the expression of UFGT3a is highly consistent with anthocyanin accumulation. Gene silencing in fruit results in delayed red colorations, lower anthocyanin contents and lower expressions of UFGT3a. Transient overexpression of UFGT3a in both Actinidia arguta and green apple fruit results in higher anthocyanin contents and deeper red coloration
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native enzyme 526fold by ammonium sulfate fractionation and ultracentrifugation, the enzyme is then separated from the UDP-xylose:cyanidin 3-galactoside xylosyltransferase, CGXT, by anion exchange and affinity chromatography, followed by gel filtation