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Information on EC 2.4.2.48 - tRNA-guanine15 transglycosylase and Organism(s) Thermoplasma acidophilum and UniProt Accession Q9HI54

for references in articles please use BRENDA:EC2.4.2.48
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.48 tRNA-guanine15 transglycosylase
IUBMB Comments
Archaeal tRNAs contain the modified nucleoside archaeosine at position 15. This archaeal enzyme catalyses the exchange of guanine at position 15 of tRNA with the base preQ0, which is ultimately modified to form the nucleoside archaeosine (cf. EC 2.6.1.97) .
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This record set is specific for:
Thermoplasma acidophilum
UNIPROT: Q9HI54
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The taxonomic range for the selected organisms is: Thermoplasma acidophilum
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Reaction Schemes
hide
guanine15 in tRNA
+
7-cyano-7-carbaguanine
=
7-cyano-7-carbaguanine15 in tRNA
+
guanine
guanine15 in tRNA
+
=
7-cyano-7-carbaguanine15 in tRNA
+
Synonyms
archaeosine trna-guanine transglycosylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
multisite-specific archaeosine tRNA-guanine transglycosylase
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archaeosine tRNA-guanine transglycosylase
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transfer ribonucleic acid guanine15 transglycosylase
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tRNA guanine15 transglycosidase
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
tRNA-guanine15:7-cyano-7-carbaguanine tRNA-D-ribosyltransferase
Archaeal tRNAs contain the modified nucleoside archaeosine at position 15. This archaeal enzyme catalyses the exchange of guanine at position 15 of tRNA with the base preQ0, which is ultimately modified to form the nucleoside archaeosine (cf. EC 2.6.1.97) [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
guanine13 in tRNA(Leu) + 7-cyano-7-carbaguanine
7-cyano-7-carbaguanine13 in tRNA(Leu) + guanine
show the reaction diagram
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-
-
?
guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine
7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine
show the reaction diagram
-
-
-
?
guanine15 in tRNA + 7-cyano-7-deazaguanine
7-cyano-7-deazaguanine15 in tRNA + guanine
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guanine15 in tRNA + 7-cyano-7-deazaguanine
7-cyano-7-deazaguanine15 in tRNA + guanine
show the reaction diagram
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-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap Q-Sepharose and HiTrap Heparin-Sepharose column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
because the Thermoplasma acidophilum enzyme can not be expressed as a soluble protein in Escherichia coli, an expression system using another thermophilic archaeon, Thermococcus kodakarensis is employed. The arcTGT gene in Thermococcus kodakarensis is disrupted, complemented with the Thermoplasma acidophilum arcTGT gene, and tRNALeu variants are expressed. Mass spectrometry analysis of purified tRNALeu variants reveals the modifications of archaeosine13 and archaeosine15 in the wild-type tRNALeu
expressed in Thermococcus kodakarensis
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kawamura, T.; Hirata, A.; Ohno, S.; Nomura, Y.; Nagano, T.; Nameki, N.; Yokogawa, T.; Hori, H.
Multisite-specific archaeosine tRNA-guanine transglycosylase (ArcTGT) from Thermoplasma acidophilum, a thermo-acidophilic archaeon
Nucleic Acids Res.
44
1894-1908
2016
Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
Manually annotated by BRENDA team
Kawamura, T.; Hirata, A.; Ohno, S.; Nomura, Y.; Nagano, T.; Nameki, N.; Yokogawa, T.; Hori, H.
Multisite-specific archaeosine tRNA-guanine transglycosylase (ArcTGT) from Thermoplasma acidophilum, a thermo-acidophilic archaeon
Nucleic Acids Res.
44
1894-1908
2015
Pyrococcus horikoshii (O58843), Pyrococcus horikoshii, Pyrococcus horikoshii OT-3 (O58843), Thermoplasma acidophilum (Q9HI54), Thermoplasma acidophilum
Manually annotated by BRENDA team