
MCP-5 is a alpha-chymase
MMCP-5'
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
cleavage of C-N-linkage
-
hydrolysis of peptide bond
cleavage of C-N-linkage
CAS REGISTRY NUMBER
COMMENTARY hide
142539-77-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Fibronectin + H2O
?
show the reaction diagram
-
-
-
?
Protein + H2O
?
show the reaction diagram
-
-
?
Protein + H2O
?
show the reaction diagram
additional information
?
-
-
the enzyme mediates ischemia-reperfusion injury of mouse skeletal muscle
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Fibronectin + H2O
?
show the reaction diagram
-
-
-
?
Protein + H2O
?
show the reaction diagram
-
-
?
Protein + H2O
?
show the reaction diagram
additional information
?
-
-
the enzyme mediates ischemia-reperfusion injury of mouse skeletal muscle
-
-
?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.4
-
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
experimental arthritis is markedly reduced in enzyme-deficient mice relative to wild type mice
metabolism
the enzyme is needed to target translated mast cell carboxypeptidase A to the secretory granule along with heparin-containing serglycin proteoglycans. Alternately, the enzyme is needed to protect mast cell carboxypeptidase A from autolysis in the cell's granules
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CMA1_MOUSE
247
0
27586
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25400
calculated from amino acid sequence
25000 - 26000
-
SDS-PAGE
25270
-
calculated from amino acid sequence
30000
-
SDS-PAGE, MMCP-5'in serosal mast cells
31000
-
SDS-PAGE, MMCP-5 in serosal mast cells and Kirsten sarcoma virus-immortalized mast cells
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
allelic variant MMCP-5.1
allelic variant MMCP-5.4
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chu, W.; Johnson, D.A.; Musich, P.R.
Molecular cloning and characterization of mouse mast cell chymases
Biochim. Biophys. Acta
1121
83-87
1992
Mus musculus (P21844), Mus musculus (Q8VDE7)
Manually annotated by BRENDA team
Huang, R.Y.; Blom, T.; Hellman, L.
Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases
Eur. J. Immunol.
21
1611-1621
1991
Mus musculus
Manually annotated by BRENDA team
McNeil, H.P.; Austen, K.F.; Somerville, L.L.; Gurish, M.F.; Stevens, R.L.
Molecular cloning of the mouse mast cell protease-5 gene. A novel secretory granule protease expressed early in the differentiation of serosal mast cells
J. Biol. Chem.
266
20316-20322
1991
Mus musculus (P21844), Mus musculus
Manually annotated by BRENDA team
Reynolds, D.S.; Stevens, R.L.; Lane, W.S.; Carr, M.H.; Austen, K.F.; Serafin, W.E.
Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases
Proc. Natl. Acad. Sci. USA
87
3230-3234
1990
Mus musculus
Manually annotated by BRENDA team
Abonia, J.P.; Friend, D.S.; Austen, W.G.; Moore, F.D.; Carroll, M.C.; Chan, R.; Afnan, J.; Humbles, A.; Gerard, C.; Knight, P.; Kanaoka, Y.; Yasuda, S.; Morokawa, N.; Austen, K.F.; Stevens, R.L.; Gurish, M.F.
Mast cell protease 5 mediates ischemia-reperfusion injury of mouse skeletal muscle
J. Immunol.
174
7285-7291
2005
Mus musculus
Manually annotated by BRENDA team
Simard, E.; Jin, D.; Takai, S.; Miyazaki, M.; Brochu, I.; DOrleans-Juste, P.
Chymase-dependent conversion of Big endothelin-1 in the mouse in vivo
J. Pharmacol. Exp. Ther.
328
540-548
2009
Mus musculus
Manually annotated by BRENDA team
Stevens, R.L.; McNeil, H.P.; Wensing, L.A.; Shin, K.; Wong, G.W.; Hansbro, P.M.; Krilis, S.A.
Experimental arthritis is dependent on mouse mast cell protease-5
J. Biol. Chem.
292
5392-5404
2017
Mus musculus (P21844), Mus musculus
Manually annotated by BRENDA team