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Information on EC 2.4.1.81 - flavone 7-O-beta-glucosyltransferase
for references in articles please use BRENDA:EC2.4.1.81
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.81 flavone 7-O-beta-glucosyltransferase
IUBMB Comments
A number of flavones, flavanones and flavonols can function as acceptors. Different from EC 2.4.1.91 (flavonol 3-O-glucosyltransferase).
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 2.4.1.81
- kaempferol
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rhamnosylated
- flavonols
- glucosides
- quercetin
-
isoflavonoids
- 3-o-glucoside
- rhamnosyltransferase
- udp-glucosyltransferase
- anthocyanins
- isoflavone
- aglycones
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glucosyltransferases
- synthesis
Reaction Schemes
showSynonyms
ugt89c1, ugt706d1, ugt75l25, ugt75x1, ugt73c20, ugt88e19, ugt92g4, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
glucosyltransferase, uridine diphosphoglucose-apigenin 7-O-
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glucosyltransferase, uridine diphosphoglucose-luteolin
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UDP-glucose-apigenin beta-glucosyltransferase
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UDP-glucosyltransferase
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UDPglucose-luteolin beta-D-glucosyltransferase
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uridine diphosphoglucose-apigenin 7-O-glucosyltransferase
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YjiC
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 5,7,3',4'-tetrahydroxyflavone = UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
UDP-glucose + 5,7,3',4'-tetrahydroxyflavone = UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
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UDP-glucose + 5,7,3',4'-tetrahydroxyflavone = UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
GTs are generally regarded as unidirectional catalysts that drive the formation of glycosidic bonds from NDP-sugar donors and aglycon acceptors according to a nucleophilic substitution mechanism (SN2). An SN2 attack by the aromatic hydroxyl or carboxylate oxygen to the anomeric carbon of UDP-glucose is performed in the aromatic O-glucoside or acyl-glucose ester forming reactions
UDP-glucose + 5,7,3',4'-tetrahydroxyflavone = UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
GTs are generally regarded as unidirectional catalysts that drive the formation of glycosidic bonds from NDP-sugar donors and aglycon acceptors according to a nucleophilic substitution mechanism (SN2). An SN2 attack by the aromatic hydroxyl or carboxylate oxygen to the anomeric carbon of UDP-glucose is performed in the aromatic O-glucoside or acyl-glucoseester forming reactions
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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PATHWAY SOURCE
PATHWAYS
MetaCyc
apigenin glycosides biosynthesis, luteolin glycosides biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:5,7,3',4'-tetrahydroxyflavone 7-O-beta-D-glucosyltransferase
A number of flavones, flavanones and flavonols can function as acceptors. Different from EC 2.4.1.91 (flavonol 3-O-glucosyltransferase).
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CAS REGISTRY NUMBER
COMMENTARY
37332-50-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 dTDP-2-deoxy alpha-D-glucose + 2 genistein
2 dTDP + 7-O-(2-deoxy-beta-D-glucopyranosyl)genistein + 4'-O-(2-deoxy-beta-D-glucopyranosyl)genistein
2-nitrophenyl-beta-D-glucopyranoside + chrysin
2-nitrophenol + 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
2-nitrophenyl-beta-D-glucopyranoside + oroxylin A
2-nitrophenol + 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
2-nitrophenyl-beta-D-glucopyranoside + wogonin
2-nitrophenol + 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
dTDP-beta-L-rhamnose + genistein
dTDP + 7-O-(alpha-L-rhamnopyranosyl)genistein
Substrates: -
Products: -
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?
TDP-glucose + luteolin
TDP + 7-O-beta-D-glucosylluteolin
-
Substrates: -
Products: -
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?
UDP-alpha-D-glucose + 3,7,4'-trihydroxyflavone
UDP + 7-O-beta-D-glucosyl-3,7,4'-trihydroxyflavone
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Substrates: 9% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + 5,7-dihydroxyflavanone
UDP + 7-O-beta-D-glucosyl-5,7-dihydroxyflavanone
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Substrates: 15% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + 7,4'-dihydroxyflavone
UDP + 7-O-beta-D-glucosyl-7,4'-dihydroxyflavone
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Substrates: 20% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + acacetin
UDP + 7-O-beta-D-glucosylacacetin
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Substrates: 24% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + apigenin
UDP + 7-O-beta-D-glucosylapigenin
-
Substrates: -
Products: -
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?
UDP-alpha-D-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
Substrates: 29.6% of the activity with eriodictyol
Products: -
Products: -
?
