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2 dTDP-2-deoxy alpha-D-glucose + 2 genistein
2 dTDP + 7-O-(2-deoxy-beta-D-glucopyranosyl)genistein + 4'-O-(2-deoxy-beta-D-glucopyranosyl)genistein
2 UDP-D-glucose + corylifol A
2 UDP + corylifol A-4',7-di-O-beta-D-glucopyranoside
2-nitrophenyl-beta-D-glucopyranoside + chrysin
2-nitrophenol + 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
2-nitrophenyl-beta-D-glucopyranoside + oroxylin A
2-nitrophenol + 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
2-nitrophenyl-beta-D-glucopyranoside + wogonin
2-nitrophenol + 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
ADP-glucose + 7,8-dihydroxyflavone
ADP + 7-O-beta-D-glucosyl-7,8-dihydroxyflavone
CDP-glucose + 4',7-dihydroxyflavone
CDP + 7-O-beta-D-glucosyl-4',7-dihydroxyflavone
dTDP-beta-L-rhamnose + genistein
dTDP + 7-O-(alpha-L-rhamnopyranosyl)genistein
-
-
-
?
TDP-glucose + curcumin
TDP + 7-O-beta-D-glucosyl-curcumin
TDP-glucose + luteolin
TDP + 7-O-beta-D-glucosylluteolin
-
-
-
?
UDP-alpha-D-glucose + 3,7,4'-trihydroxyflavone
UDP + 7-O-beta-D-glucosyl-3,7,4'-trihydroxyflavone
-
9% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + 5,7-dihydroxyflavanone
UDP + 7-O-beta-D-glucosyl-5,7-dihydroxyflavanone
-
15% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + 7,4'-dihydroxyflavone
UDP + 7-O-beta-D-glucosyl-7,4'-dihydroxyflavone
-
20% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + acacetin
UDP + 7-O-beta-D-glucosylacacetin
-
24% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + apigenin
UDP + 7-O-beta-D-glucosylapigenin
-
-
-
?
UDP-alpha-D-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
29.6% of the activity with eriodictyol
-
-
?
UDP-alpha-D-glucose + chrysoeriol
UDP + 7-O-beta-D-glucosylchrysoeriol
-
63% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + datiscetin
UDP + 7-O-beta-D-glucosyldatiscetin
-
9% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + fisetin
UDP + 7-O-beta-D-glucosylfisetin
-
14% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + galangin
UDP + 7-O-beta-D-glucosylgalangin
-
9% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + kaempferid
UDP + 7-O-beta-D-glucosylkaempferid
-
16% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + 7-O-beta-D-glucosylkaempferol
-
11% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
78.9% of the activity with eriodictyol
-
-
?
UDP-alpha-D-glucose + luteolin
UDP + 7-O-beta-D-glucosylluteolin
-
210% of the activity with apigenin
-
?
UDP-alpha-D-glucose + naringenin
UDP + 7-O-beta-D-glucosylnaringenin
-
45% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + 7-O-beta-D-glucosylquercetin
-
16% of the activity with apigenin
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
64.1% of the activity with eriodictyol
-
-
?
UDP-D-glucose + corylifol A
UDP + corylifol A-4'-O-beta-D-glucopyranoside
UDP-galactose + daidzein
UDP + 7-O-beta-D-galactosyldaidzein
-
-
-
-
?
UDP-galactose + genistein
UDP + 7-O-beta-D-galactosylgenistein
-
-
-
-
?
UDP-galactose + myricetin
UDP + 7-O-beta-D-galactosylmyricetin
-
-
-
-
?
UDP-galactose + quercetin
UDP + 7-O-beta-D-galactosylquercetin
-
-
-
-
?
UDP-glucose + 2-chlorocinnamic acid
UDP + 1-O-[2-chlorocinnamoyl]-beta-D-glucopyranose
low activity
-
-
?
UDP-glucose + 2-coumaric acid
UDP + 1-O-[2-coumaroyl]-beta-D-glucopyranose
high activity
-
-
?
UDP-glucose + 2-methylcinnamic acid
UDP + 1-O-[2-methylcinnamoyl]-beta-D-glucopyranose
-
-
-
?
UDP-glucose + 3',4',5,7-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-3',4',5,7-tetrahydroxyflavone
-
involved in biosynthesis of flavone glucosides
-
-
?
UDP-glucose + 3-chlorocinnamic acid
UDP + 1-O-[3-chlorocinnamoyl]-beta-D-glucopyranose
low activity
-
-
?
UDP-glucose + 3-hydroxycoumarin
UDP + ?
UDP-glucose + 4-coumaric acid
UDP + 1-O-[4-coumaroyl]-beta-D-glucopyranose
UDP-glucose + 5,7,3',4'-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
-
-
-
?
UDP-glucose + 7-hydroxyflavone
UDP + 7-O-beta-D-glucosyl-7-hydroxyflavone
UDP-glucose + apigenin
UDP + ?
UDP-glucose + apigenin
UDP + apigenin 7-O-glucoside
-
-
-
-
?
UDP-glucose + baicalein
UDP + ?
UDP-glucose + biochanin A
UDP + ?
UDP-glucose + caffeic acid
UDP + 1-O-caffeoyl-beta-D-glucopyranose
UDP-glucose + chrysin
UDP + ?
UDP-glucose + daidzein
UDP + 7-O-beta-D-glucosyldaidzein
-
-
-
-
?
UDP-glucose + eriodictyol
UDP + 7-O-beta-D-glucosyl-eriodictyol
-
-
-
?
UDP-glucose + ferulic acid
UDP + 1-O-feruolyl-beta-D-glucopyranose
UDP-glucose + genistein
UDP + 4'-O-(beta-D-glucopyranosyl)genistein + 7-O-(beta-D-glucopyranosyl)genistein
UDP-glucose + genistein
UDP + 7-O-(beta-D-glucopyranosyl)genistein
-
-
-
?
UDP-glucose + genistein
UDP + 7-O-beta-D-glucosylgenistein
-
-
-
-
?
UDP-glucose + genistein
UDP + ?
-
-
-
?
UDP-glucose + luteolin
UDP + 7-O-beta-D-glucosyl-luteolin
92.1% of the activity with eriodictyol
-
-
?
UDP-glucose + luteolin
UDP + ?
-
-
-
?
UDP-glucose + myricetin
UDP + 7-O-beta-D-glucosylmyricetin
-
-
-
-
?
UDP-glucose + naringenin
UDP + 7-O-beta-D-glucosyl-naringenin
71.6% of the activity with eriodictyol
-
-
?
UDP-glucose + naringenin
UDP + ?
-
-
-
?
UDP-glucose + oroxylin A
UDP + ?
UDP-glucose + quercetin
UDP + ?
-
-
-
?
UDP-glucose + scutellarein
UDP + ?
UDP-glucose + sinapic acid
UDP + 1-O-sinapoyl-beta-D-glucopyranose
UDP-glucose + vanillic acid
UDP + ?
low activity
-
-
?
UDP-glucose + wogonin
UDP + ?
additional information
?
-
2 dTDP-2-deoxy alpha-D-glucose + 2 genistein

