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Information on EC 3.4.19.5 - beta-aspartyl-peptidase and Organism(s) Homo sapiens and UniProt Accession Q7L266

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.5 beta-aspartyl-peptidase
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q7L266 not found.
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide
Synonyms
ntn-hydrolase, isoaspartyl dipeptidase, hasrgl1, cpsiada, asparaginase-like protein 1, isoaspartyl peptidase/l-asparaginase, beta-aspartyl peptidase, isoaspartyl peptidase, isoaspartyl aminopeptidase/asparaginase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
asparaginase-like protein 1
-
beta-aspartyl dipeptidase
-
-
-
-
beta-aspartyl peptidase
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-
-
-
dipeptidase, beta-aspartyl
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
37288-74-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-L-Asp-L-Ala + H2O
L-Asp + L-Ala
show the reaction diagram
-
-
-
?
beta-L-Asp-L-Leu + H2O
L-Asp + L-Leu
show the reaction diagram
-
-
-
?
beta-L-Asp-L-Lys + H2O
L-Asp + L-Lys
show the reaction diagram
-
-
-
?
beta-L-Asp-L-Phe + H2O
L-Asp + L-Phe
show the reaction diagram
-
-
-
?
beta-L-Asp-L-Phe methyl ester + H2O
L-Asp + L-Phe methyl ester
show the reaction diagram
-
-
-
?
L-Asp beta-methyl ester + H2O
L-Asp + methanol
show the reaction diagram
-
-
-
?
L-aspartic acid beta-(7-amido-4-methylcoumarin) + H2O
L-aspartic acid + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ASGL1_HUMAN
308
0
32055
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14550
mass spectroscopy, beta-subunit
18000
Western blot, reducing conditions, detected in soluble and insoluble fractions
18500
mass spectroscopy, alpha-subunit
33000
Western blot, reducing conditions, detected in soluble and insoluble fractions
33020
mass spectroscopy, precursor enzyme
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T168A
mutant does not show enzymatic activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography. hASRGL1 purification by immobilized metal ion affinity chromatography yields 30 mg of protein/l with a purity of more than 90% as determined by SDS-PAGE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cantor, J.R.; Stone, E.M.; Chantranupong, L.; Georgiou, G.
The human asparaginase-like protein 1 hASRGL1 is an Ntn hydrolase with beta-aspartyl peptidase activity
Biochemistry
48
11026-11031
2009
Homo sapiens (Q7L266), Homo sapiens
Manually annotated by BRENDA team