UDP-alpha-D-glucose + chrysoeriol
UDP + 7-O-beta-D-glucosylchrysoeriol
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Substrates: 63% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + datiscetin
UDP + 7-O-beta-D-glucosyldatiscetin
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Substrates: 9% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + fisetin
UDP + 7-O-beta-D-glucosylfisetin
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Substrates: 14% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + galangin
UDP + 7-O-beta-D-glucosylgalangin
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Substrates: 9% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + kaempferid
UDP + 7-O-beta-D-glucosylkaempferid
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Substrates: 16% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + kaempferol
UDP + 7-O-beta-D-glucosylkaempferol
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Substrates: 11% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
Substrates: 78.9% of the activity with eriodictyol
Products: -
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?
UDP-alpha-D-glucose + luteolin
UDP + 7-O-beta-D-glucosylluteolin
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Substrates: 210% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + naringenin
UDP + 7-O-beta-D-glucosylnaringenin
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Substrates: 45% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + quercetin
UDP + 7-O-beta-D-glucosylquercetin
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Substrates: 16% of the activity with apigenin
Products: -
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?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
Substrates: 64.1% of the activity with eriodictyol
Products: -
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?
UDP-galactose + daidzein
UDP + 7-O-beta-D-galactosyldaidzein
-
Substrates: -
Products: -
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?
UDP-galactose + genistein
UDP + 7-O-beta-D-galactosylgenistein
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Substrates: -
Products: -
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?
UDP-galactose + myricetin
UDP + 7-O-beta-D-galactosylmyricetin
-
Substrates: -
Products: -
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?
UDP-galactose + quercetin
UDP + 7-O-beta-D-galactosylquercetin
-
Substrates: -
Products: -
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?
UDP-glucose + 2-chlorocinnamic acid
UDP + 1-O-[2-chlorocinnamoyl]-beta-D-glucopyranose
Substrates: low activity
Products: -
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?
UDP-glucose + 2-coumaric acid
UDP + 1-O-[2-coumaroyl]-beta-D-glucopyranose
Substrates: high activity
Products: -
Products: -
?
UDP-glucose + 2-methylcinnamic acid
UDP + 1-O-[2-methylcinnamoyl]-beta-D-glucopyranose
Substrates: -
Products: -
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?
UDP-glucose + 3',4',5,7-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-3',4',5,7-tetrahydroxyflavone
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Substrates: involved in biosynthesis of flavone glucosides
Products: -
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?
UDP-glucose + 3-chlorocinnamic acid
UDP + 1-O-[3-chlorocinnamoyl]-beta-D-glucopyranose
Substrates: low activity
Products: -
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?
UDP-glucose + 5,7,3',4'-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
Substrates: -
Products: -
Products: -
?
UDP-glucose + apigenin
UDP + apigenin 7-O-glucoside
-
Substrates: -
Products: -
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?
UDP-glucose + daidzein
UDP + 7-O-beta-D-glucosyldaidzein
-
Substrates: -
Products: -
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?
UDP-glucose + eriodictyol
UDP + 7-O-beta-D-glucosyl-eriodictyol
Substrates: -
Products: -
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?
UDP-glucose + genistein
UDP + 4'-O-(beta-D-glucopyranosyl)genistein + 7-O-(beta-D-glucopyranosyl)genistein
UDP-glucose + genistein
UDP + 7-O-(beta-D-glucopyranosyl)genistein
Substrates: -
Products: -
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?
UDP-glucose + genistein
UDP + 7-O-beta-D-glucosylgenistein
-
Substrates: -
Products: -
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?
UDP-glucose + genistein
UDP + ?
Substrates: -
Products: -
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?
UDP-glucose + luteolin
UDP + 7-O-beta-D-glucosyl-luteolin
Substrates: 92.1% of the activity with eriodictyol
Products: -
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?
UDP-glucose + luteolin
UDP + ?
Substrates: -
Products: -
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?
UDP-glucose + myricetin
UDP + 7-O-beta-D-glucosylmyricetin
-
Substrates: -
Products: -
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?
UDP-glucose + naringenin
UDP + 7-O-beta-D-glucosyl-naringenin
Substrates: 71.6% of the activity with eriodictyol
Products: -
Products: -
?
UDP-glucose + naringenin
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + quercetin
UDP + ?
Substrates: -
Products: -
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?
UDP-glucose + vanillic acid
UDP + ?
Substrates: low activity
Products: -
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?
2 dTDP-2-deoxy alpha-D-glucose + 2 genistein
2 dTDP + 7-O-(2-deoxy-beta-D-glucopyranosyl)genistein + 4'-O-(2-deoxy-beta-D-glucopyranosyl)genistein
Substrates: -
Products: -
Products: -
?
2 dTDP-2-deoxy alpha-D-glucose + 2 genistein
2 dTDP + 7-O-(2-deoxy-beta-D-glucopyranosyl)genistein + 4'-O-(2-deoxy-beta-D-glucopyranosyl)genistein
Substrates: -
Products: -
Products: -
?
