Information on EC 3.1.1.10 - tropinesterase

for references in articles please use BRENDA:EC3.1.1.10
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.1.10
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RECOMMENDED NAME
GeneOntology No.
tropinesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
atropine + H2O = tropine + tropate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
-
hydrolysis of carboxylic ester
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tropane, piperidine and pyridine alkaloid biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
atropine acylhydrolase
Also acts on cocaine and other tropine esters.
CAS REGISTRY NUMBER
COMMENTARY hide
59536-71-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
PMBL-1
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-
Manually annotated by BRENDA team
AT3
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-
Manually annotated by BRENDA team
AT3
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-hyoscyamine + H2O
tropine + tropic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
atropine + H2O
3-hydroxy-2-phenylpropanoic acid
show the reaction diagram
-
-
-
-
?
atropine + H2O
tropine + tropate
show the reaction diagram
butanilicain + H2O
?
show the reaction diagram
-
i.e. 2-butylamino-N-(2-chloro-6-methylphenyl)acetamide
-
-
?
methylbutyrate + H2O
methanol + butanoate
show the reaction diagram
-
-
-
-
?
N(1,1-dimethyl-2-ureidoethyl)-2-hydroxy-3-(o-fluorobenzoyloxy)propylamine + H2O
?
show the reaction diagram
-
i.e. flestolol
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
atropine + H2O
tropine + tropate
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
atropine
-
competitive inhibition of hydrolysis of butyrylcholine
phosphoric acid-bis-4-nitrophenyl ester
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012 - 0.05
atropine
1.9 - 16
Butyrylcholine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
68
atropine
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-
1930
Butyrylcholine
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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hydrolysis of methylbutyrate
7 - 8.5
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hydrolysis of butyrylcholine
8 - 8.5
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hydrolysis of atropine
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
-
gel filtration
57200
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low speed sedimentation
57800
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equilibrium sedimentation
61000
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1 * 61000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 61000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (417 entries)
Please use the Sequence Search for a specific query.