Information on EC 2.7.1.B6 - tagatose-1-phosphate kinase

for references in articles please use BRENDA:EC2.7.1.B6
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.B6
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
tagatose-1-phosphate kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-tagatose 1-phosphate = ADP + D-tagatose 1,6-bisphosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-tagatose-1-phosphate 6-phosphotransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
annotated as fructose-1-phosphate kinase; gene tagK or BL01904
UniProt
Manually annotated by BRENDA team
annotated as fructose-1-phosphate kinase; gene tagK or BL01904
UniProt
Manually annotated by BRENDA team
annotated as 1-phosphofructokinase; gene tagK
UniProt
Manually annotated by BRENDA team
annotated as 1-phosphofructokinase; gene tagK
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-tagatose 1-phosphate
ADP + D-tagatose 1,6-bisphosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-tagatose 1-phosphate
ADP + D-tagatose 1,6-bisphosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose-6-phosphate
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D-tagatose 6-phosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Michaelis-Menten kinetics, the catalytic efficiency of the enzyme for D-tagatose 1-phosphate of 137.5 M/s is 40fold higher than that for fructose 1-phosphate with 3.4 M/s, confirming that the enzyme is a D-tagatose 1-phosphate-specific kinase
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) b nickel affinity chromatography and desalting gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene tagK, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha