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protein serine/threonine phosphatase
-
serine/threonine protein phosphatase
-
3-hydroxy 3-methylglutaryl CoenzymeA reductase phosphatase
-
-
-
-
Aspergillus awamori acid protein phosphatase
-
-
-
-
BCKDH phosphatase
-
-
-
-
branched-chain alpha-keto acid dehydrogenase phosphatase
-
-
-
-
CaM-kinase phosphatase
-
-
-
-
casein phosphatase
-
-
-
-
Fibroblast growth factor inducible protein 13
-
-
-
-
Flap wing protein
-
-
-
-
general phosphoprotein phosphatase
-
-
glycogen synthase phosphatase
-
-
HMG-CoA reductase phosphatase
-
-
-
-
Magnesium-dependent calcium inhibitable phosphatase
-
-
-
-
Microtubule star protein
-
-
-
-
phosphoprotein phosphatase
-
-
-
-
phosphopyruvate dehydrogenase phosphatase
-
-
-
-
phosphospectrin phosphatase
-
-
-
-
polycation modulated (PCM-) phosphatase
-
-
-
-
protein D phosphatase
-
-
-
-
protein phosphatase
-
-
-
-
Protein phosphatase 1A
-
-
-
-
Protein phosphatase 1B
-
-
-
-
Protein phosphatase 1C
-
-
-
-
Protein phosphatase magnesium-dependent 1 delta
-
-
-
-
Protein phosphatase magnesium-dependent 1 gamma
-
-
-
-
Protein phosphatase with EF calcium-binding domain
-
-
-
-
Retinal degeneration C protein
-
-
-
-
Suppressor protein SDS21
-
-
-
-
additional information
calcineurin is a member of the Ser/Thr protein phosphatase family
Pp4
-
-
-
-
PP6
-
-
-
-
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
phosphoprotein + H2O
protein + phosphate
-
-
-
?
phosphorylated histone + H2O
histone + phosphate
-
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
-
-
-
-
?
beta-naphthyl phosphate + H2O
beta-naphthol + phosphate
-
-
-
-
?
cAMP dependent protein kinase + H2O
?
-
-
-
-
?
DLDVPIPGRFDRRVS(P)VAAE + H2O
DLDVPIPGRFDRRVSVAAE + phosphate
-
-
-
-
?
DLDVPIPGRFDRRVY(P)VAAE + H2O
DLDVPIPGRFDRRVYVAAE + phosphate
-
-
-
-
?
DRRVS(P)VAAE + H2O
DRRVSVAAE + phosphate
-
-
-
-
?
DRVY(P)IHPFHL + H2O
DRVYIHPFHL + phosphate
-
-
-
-
?
FDRRVS(P)VAAE + H2O
FDRRVSVAAE + phosphate
-
-
-
-
?
fluorotyrosine phosphate + H2O
fluorotyrosine + phosphate
-
-
-
-
?
FYDEEVDEMY(P)REAPIDKKGNFNY(P)VEFTR + H2O
FYDEEVDEMYREAPIDKKGNFNYVEFTR + phosphate
-
-
-
-
?
glycogen synthase D + H2O
glycogen synthase I + phosphate
histone + H2O
histone + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
phospho-D,L-tyrosine + H2O
D,L-tyrosine + phosphate
-
-
-
-
?
phospho-L-tyrosine + H2O
L-tyrosine + phosphate
-
-
-
-
?
phosphoangiotensin + H2O
angiotensin + phosphate
-
phosphorylated at Tyr
-
-
?
phosphocasein + H2O
casein + phosphate
-
-
-
-
?
phosphohistone + H2O
histone + phosphate
-
-
-
-
?
phosphomyosin light chain + H2O
myosin light chain + phosphate
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
phosphoproteins + H2O
proteins + phosphate
phosphorylase a + H2O
phosphorylase b + phosphate
phosphorylated BCKDH complex + H2O
BCKDH complex + phosphate
-
i.e. branched-chain alpha-keto acid dehydrogenase, phosphorylated
-
-
?
