Information on EC 2.7.99.1 - triphosphate-protein phosphotransferase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
2.7.99.1
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RECOMMENDED NAME
GeneOntology No.
triphosphate-protein phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
triphosphate + [microsomal-membrane protein] = diphosphate + phospho-[microsomal-membrane protein]
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
triphosphate:[microsomal-membrane-protein] phosphotransferase
This enzyme was originally thought to use diphosphate as substrate [1] but this has since been disproved [2]. The activity is observed as the second part of a biphasic reaction after depletion of ATP. Tripolyphosphate is a contaminant of [gamma-32P]ATP.
CAS REGISTRY NUMBER
COMMENTARY hide
74092-32-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diphosphate + microsomal polypeptides
phosphate + phosphorylated microsomal polypeptides
show the reaction diagram
tetraphosphate + [microsomal membrane protein]
triphosphate + phosphorylated [microsomal membrane protein]
show the reaction diagram
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reversible phosphorylation, substrates are 145 kD and 130 kD microsomal membrane proteins, higher activity compared to triphosphate as phosphate donor
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-
?
triphosphate + [microsomal membrane protein]
diphosphate + phosphorylated [microsomal membrane protein]
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + microsomal polypeptides
phosphate + phosphorylated microsomal polypeptides
show the reaction diagram
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?
triphosphate + [microsomal membrane protein]
diphosphate + phosphorylated [microsomal membrane protein]
show the reaction diagram
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reversible phosphorylation in the second phase of membrane protein phosphorylation, competes with ATP-dependent phosphorylation reaction which occurs in the first phase
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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micromolar levels required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-adenylimidodiphosphate
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i.e. AMP-PNP, ATP analogue
adenosine 5'-(beta,gamma-Imino)triphosphate
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i.e. AMP-PNP, ATP analogue
adenosine 5'-(beta,gamma-methylene)triphosphate
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i.e. AMP-PCP or AMP-CPP, ATP analogue
adenosine 5'-methylene diphosphate
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i.e. AMP-CP, ATP analogue
Mg2+
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1 mM, strong
NaF
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5 mM, strong
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from liver microsomal membranes by DEAE ion exchange chromatography and gel filtration
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