2 UDP-D-glucose + corylifol A
2 UDP + corylifol A-4',7-di-O-beta-D-glucopyranoside
Substrates: -
Products: -
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?
2 UDP-D-glucose + corylifol A
2 UDP + corylifol A-4',7-di-O-beta-D-glucopyranoside
Substrates: -
Products: -
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?
2-nitrophenyl-beta-D-glucopyranoside + chrysin
2-nitrophenol + 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
r
2-nitrophenyl-beta-D-glucopyranoside + chrysin
2-nitrophenol + 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
?
2-nitrophenyl-beta-D-glucopyranoside + oroxylin A
2-nitrophenol + 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
r
2-nitrophenyl-beta-D-glucopyranoside + oroxylin A
2-nitrophenol + 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
?
2-nitrophenyl-beta-D-glucopyranoside + wogonin
2-nitrophenol + 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
r
2-nitrophenyl-beta-D-glucopyranoside + wogonin
2-nitrophenol + 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
?
ADP-glucose + 7,8-dihydroxyflavone
ADP + 7-O-beta-D-glucosyl-7,8-dihydroxyflavone
Substrates: -
Products: -
Products: -
?
ADP-glucose + 7,8-dihydroxyflavone
ADP + 7-O-beta-D-glucosyl-7,8-dihydroxyflavone
Substrates: -
Products: -
Products: -
?
CDP-glucose + 4',7-dihydroxyflavone
CDP + 7-O-beta-D-glucosyl-4',7-dihydroxyflavone
Substrates: -
Products: -
Products: -
?
CDP-glucose + 4',7-dihydroxyflavone
CDP + 7-O-beta-D-glucosyl-4',7-dihydroxyflavone
Substrates: -
Products: -
Products: -
?
TDP-glucose + curcumin
TDP + 7-O-beta-D-glucosyl-curcumin
Substrates: -
Products: -
Products: -
?
TDP-glucose + curcumin
TDP + 7-O-beta-D-glucosyl-curcumin
Substrates: -
Products: -
Products: -
?
UDP-D-glucose + corylifol A
UDP + corylifol A-4'-O-beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
?
UDP-D-glucose + corylifol A
UDP + corylifol A-4'-O-beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + 3-hydroxycoumarin
UDP + ?
Substrates: high activity
Products: -
Products: -
?
UDP-glucose + 3-hydroxycoumarin
UDP + ?
Substrates: higher activity
Products: -
Products: -
?
UDP-glucose + 4-coumaric acid
UDP + 1-O-[4-coumaroyl]-beta-D-glucopyranose
Substrates: -
Products: -
Products: -
?
UDP-glucose + 4-coumaric acid
UDP + 1-O-[4-coumaroyl]-beta-D-glucopyranose
Substrates: high activity
Products: -
Products: -
?
UDP-glucose + 7-hydroxyflavone
UDP + 7-O-beta-D-glucosyl-7-hydroxyflavone
Substrates: -
Products: -
Products: -
?
UDP-glucose + 7-hydroxyflavone
UDP + 7-O-beta-D-glucosyl-7-hydroxyflavone
Substrates: -
Products: -
Products: -
?
UDP-glucose + apigenin
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + apigenin
UDP + ?
Substrates: low activity
Products: -
Products: -
?
UDP-glucose + baicalein
UDP + ?
Substrates: low activity
Products: -
Products: -
?
UDP-glucose + baicalein
UDP + ?
Substrates: high activity
Products: -
Products: -
?
UDP-glucose + biochanin A
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + biochanin A
UDP + ?
Substrates: very low activity
Products: -
Products: -
?
UDP-glucose + caffeic acid
UDP + 1-O-caffeoyl-beta-D-glucopyranose
Substrates: -
Products: -
Products: -
?
UDP-glucose + caffeic acid
UDP + 1-O-caffeoyl-beta-D-glucopyranose
Substrates: low activity
Products: -
Products: -
?
UDP-glucose + chrysin
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + chrysin
UDP + ?
Substrates: low activity
Products: -
Products: -
?
UDP-glucose + ferulic acid
UDP + 1-O-feruolyl-beta-D-glucopyranose
Substrates: -
Products: -
Products: -
?
UDP-glucose + ferulic acid
UDP + 1-O-feruolyl-beta-D-glucopyranose
Substrates: higher activity
Products: -
Products: -
?
UDP-glucose + genistein
UDP + 4'-O-(beta-D-glucopyranosyl)genistein + 7-O-(beta-D-glucopyranosyl)genistein
Substrates: -
Products: -
Products: -
?
UDP-glucose + genistein
UDP + 4'-O-(beta-D-glucopyranosyl)genistein + 7-O-(beta-D-glucopyranosyl)genistein
Substrates: -
Products: -
Products: -
?