phosphorylated cAMP-dependent protein kinase + H2O
cAMP-dependent protein kinase + phosphate
-
phosphorylated at Ser-95
-
-
?
phosphorylated casein (PKA) + H2O
casein (PKA) + phosphate
-
-
-
-
?
phosphorylated histone + H2O
histone + phosphate
-
-
-
-
?
phosphorylated histone h1 (PKA) + H2O
histone h1 (PKA) + phosphate
-
-
-
-
?
phosphorylated histone h1 (PKC) + H2O
histone h1 (PKC) + phosphate
-
-
-
-
?
phosphorylated myosin light chain peptide + H2O
myosin light chain peptide + phosphate
-
KAKTTKKRPQRATSNVFS
-
-
?
phosphorylated phosphorylase kinase + H2O
phosphorylase kinase + phosphate
-
-
-
-
?
phosphorylated pyruvate dehydrogenase + H2O
pyruvate dehydrogenase + phosphate
-
-
-
-
?
phosphorylated troponin + H2O
troponin + phosphate
-
-
-
-
?
PIPGRFDRRVS(P)VAAE + H2O
PIPGRFDRRVSVAAE + phosphate
-
-
-
-
?
synthetic peptides with phosphotyrosyl residues + H2O
synthetic peptides + phosphate
-
-
-
-
?
tetrafluorotyrosine phosphate + H2O
tetrafluorotyrosine + phosphate
-
-
-
-
?
additional information
?
-
glycogen synthase D + H2O
glycogen synthase I + phosphate
-
-
-
-
?
glycogen synthase D + H2O
glycogen synthase I + phosphate
-
-
-
-
r
phosphoprotein + H2O
protein + phosphate
-
-
-
-
?
phosphoprotein + H2O
protein + phosphate
-
modulation of pyruvate dehydrogenase complex
-
-
?
phosphoprotein + H2O
protein + phosphate
-
phosphatase PP3 stimulates the initiation of DNA-synthesis
-
-
?
phosphoproteins + H2O
proteins + phosphate
-
-
134600, 134601, 134613, 134616, 134617, 134621, 134630, 134632, 134650, 134655, 134656, 134659, 134678 -
-
?
phosphoproteins + H2O
proteins + phosphate
-
comparison of amino acid sequence of substrates at phosphorylated sites
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
-
-
-
-
?
phosphorylase a + H2O
phosphorylase b + phosphate
-
-
-
-
r
additional information
?
-
residues at 97-201 are essential for bovine retina CaN subunit A phosphatase activity
-
-
?
additional information
?
-
-
residues at 97-201 are essential for bovine retina CaN subunit A phosphatase activity
-
-
?
additional information
?
-
-
no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5
-
-
?
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3-hydroxy-3-methylglutaryl CoA
-
-
ADP
-
1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 12% with phosphorylase a
AMP
-
1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 10% with phosphorylase a
bovine serum albumin
-
1 mg/ml 30°C, pH 7.5, 95% remaining activity with glycogen synthase D and 92% with phosphorylase, in presence of 10 mg/ml 79% remaining activity with phosphorylase a as substrate
-
Ca2+
-
degree of inhibition depends on concentration of Mg2+
CaCl2
-
14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 50% remaining activity
cAMP
-
1 mM, 30°C, pH 7.5, 83% remaining activity with histone as substrate and 83% with phosphorylase a
citrate
-
1.4 mM, 30°C, pH 7.5, 68% remaining activity with glycogen synthase D as substrate
EDTA
-
14 mM, 30°C, pH 7.5, 23% remaining activity with glycogen synthase D as substrate and 35% with phosphorylase a
EGTA
-
14 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 83% with phosphorylase a
gelatin
-
1 mg/ml, 30°C, pH 7.5, 92% remaining activity with histone as substrate, and 31% withe phosphorylase a, in presence of 10 mg/ml 10% remaining activity with phohsphorylase a
-
guanosine 5'-(beta,gamma-imido)triphosphate
-
-
histone
-
competitive when glycogen synthase D is the substrate, increases the Km-value for glycogen synthase D 4fold, but shows mixed type inhibition when phosphorylase a acts as substrate with an 1.4fold increase of Km