2 dTDP + 7-O-(2-deoxy-beta-D-glucopyranosyl)genistein + 4'-O-(2-deoxy-beta-D-glucopyranosyl)genistein
-
-
-
?
2 dTDP-2-deoxy alpha-D-glucose + 2 genistein
2 dTDP + 7-O-(2-deoxy-beta-D-glucopyranosyl)genistein + 4'-O-(2-deoxy-beta-D-glucopyranosyl)genistein
-
-
-
?
2 UDP-D-glucose + corylifol A

2 UDP + corylifol A-4',7-di-O-beta-D-glucopyranoside
-
-
-
?
2 UDP-D-glucose + corylifol A
2 UDP + corylifol A-4',7-di-O-beta-D-glucopyranoside
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside + chrysin

2-nitrophenol + 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
-
-
-
r
2-nitrophenyl-beta-D-glucopyranoside + chrysin
2-nitrophenol + 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside + oroxylin A

2-nitrophenol + 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
-
-
-
r
2-nitrophenyl-beta-D-glucopyranoside + oroxylin A
2-nitrophenol + 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside + wogonin

2-nitrophenol + 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
-
-
-
r
2-nitrophenyl-beta-D-glucopyranoside + wogonin
2-nitrophenol + 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
-
-
-
?
ADP-glucose + 7,8-dihydroxyflavone

ADP + 7-O-beta-D-glucosyl-7,8-dihydroxyflavone
-
-
-
?
ADP-glucose + 7,8-dihydroxyflavone
ADP + 7-O-beta-D-glucosyl-7,8-dihydroxyflavone
-
-
-
?
CDP-glucose + 4',7-dihydroxyflavone

CDP + 7-O-beta-D-glucosyl-4',7-dihydroxyflavone
-
-
-
?
CDP-glucose + 4',7-dihydroxyflavone
CDP + 7-O-beta-D-glucosyl-4',7-dihydroxyflavone
-
-
-
?
TDP-glucose + curcumin

TDP + 7-O-beta-D-glucosyl-curcumin
-
-
-
?
TDP-glucose + curcumin
TDP + 7-O-beta-D-glucosyl-curcumin
-
-
-
?
UDP-D-glucose + corylifol A

UDP + corylifol A-4'-O-beta-D-glucopyranoside
-
-
-
?
UDP-D-glucose + corylifol A
UDP + corylifol A-4'-O-beta-D-glucopyranoside
-
-
-
?
UDP-glucose + 3-hydroxycoumarin

UDP + ?
high activity
-
-
?
UDP-glucose + 3-hydroxycoumarin
UDP + ?
higher activity
-
-
?
UDP-glucose + 4-coumaric acid

