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Information on EC 2.8.2.8 - [heparan sulfate]-glucosamine N-sulfotransferase and Organism(s) Bos taurus and UniProt Accession O97583

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IUBMB Comments
The enzyme also catalyses the sulfation of chondroitin 4-sulfate and dermatan sulfate, but to a much more limited extent.
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This record set is specific for:
Bos taurus
UNIPROT: O97583
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The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ndst1, ndst-1, ndst2, ndst3, ndst-2, ndst4, n-deacetylase/n-sulfotransferase-1, n-deacetylase/n-sulfotransferase, n-deacetylase/n-sulfotransferase 1, heparan sulfate n-deacetylase/n-sulfotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heparin/heparan sulfate N-deacetylase/N-sulfotransferase
-
N-acetylglucosaminyl N-deacetylase/N-sulfotransferase
-
3'-phosphoadenylyl-sulfate:heparitin N-sulfotransferase
-
-
-
-
3'-phosphoadenylylsulfate:N-desulfoheparin N-sulfotransferase
-
-
-
-
3'-phosphoadenylylsulfate:N-desulfoheparin sulfotransferase
-
-
-
-
desulfoheparin sulfotransferase
-
-
-
-
heparan sulfate 2-N-sulfotransferase
-
-
-
-
heparan sulfate N-deacetylase/N-sulfotransferase
-
-
-
-
heparan sulfate N-sulfotransferase
-
-
-
-
heparan sulfate sulfotransferase
-
-
-
-
heparin N-sulfotransferase
-
-
-
-
heparitin sulfotransferase
-
-
-
-
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3
-
-
N-desulfoheparin sulfotransferase
-
-
-
-
N-heparan sulfate sulfotransferase
-
-
-
-
N-HSST
-
-
-
-
NDST3
-
-
PAPS:DSH sulfotransferase
-
-
-
-
PAPS:N-desulfoheparin sulfotransferase
-
-
-
-
sulfotransferase, desulfoheparin
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine N-sulfotransferase
The enzyme also catalyses the sulfation of chondroitin 4-sulfate and dermatan sulfate, but to a much more limited extent.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-75-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + N-deacetylase K5-polysaccharide
?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
show the reaction diagram
3'-phosphoadenylylsulfate + heparin
adenosine 3',5'-bisphosphate + N-sulfoheparin
show the reaction diagram
-
-
?
3'-phosphoadenylylsulfate + N-deacetylated K5-polysaccharide
?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-D-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfo-D-glucosamine
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + heparin
adenosine 3',5'-bisphosphate + N-sulfoheparin
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N,O-desulfated heparan sulfate
adenosine 3',5'-bisphosphate + O-desulfated heparan sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N,O-desulfoheparan sulfate tetrasaccharide
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N,O-desulfoheparan sulfate tetrasaccharides with the nonreducing terminus occupied by glucuronic acid
?
show the reaction diagram
3'-phosphoadenylylsulfate + N-desulfated heparan sulfate
adenosine 3',5'-bisphosphate + heparan sulfate
show the reaction diagram
-
chemically de-N-sulfated heparan sulfate is a better substrate than heparan sulfate
-
?
3'-phosphoadenylylsulfate + N-desulfoheparan sulfate
adenosine 3',5'-bisphosphate + heparan sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + oligosaccharides derived from N-desulfoheparan sulfate
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
poor acceptors: N-desulfo-N-acetylheparan, heparin, N-desulfoheparin
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
show the reaction diagram
3'-phosphoadenylyl sulfate + [heparan sulfate]-D-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfo-D-glucosamine
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
41% of the activity with Mn2+
Mg2+
-
52% of the activity with Mn2+
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0173
3'-phosphoadenylylsulfate
-
0.0087
N-deacetylase K5-polysaccharide
-
-
2.5
N,O-desulfoheparan sulfate tetrasaccharide
-
calculated from disaccharide units
-
1.89
N-desulfoheparan sulfate
-
calculated from disaccharide units
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional enzyme possessing heparan sulfate N-deacetylase and N-sulfotransferase activity
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
trachea, cell culture
Manually annotated by BRENDA team
embryonic, cell culture
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NDST2_BOVIN
884
1
100897
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
peaks with enzyme activity: 200000 and 110000 Da
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
N-acetylglucosaminyl N-deacetylase/N-sulfotransferase, recombinant from COS-1 cells, 1-step affinity chromatographical purification
N-desulfo-N-acetylheparan sulfate deacetylase activity copurifies
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
lambdagt10 library construction and screening, DNA sequence determination and analysis, expression in COS-1 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ghler, D.; Niemann, R.; Buddecke, E.
Purification and characterization of 3-phosphoadenylylsulfate: N-desulfoheparan sulfate sulfotransferase from arterial tissue
Eur. J. Biochem.
138
301-308
1984
Bos taurus
Manually annotated by BRENDA team
Foley, T.; Baker, J.R.
A heparan sulphate sulphotransferase from ox lung
Biochem. J.
124
25P-26P
1971
Bos taurus
Manually annotated by BRENDA team
Toma, L.; Berninsone, P.; Hirschberg, C.B.
The putative heparin-specific N-acetylglucosaminyl N-Deacetylase/N-sulfotransferase also occurs in non-heparin-producing cells
J. Biol. Chem.
273
22458-22465
1998
Bos taurus (O97583), Bos taurus
Manually annotated by BRENDA team
Jiang, Z.; Michal, J.J.; Wu, X.L.; Pan, Z.; MacNeil, M.D.
The heparan and heparin metabolism pathway is involved in regulation of fatty acid composition
Int. J. Biol. Sci.
7
659-663
2011
Bos taurus
Manually annotated by BRENDA team