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IUBMB CommentsUnlike EC 3.2.2.9, adenosylhomocysteine nucleosidase, this plant enzyme has little or no activity with S-adenosyl-L-homocysteine.
The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
5'-methylthioadenosine/s-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine nucleosidase, mta/sah nucleosidase, methylthioadenosine nucleosidase, mta nucleosidase, bgp protein, methylthioadenosine/s-adenosylhomocysteine nucleosidase, glycosaminoglycan-binding protein, 5-methylthioadenosine/s-adenosylhomocysteine nucleosidase, s-adenosylhomocysteine/5'-methylthioadenosine nucleosidase,
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5-methylthioadenosine nucleosidase
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5'-methylthioadenosine nucleosidase
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5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
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MeSAdo nucleosidase
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hydrolysis of N-glycosyl bond
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S-methyl-5'-thioadenosine adeninehyrolase
Unlike EC 3.2.2.9, adenosylhomocysteine nucleosidase, this plant enzyme has little or no activity with S-adenosyl-L-homocysteine.
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5'-methylthioadenosine + H2O
adenine + S-methyl-5-thio-D-ribose
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S-methyl-5'-thioadenosine + H2O
S-methyl-5-thio-D-ribose + adenine
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S-methyl-5'-thioadenosine + H2O
S-methyl-5-thio-D-ribose + adenine
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10 - 12
recombinant His-tagged enzyme
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37
recombinant His-tagged enzyme
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gene RV0091
UniProt
brenda
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metabolism
the enzyme plays a key role in the metabolism of Mycobacterium tuberculosis
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7.5 - 12
recombinant His-tagged enzyme, stable at, denaturation and loss of activity at and below pH 7.0
731666
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additional information
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the purified recombinant His-tagged enzyme shows a poor thermal stability
additional information
the purified recombinant His-tagged enzyme shows a poor thermal stability
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recombinant His-tagged enzyme from Escherichia coli strain BL21trxB by nickel affinity chromatography to over 95% purity
Ni-NTA column chromatography, and gel filtration
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gene RV0091, recombinant expression of active His-tagged enzyme in Escherichia coli strain BL21trxB, optimization of expression method
expressed in Escherichia coli BL21trxB cells
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Chen, H.; Yang, H.; Hu, Z.; Yang, H.; Ma, H.; Gao, S.; Guo, Q.; Bai, W.; Qin, L.; Li, L.
Prokaryotic expression and activity analysis of 5'-methylthioadenosine nucleosidase in Mycobacterium tuberculosis
Chin. J. Microbiol. Immunol.
32
589-594
2012
Mycobacterium tuberculosis, Mycobacterium tuberculosis (P9WJN3)
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brenda
3.2.2.16 methylthioadenosine nucleosidase
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