Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.2.1.198 - alpha-mannan endo-1,2-alpha-mannanase

for references in articles please use BRENDA:EC3.2.1.198
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
The enzyme, characterized from the gut bacteria Bacteroides thetaiotaomicron and Bacteroides xylanisolvens, can also catalyse the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
UNIPROT: D6D1V7
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The expected taxonomic range for this enzyme is: Bacteroides
Reaction Schemes
Hydrolysis of the terminal alpha-D-mannosyl-(1->3)-alpha-D-mannose disaccharide from alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl side chains in fungal cell wall alpha-mannans
Synonyms
BT_3862, BXY_34140, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
alpha-mannan 1,2-[alpha-D-mannosyl-(1->3)-alpha-D-mannose] hydrolase
The enzyme, characterized from the gut bacteria Bacteroides thetaiotaomicron and Bacteroides xylanisolvens, can also catalyse the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
show the reaction diagram
-
-
-
?
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
show the reaction diagram
-
-
-
?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
show the reaction diagram
-
-
-
?
additional information
?
-
enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-mannopyranosyl-1,3-isofagomine
Kd value 140 nm
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
pH 7, 37°C
0.404
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
pH 7, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
pH 7, 37°C
0.0008
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
pH 7, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0085
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
pH 7, 37°C
0.0021
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
pH 7, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GH99 family protein
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme releases alpha-1,3-mannobiose by hydrolyzing alphaMan-1,3-aMan->1,2-alphaMan-1,2-alphaMan sequences within branches off the main alpha-1,6-mannan backbone of fungal cell walls
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
D6D1V7_9BACE
380
0
43437
TrEMBL
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with inhibitor alpha-mannopyranosyl-1,3-isofagomine. The inhibitor is coordinated by residues Y46, W48, H63, W126, E156, E333 and E336
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hakki, Z.; Thompson, A.J.; Bellmaine, S.; Speciale, G.; Davies, G.J.; Williams, S.J.
Structural and kinetic dissection of the endo-alpha-1,2-mannanase activity of bacterial GH99 glycoside hydrolases from Bacteroides spp.
Chemistry
21
1966-1977
2015
Bacteroides thetaiotaomicron (Q8A109), Bacteroides thetaiotaomicron DSM 2079 (Q8A109), Bacteroides xylanisolvens (D6D1V7)
Manually annotated by BRENDA team