UDP + 1-O-[4-coumaroyl]-beta-D-glucopyranose
-
-
-
?
UDP-glucose + 4-coumaric acid
UDP + 1-O-[4-coumaroyl]-beta-D-glucopyranose
high activity
-
-
?
UDP-glucose + 7-hydroxyflavone

UDP + 7-O-beta-D-glucosyl-7-hydroxyflavone
-
-
-
?
UDP-glucose + 7-hydroxyflavone
UDP + 7-O-beta-D-glucosyl-7-hydroxyflavone
-
-
-
?
UDP-glucose + apigenin

UDP + ?
-
-
-
?
UDP-glucose + apigenin
UDP + ?
low activity
-
-
?
UDP-glucose + baicalein

UDP + ?
low activity
-
-
?
UDP-glucose + baicalein
UDP + ?
high activity
-
-
?
UDP-glucose + biochanin A

UDP + ?
-
-
-
?
UDP-glucose + biochanin A
UDP + ?
very low activity
-
-
?
UDP-glucose + caffeic acid

UDP + 1-O-caffeoyl-beta-D-glucopyranose
-
-
-
?
UDP-glucose + caffeic acid
UDP + 1-O-caffeoyl-beta-D-glucopyranose
low activity
-
-
?
UDP-glucose + chrysin

UDP + ?
-
-
-
?
UDP-glucose + chrysin
UDP + ?
low activity
-
-
?
UDP-glucose + ferulic acid

UDP + 1-O-feruolyl-beta-D-glucopyranose
-
-
-
?
UDP-glucose + ferulic acid
UDP + 1-O-feruolyl-beta-D-glucopyranose
higher activity
-
-
?
UDP-glucose + genistein

UDP + 4'-O-(beta-D-glucopyranosyl)genistein + 7-O-(beta-D-glucopyranosyl)genistein
-
-
-
?
UDP-glucose + genistein
UDP + 4'-O-(beta-D-glucopyranosyl)genistein + 7-O-(beta-D-glucopyranosyl)genistein
-
-
-
?
UDP-glucose + oroxylin A

UDP + ?
-
-
-
?
UDP-glucose + oroxylin A
UDP + ?
low activity
-
-
?
UDP-glucose + scutellarein

UDP + ?
low activity
-
-
?
UDP-glucose + scutellarein
UDP + ?
high activity
-
-
?
UDP-glucose + sinapic acid

UDP + 1-O-sinapoyl-beta-D-glucopyranose
-
-
-
?
UDP-glucose + sinapic acid
UDP + 1-O-sinapoyl-beta-D-glucopyranose
low activity
-
-
?
UDP-glucose + wogonin

UDP + ?
-
-
-
?
UDP-glucose + wogonin
UDP + ?
low activity
-
-
?
additional information

?
-
isoform UGT89C1 displays flexibility in terms of substrates and in using bacterial indigenous dTDP-beta-L-rhamnose as a sugar donor
-
-
-
additional information
?
-
UDP is preferred over other NDP-sugars. The conversion of 7-hydroxyflavone to its glucoside is 33.6, 19.4, 22.1 and 15% when TDP-, CDP-, ADP- and GDP-glucose are used
-
-
-
additional information
?
-
UDP is preferred over other NDP-sugars. The conversion of 7-hydroxyflavone to its glucoside is 33.6, 19.4, 22.1 and 15% when TDP-, CDP-, ADP- and GDP-glucose are used
-
-
-
additional information
?
-
the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75L25. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. Reversible glucosylation in the one-pot reaction coupled by UGT75L25 with oroxylin A, wogonin, and chrysin as acceptors and 2-nitrophenyl-beta-D-glucopyranoside as donor. Conversions of 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, and 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside are 58%, 20%, and 27%, respectively
-
-
-
additional information
?
-
the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75L25. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. Reversible glucosylation in the one-pot reaction coupled by UGT75L25 with oroxylin A, wogonin, and chrysin as acceptors and 2-nitrophenyl-beta-D-glucopyranoside as donor. Conversions of 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, and 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside are 58%, 20%, and 27%, respectively
-
-
-
additional information
?
-
-
the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75L25. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. Reversible glucosylation in the one-pot reaction coupled by UGT75L25 with oroxylin A, wogonin, and chrysin as acceptors and 2-nitrophenyl-beta-D-glucopyranoside as donor. Conversions of 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, and 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside are 58%, 20%, and 27%, respectively
-
-
-
additional information
?
-
the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75X1. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. No activity with chrysin, luteolin, quercetin, genistein, and vanillic acid with UDP-glucose as donor
-
-
-
additional information
?
-
the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75X1. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. No activity with chrysin, luteolin, quercetin, genistein, and vanillic acid with UDP-glucose as donor
-
-
-
additional information
?
-
-
the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75X1. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. No activity with chrysin, luteolin, quercetin, genistein, and vanillic acid with UDP-glucose as donor
-
-
-
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