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Information on EC 2.5.1.9 - riboflavin synthase and Organism(s) Brucella abortus and UniProt Accession Q2YN92

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IUBMB Comments
A flavoprotein (riboflavin).
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This record set is specific for:
Brucella abortus
UNIPROT: Q2YN92
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The taxonomic range for the selected organisms is: Brucella abortus
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
riboflavin synthase, riboflavin synthetase, heavy riboflavin synthase, light riboflavin synthase, lumazine synthase/riboflavin synthase complex, riboflavine synthetase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
light riboflavin synthase
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riboflavin synthetase
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riboflavine synthase
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riboflavine synthetase
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synthase, riboflavin
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dismutation
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SYSTEMATIC NAME
IUBMB Comments
6,7-dimethyl-8-(1-D-ribityl)lumazine:6,7-dimethyl-8-(1-D-ribityl)lumazine 2,3-butanediyltransferase
A flavoprotein (riboflavin).
CAS REGISTRY NUMBER
COMMENTARY hide
9075-82-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6,7-dimethyl-8-(1-D-ribityl)lumazine
riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
show the reaction diagram
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?
2 6,7-dimethyl-8-(1-D-ribityl)lumazine
riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6,7-dimethyl-8-(1-D-ribityl)lumazine
riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
show the reaction diagram
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-
-
?
2 6,7-dimethyl-8-(1-D-ribityl)lumazine
riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
show the reaction diagram
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-
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?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2-ethyl-5-oxo-5H-[1,3,4]thiadiazolo[3,2-a]pyrimidin-7-yl)methyl 3,4,5-triethoxybenzoate
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(2E)-3-(2,3-dimethoxyphenyl)-N-[4-methyl-2-(4-methylpiperazin-1-yl)quinolin-6-yl]prop-2-enamide
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2-(4-ethoxyphenyl)-N-[3-(piperidin-1-yl)propyl]imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
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2-(4-methoxyphenyl)-N-[3-(piperidin-1-yl)propyl]imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
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2-[ethyl(methyl)amino]ethyl 4-[(5-ethyl-4-oxo-4,5-dihydrothieno[3,2-c]quinoline-2-carbonyl)amino]benzoate
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5-[3-(propan-2-yl)-1H-pyrazol-5-yl]-3-(3,4,5-trimethoxyphenyl)-1,2,4-oxadiazole
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ethyl 5-(diethylcarbamoyl)-2-[2-(4-methoxyphenoxy)acetamido]-4-methylthiophene-3-carboxylate
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N-[3-(azepan-1-yl)propyl]-2-(4-methoxyphenyl)imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
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N-[3-(diethylamino)propyl]-2-(4-ethoxyphenyl)imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
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N-[3-(diethylamino)propyl]-2-(4-methylphenyl)imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
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additional information
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a high-throughput screening for inhibitors of riboflavin synthase identifies antimicrobial compounds to treat brucellosis
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0024
6,7-dimethyl-8-(1-D-ribityl)lumazine
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pH 7.5, 37°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02964
(2-ethyl-5-oxo-5H-[1,3,4]thiadiazolo[3,2-a]pyrimidin-7-yl)methyl 3,4,5-triethoxybenzoate
Brucella abortus
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pH 7.5, 37°C
0.01915
(2E)-3-(2,3-dimethoxyphenyl)-N-[4-methyl-2-(4-methylpiperazin-1-yl)quinolin-6-yl]prop-2-enamide
Brucella abortus
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pH 7.5, 37°C
0.02564
2-(4-ethoxyphenyl)-N-[3-(piperidin-1-yl)propyl]imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
Brucella abortus
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pH 7.5, 37°C
0.03118
2-(4-methoxyphenyl)-N-[3-(piperidin-1-yl)propyl]imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
Brucella abortus
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pH 7.5, 37°C
0.00235
2-[ethyl(methyl)amino]ethyl 4-[(5-ethyl-4-oxo-4,5-dihydrothieno[3,2-c]quinoline-2-carbonyl)amino]benzoate
Brucella abortus
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pH 7.5, 37°C
0.03053
5-[3-(propan-2-yl)-1H-pyrazol-5-yl]-3-(3,4,5-trimethoxyphenyl)-1,2,4-oxadiazole
Brucella abortus
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pH 7.5, 37°C
0.01912
ethyl 5-(diethylcarbamoyl)-2-[2-(4-methoxyphenoxy)acetamido]-4-methylthiophene-3-carboxylate
Brucella abortus
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pH 7.5, 37°C
0.02638
N-[3-(azepan-1-yl)propyl]-2-(4-methoxyphenyl)imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
Brucella abortus
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pH 7.5, 37°C
0.0278
N-[3-(diethylamino)propyl]-2-(4-ethoxyphenyl)imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
Brucella abortus
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pH 7.5, 37°C
0.03187
N-[3-(diethylamino)propyl]-2-(4-methylphenyl)imidazo[2,1-b][1,3]benzothiazole-7-carboxamide
Brucella abortus
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pH 7.5, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug target
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the enzyme from Brucella abortus constitutes a suitable target for alternative antibacterial therapy, notably against strains resistant to conventional antibiotic treatments. A high-throughput screening for inhibitors of riboflavin synthase identifies antimicrobial compounds to treat brucellosis
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotrimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 12% (w/v) PEG 8000, 10% (w/v) glycerol, 0.5 M potassium chloride
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Superdex 200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli BL21(DE3) cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Serer, M.I.; Bonomi, H.R.; Guimaraes, B.G.; Rossi, R.C.; Goldbaum, F.A.; Klinke, S.
Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility
Acta Crystallogr. Sect. D
70
1419-1434
2014
Brucella abortus (Q2YN92), Brucella abortus, Brucella abortus 2308 (Q2YN92)
Manually annotated by BRENDA team
Serer, M.I.; Carrica, M.D.C.; Trappe, J.; Lopez Romero, S.; Bonomi, H.R.; Klinke, S.; Cerutti, M.L.; Goldbaum, F.A.
A high-throughput screening for inhibitors of riboflavin synthase identifies novel antimicrobial compounds to treat brucellosis
FEBS J.
286
2522-2535
2019
Brucella abortus
Manually annotated by BRENDA team