UDP-glucose + oroxylin A
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + oroxylin A
UDP + ?
Substrates: low activity
Products: -
Products: -
?
UDP-glucose + scutellarein
UDP + ?
Substrates: low activity
Products: -
Products: -
?
UDP-glucose + scutellarein
UDP + ?
Substrates: high activity
Products: -
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?
UDP-glucose + sinapic acid
UDP + 1-O-sinapoyl-beta-D-glucopyranose
Substrates: -
Products: -
Products: -
?
UDP-glucose + sinapic acid
UDP + 1-O-sinapoyl-beta-D-glucopyranose
Substrates: low activity
Products: -
Products: -
?
UDP-glucose + wogonin
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + wogonin
UDP + ?
Substrates: low activity
Products: -
Products: -
?
additional information
?
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Substrates: isoform UGT89C1 displays flexibility in terms of substrates and in using bacterial indigenous dTDP-beta-L-rhamnose as a sugar donor
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additional information
?
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Substrates: UDP is preferred over other NDP-sugars. The conversion of 7-hydroxyflavone to its glucoside is 33.6, 19.4, 22.1 and 15% when TDP-, CDP-, ADP- and GDP-glucose are used
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additional information
?
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Substrates: UDP is preferred over other NDP-sugars. The conversion of 7-hydroxyflavone to its glucoside is 33.6, 19.4, 22.1 and 15% when TDP-, CDP-, ADP- and GDP-glucose are used
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additional information
?
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Substrates: the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75L25. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. Reversible glucosylation in the one-pot reaction coupled by UGT75L25 with oroxylin A, wogonin, and chrysin as acceptors and 2-nitrophenyl-beta-D-glucopyranoside as donor. Conversions of 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, and 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside are 58%, 20%, and 27%, respectively
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Products: -
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additional information
?
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Substrates: the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75L25. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. Reversible glucosylation in the one-pot reaction coupled by UGT75L25 with oroxylin A, wogonin, and chrysin as acceptors and 2-nitrophenyl-beta-D-glucopyranoside as donor. Conversions of 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, and 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside are 58%, 20%, and 27%, respectively
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Products: -
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additional information
?
-
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Substrates: the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75L25. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. Reversible glucosylation in the one-pot reaction coupled by UGT75L25 with oroxylin A, wogonin, and chrysin as acceptors and 2-nitrophenyl-beta-D-glucopyranoside as donor. Conversions of 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside, and 5-hydroxy-4-oxo-2-phenyl-4H-1-benzopyran-7-yl beta-D-glucopyranoside are 58%, 20%, and 27%, respectively
Products: -
Products: -
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additional information
?
-
Substrates: the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75X1. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. No activity with chrysin, luteolin, quercetin, genistein, and vanillic acid with UDP-glucose as donor
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additional information
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Substrates: the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75X1. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. No activity with chrysin, luteolin, quercetin, genistein, and vanillic acid with UDP-glucose as donor
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additional information
?
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Substrates: the enzyme exhibits broad substrate specificity toward 21 structurally diverse types of phenolic acids, including (hydroxy)cinnamates, vanillic acid, 3-hydroxycoumarin, and 7-hydroxyflavonoids, overview. The optimized model reactions are performed with 2-nitrophenyl-beta-D-glucopyranoside as a sugar donor and diverse substrates as acceptors under the catalysis of UGT75X1. With a relatively broad substrate promiscuity, diverse 1-O-acyl-glucose-esters and glucosides are produced by the EbUGTs. No activity with chrysin, luteolin, quercetin, genistein, and vanillic acid with UDP-glucose as donor
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 3',4',5,7-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-3',4',5,7-tetrahydroxyflavone
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Substrates: involved in biosynthesis of flavone glucosides
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UDP-glucose + 5,7,3',4'-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
Substrates: -
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Tris-HCl
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in 1 M Tris-HCl buffer the reaction rate is 70% of that observed in 0.1 M buffer
additional information
no inhibition by EDTA; no inhibition by EDTA
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additional information
no inhibition by EDTA; no inhibition by EDTA
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.88
corylifol A
pH 9.6, temperature not specified in the publication
0.0699
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.088
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.1288
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.2208
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.254
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.329
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
2.07
TDP-glucose
wild-type, pH 8.0, temperature not specified in the publication
2.19
TDP-glucose
mutant R282W, pH 8.0, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0097
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.0125
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.0147
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.0332
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.0338
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.0505
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.067
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.097
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.103
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.139
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.199
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
0.377
4-coumaric acid
pH 6.0, 40°C, recombinant enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 9
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pH 6.0: about 35% of maximal activity, pH 9.0: about 50% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