Inhibitor protein
-
MW 33000-36000, heat and acid stable, specific for BCKDH phosphatase
-
MgCl2
-
14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 60% remaining activity
microcystin-LR
-
inhibition of phosphatase PP3
MnCl2
-
14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 51% remaining activity
myosin light chain hydroxy peptide
-
competitive inhibition
-
Na2HPO4
-
14 mM, 30°C, pH 7.5, no activity with glycogen synthase D as substrate, remaining activity 79% with histone as substrate, and 64% with phosphorylase a as substrate
Na2P2O7
-
14 mM, 30°C, pH 7.5, remaining activity 6% with glycogen synthase D as substrate, 15% with histone as substrate, and 8% with phosphorylase a as substrate
Na2SO4
-
14 mM, 30°C, pH 7.5, remaining activity 53% with glycogen synthase D as substrate and 32% with phosphorylase a as substrate
NaCl
-
14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 83% remaining activity
NaF
-
14 mM, 30°C, pH 7.5, remaining activity 31% with glycogen synthase D as substrate, 67% with histone as substrate, and 37% with phosphorylase a as substrate
NaHCO3
-
14 mM, 30°C, pH 7.5, remaining activity 49% with glycogen synthase D as substrate, 79% with histone as substrate, and 64% with phosphorylase a as substrate
NaNO3
-
14 mM, 30°C, pH 7.5, remaining activity 70% with glycogen synthase D as substrate, 83% with histone as substrate, and 78% with phosphorylase a as substrate
Nucleoside mono-, di-, and triphosphates
-
e.g. GTP, GDP, ATP, ADP
-
phosphorylase a
-
mixed type inhibitor with glycogen synthase d as substrate, increases Km 2fold with only small decearse in vmax
protamine
-
inhibition of phosphatase PP3
rabbit liver glycogen
-
1 mg/ml 30°C, pH 7.5, 88% remaining activity with glycogen synthase D and 37% remaining activity with 10 mg/ml inhibitor
-
UDP
-
1.4 mM, 30°C, pH 7.5, 52% remaining activity with glycogen synthase D as substrate and 20% with phosphorylase a
UMP
-
1.4 mM, 30°C, pH 7.5, 58% remaining activity with glycogen synthase D as substrate and 16% with phosphorylase a
UTP
-
1.4 mM, 30°C, pH 7.5, 44% remaining activity with glycogen synthase D as substrate and 21% with phosphorylase a
ATP
-
-
ATP
-
1.4 mM, 30°C, pH 7.5, 47% remaining activity with glycogen synthase D as substrate and 15% with phosphorylase a
okadaic acid
-
inhibition of phosphatase PP3
okadaic acid
-
no inhibition of phosphatase PP2C
phosphate
-
-
phosphate
-
competitive inhibition to C-subunit and AC-dimer, noncompetitive inhibition to AC-R2
additional information
the residues at 407-456 also have an inhibitory effect on CaN A phosphatase activity in addition to the previously known autoinhibitory domain at 457-480
-
additional information
-
the residues at 407-456 also have an inhibitory effect on CaN A phosphatase activity in addition to the previously known autoinhibitory domain at 457-480
-
additional information
-
study of effects of D-glucose 6-phosphate on inhibitory compounds
-
additional information
-
study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate
-
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ADP
-
1.4 mM, 30°C, pH 7.5, 104% relative activity with histone as substrate
AMP
-
1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate
ATP
-
1.4 mM, 30°C, pH 7.5, 107% relative activity with histone as substrate
bovine serum albumin
-
10 mg/ml, 30°C, pH 7.5, 112% relative activity with histone as substrate
-
CaCl2
-
14 mM, 30°C, pH 7.5, relative activity 126% with glycogen synthase D as substrate and 232% with histone as substrate compared to no addition
cAMP
-
1 mM, 30°C, pH 7.5, 115% relative activity with glycogen synthase D as substrate
D-glucosamine 6-phosphate
-
1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 117% with histone as substrate, and 102% with phosphorylase a as substrate compared to no addition
D-glucose
-
1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 101% with histone as substrate, and 106% with phosphorylase a as substrate compared to no addition
D-glucose 6-phosphate
-
1.4 mM, 30°C, pH 7.5, 109% relative activity with phosphorylase a as substrate
EDTA
-
14 mM, 30°C, pH 7.5, 175% relative activity with histone as substrate
EGTA
-
14 mM, 30°C, pH 7.5, 127% relative activity with histone as substrate
gelatin
-
1 mg/ml, 30°C, pH 7.5, 128% relative activity with glycogen synthase D as substrate, with 10 mg/ml 110% relative activity
-
Inhibitor-2
-
stimulation of phosphatase PP3
-
MgCl2
-
14 mM, 30°C, pH 7.5, relative activity 256% with glycogen synthase D as substrate and 238% with histone as substrate compared to no addition
MnCl2
-
14 mM, 30°C, pH 7.5, relative activity 361% with glycogen synthase D as substrate and 280% with histone as substrate compared to no addition
Na2SO4
-
14 mM, 30°C, pH 7.5, relative activity 152% with histone as substrate compared to no addition
NaCl
-
14 mM, 30°C, pH 7.5, relative activity 102% with glycogen synthase D as substrate and 108% with histone as substrate compared to no addition
rabbit liver glycogen
-
1 mg/ml, 30°C, pH 7.5, 119% relative activity with histone as substrate
-
UMP
-
1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate
UTP
-
1.4 mM, 30°C, pH 7.5, 108% relative activity with histone as substrate
additional information
-
regulated by phosphorylation, phosphatase 2B
-
additional information
-
study of effects of D-glucose 6-phosphate on activating compounds
-
additional information
-
study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate
-
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Nakai, C.; Thomas, J.A.
Substrate specificity of glycogen synthase phosphatase from bovine heart: action on phosphorylase a and histone
Biochem. Biophys. Res. Commun.
52
530-536
1973
Bos taurus
brenda
Blumenthal, D.K.; Chan, C.P.; Takio, K.; Gallis, B.; Hansen, R.S.; Krebs, E.G.
Substrate specificity of calmodulin-dependent protein phosphatases
Adv. Protein Phosphatases
1
163-174
1985
Bos taurus
-
brenda
Simonelli, P.F.; Li, H.G.
Regulation of calmodulin-dependent phosphoprotein phosphatase (calcineurin) by synergistic action of Ca(II), calmodulin, and Mg(II) or transition metal ions
Adv. Protein Phosphatases
1
175-190
1985
Bos taurus
-
brenda
Mumby, M.
The structure and function of cardiac protein phosphatases
Adv. Protein Phosphatases
3
301-326
1986
Bos taurus, Canis lupus familiaris, Oryctolagus cuniculus, Mammalia, Rattus norvegicus, Sus scrofa
-
brenda
Reed, L.J.; Damuni, Z.
Mitochondrial protein phosphatases
Adv. Protein Phosphatases
4
59-76
1987
Bos taurus
-
brenda
Damuni, Z.; Reed, L.J.
Purification and characterization of a divalent cation-independent, spermine-stimulated protein phosphatase from bovine kidney mitochondria
J. Biol. Chem.
262
5133-5138
1987
Bos taurus
brenda
Martin, B.L.; Graves, D.J.
The chemical mechanism of calcineurin: A model for phosphotyrosyl protein phosphatases
Adv. Protein Phosphatases
4
95-125
1987
Bos taurus
-
brenda
Stone, S.R.; Hofsteenge, J.; Hemmings, B.A.
