Information on EC 2.8.3.10 - citrate CoA-transferase

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The expected taxonomic range for this enzyme is: Klebsiella aerogenes

EC NUMBER
COMMENTARY hide
2.8.3.10
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RECOMMENDED NAME
GeneOntology No.
citrate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + citrate = acetate + (3S)-citryl-CoA
show the reaction diagram
the enzyme is a component of EC 4.1.3.6 [citrate(pro-3-S)-lyase]. Also catalyses the transfer of thioacyl carrier protein from its acetyl thioester to citrate
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
citrate degradation
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:citrate CoA-transferase
The enzyme is a component of EC 4.1.3.6 [citrate (pro-3S)-lyase]. Also catalyses the transfer of thioacyl carrier protein from its acetyl thioester to citrate.
CAS REGISTRY NUMBER
COMMENTARY hide
65187-14-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 13882
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + citrate
acetate + (3S)-citryl-CoA
show the reaction diagram
acetyl-dephospho-CoA + citrate
acetate + (3S)-citryl-dephospho-CoA
show the reaction diagram
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r
acetyl-[acyl-carrier protein] + citrate
acetate + (3S)-citryl-[acyl-carrier protein]
show the reaction diagram
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r
butyryl-CoA + citrate
butyrate + (3S)-citryl-CoA
show the reaction diagram
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r
propionyl-CoA + citrate
propionate + (3S)-citryl-CoA
show the reaction diagram
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r
additional information
?
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also catalyzes a citrate independent exchange reaction of acetyl residues between acetyl-thioacyl carrier protein or acetyl-CoA and acetate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + citrate
acetate + (3S)-citryl-CoA
show the reaction diagram
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biologically significant reaction of transferase subunit as part of citrate lyase, the isolated transferase subunit represents not only an acetyl-thioacyl carrier protein, citrate acyl carrier protein transferase but also an acetyl-CoA:citrate CoA-transferase
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r
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
(3S)-citryl-CoA
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pH 8.0, 25C
1.3 - 6.7
acetyl-CoA
1.3 - 12.5
acetyl-dephospho-CoA
1.3
butyryl-CoA
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pH 8.0, 25C, isolated transferase
1.3 - 3.3
propionyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26.7
(3S)-citryl-CoA
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pH 8.0, 25C
21.7
acetyl-CoA
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pH 8.0, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.1
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acetyl-CoA, integrated into enzyme complex
25
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acetyl-CoA, isolated transferase subunit
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complete EC 4.1.3.6 citrate lyase with 3 subunits, EC 2.8.3.10 is the largest subunit
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