The primary structure of the catalytic subunit of phosphatase 2A as determined by molecular cloning: conservation of sequence between species and existence of isotypes
Adv. Protein Phosphatases
4
375-389
1987
Bos taurus, Oryctolagus cuniculus, Sus scrofa
-
brenda
Green, D.D.; Yang, S.; Mumby, M.C.
Molecular cloning and sequence analysis of the catalytic subunit of bovine type 2A protein phosphatase
Proc. Natl. Acad. Sci. USA
84
4880-4884
1987
Bos taurus
brenda
Merat, D.L.; Cheung, W.Y.
Calmodulin-dependent protein phosphatase: isolation of subunits and reconstitution to holoenzyme
Methods Enzymol.
139
79-87
1987
Bos taurus
brenda
Chernoff, J.; Seels, M.A.; Li, H.C.
Characterization of phosphotyrosyl-protein phosphatase activity associated with calcineurin
Biochem. Biophys. Res. Commun.
121
141-148
1984
Bos taurus
brenda
Damuni, Z.; Reed, L.J.
Purification and properties of the catalytic subunit of the branched-chain alpha-keto acid dehydrogenase phosphatase from bovine kidney mitochondria
J. Biol. Chem.
262
5129-5132
1987
Bos taurus
brenda
King, M.M.; Huang, C.Y.
The calmodulin-dependent activation and deactivation of the phosphoprotein phosphatase, calcineurin, and the effect of nucleotides, pyrophosphate, and divalent metal ions. Identification of calcineurin as a Zn and Fe metalloenzyme
J. Biol. Chem.
259
8847-8856
1984
Bos taurus
brenda
Honkanen, R.E.; Zwiller, J.; Daily, S.L.; Khatra, B.S.; Dukelow, M.; Boynton, A.L.
Identification, purification, and characterization of a novel serine/threonine protein phosphatase from bovine brain
J. Biol. Chem.
266
6614-6619
1991
Bos taurus
brenda
Klumpp, S.; Selke, D.; Fischer, D.; Baumann, A.; Muller, F.; Thanos, S.
Protein phosphatase type-2C isozymes present in vertebrate retinae: purification, characterization, and localization in photoreceptors
J. Neurosci. Res.
51
328-338
1998
Bos taurus
brenda
Price, N.E.; Mumby, M.C.
Effects of regulatory subunits on the kinetics of protein phosphatase 2A
Biochemistry
39
11312-11318
2000
Bos taurus
brenda
Tolstykh, T.; Lee, J.; Vafai, S.; Stock, J.B.
Carboxyl methylation regulates phosphoprotein phosphatase 2A by controlling the association of regulatory B subunits
EMBO J.
19
5682-5691
2000
Bos taurus
brenda
Nakai, C.; Thomas, J.A.
Properties of a phosphoprotein phosphatase from bovine heart with activity on glycogen synthase, phosphorylase, and histone
J. Biol. Chem.
249
6459-6467
1974
Bos taurus
brenda
Kloeker, S.; Reed, R.; McConnell, J.L.; Chang, D.; Tran, K.; Westphal, R.S.; Law, B.K.; Colbran, R.J.; Kamoun, M.; Campbell, K.S.; Wadzinski, B.E.
Parallel purification of three catalytic subunits of the protein serine/threonine phosphatase 2A family (PP2A(C), PP4(C), and PP6(C)) and analysis of the interaction of PP2A(C) with alpha4 protein
Protein Expr. Purif.
31
19-33
2003
Bos taurus, Mus musculus, Rattus norvegicus
brenda
Zuo, Y.; Selvakumar, P.; Sharma, R.K.
Molecular cloning and biochemical characterization of bovine retina calcineurin
Mol. Cell. Biochem.
333
73-82
2010
Bos taurus (P48452), Bos taurus
brenda
Zhang, M.; Yogesha, S.D.; Mayfield, J.E.; Gill, G.N.; Zhang, Y.
Viewing serine/threonine protein phosphatases through the eyes of drug designers
FEBS J.
280
4739-4760
2013
Homo sapiens (P36873), Bos taurus (P48452)
brenda