We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.4.1.287 - rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase for references in articles please use BRENDA:EC2.4.1.287Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 2 UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
overall reaction
-
-
-
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol = UDP + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
(1a)
-
-
-
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol = UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
(1b)
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactofuranose:alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/4-beta-galactofuranosyltransferase (configuration-inverting)
Isolated from the bacteria Mycobacterium tuberculosis and M. smegmatis, the enzyme has dual beta-(1->4) and beta-(1->5) transferase action. Involved in the formation of the cell wall in mycobacteria.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
arabinogalactan galactofuranosyl transferase 1
-
-
-
-
galactofuranosyl transferase 1
-
-
galactosyl transferase
-
-
galactosyl transferase
-
-
-
GalTr
-
-
GlfT1
-
-
-
-
Rv3782
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
-
the enzyme is involved in the initial steps of galactan formation gand initiates galactan synthesis on the C50-P-P-GlcNAc-Rha acceptor
physiological function
-
the enzyme is involved in biosynthesis of the mycobacterial cell wall
physiological function
-
the enzyme is involved in biosynthesis of the mycobacterial cell wall
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
additional information
?
-
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
synthetic substrate surrogate. Substitution of the farnesol group to isoprenol reduces activity by about 75%
-
-
-
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-(2Z,6Z)-farnesol
synthetic substrate surrogate. Substitution of the farnesol group to isoprenol reduces activity by about 75%
-
-
-
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
-
-
-
-
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
-
-
-
-
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
-
-
-
?
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
-
-
-
?
UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
-
-
-
?
additional information
?
-
-
the enzyme harbors dual beta-(1->4) and beta-(1->5) galactofuranose transferase activities. The enzyme cannot catalyze subsequent galactan chain extension. Gal-Gal-Gal-Rha-GlcNAc-P-P-decaprenol is not a substrate
-
-
-
additional information
?
-
-
the bifunctional enzyme is responsible for the initial synthesis of C50-P-P-GlcNAc-Rha-Galf, followed by immediate conversion to C50-P-P-GlcNAc-Rha-Galf-Galf
-
-
-
additional information
?
-
enzyme requires a substrate bearing the disaccharide L-Rha-alpha-(1,3)-D-GlcNAc. Phosphonophosphate analogues serve as effective substrates, being processed to lipid-linked oligosaccharides
-
-
-
additional information
?
-
enzyme requires a substrate bearing the disaccharide L-Rha-alpha-(1,3)-D-GlcNAc. Phosphonophosphate analogues serve as effective substrates, being processed to lipid-linked oligosaccharides
-
-
-
additional information
?
-
-
the bifunctional enzyme is responsible for the initial synthesis of C50-P-P-GlcNAc-Rha-Galf, followed by immediate conversion to C50-P-P-GlcNAc-Rha-Galf-Galf
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
-
brenda
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
33863
-
x * 33863, calculated from amino acid sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 33863, calculated from amino acid sequence
?
-
x * 33863, calculated from amino acid sequence
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Talon resin column chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Mycobacterium smegmatis mc2155 cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GLFT1_MYCS2
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
302
34041
Swiss-Prot
GLFT1_MYCTO
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
304
33864
Swiss-Prot
GLFT1_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
304
33864
Swiss-Prot
A0A0M2H5X7_9MICO
325
37077
TrEMBL
A0A0G3HG87_9CORY
299
33616
TrEMBL
A0A0M9AHN9_THEAQ
304
35484
TrEMBL
A0A0M5IFN4_9CORY
310
35009
TrEMBL
A0A0J5S917_9LACT
299
34391
TrEMBL
A0A0G3GY57_9CORY
310
35194
TrEMBL
A0A2C8D541_CORDP
305
34262
TrEMBL
A0A178XA85_9PSEU
303
33782
TrEMBL
A0A0C3ACS6_RHOER
298
33488
TrEMBL
A0A231HCJ6_9NOCA
307
34333
TrEMBL
A0A0F6R0A5_9CORY
300
33754
TrEMBL
A0A0B6EZR7_9CORY
304
34345
TrEMBL
A0A1Q2HTD3_9CORY
288
32884
TrEMBL
A0A1G4WLU0_CORJE
302
33623
TrEMBL
A0A0J6VYE7_9MYCO
298
33821
TrEMBL
A0A0J6VKL8_9MYCO
299
33775
TrEMBL
A0A1V2RDD9_9ACTN
292
32361
TrEMBL
A0A1M4MRF9_METWO
324
37379
TrEMBL
A0A1Y5P6W2_9MYCO
297
33173
TrEMBL
A0A223ZDZ1_9EURY
158
17715
TrEMBL
A0A0F0KCH5_9MICO
318
36599
TrEMBL
A0A0F2C4M7_9MICO
320
36528
TrEMBL
A0A2S8BSK2_9MYCO
299
33589
TrEMBL
A0A085ETY8_9RHIZ
350
38412
TrEMBL
A0A2V2BNI6_9EURY
348
40737
TrEMBL
A0A0Q0UDH6_9CORY
302
34199
TrEMBL
A0A1Y2N3X0_PSEAH
301
33272
TrEMBL
A0A075ULC0_9PSEU
302
33870
TrEMBL
A0A288Q8P9_9LACT
298
33719
TrEMBL
A0A1Z4EPY2_9MYCO
305
34257
TrEMBL
A0A0G3H735_9CORY
308
34683
TrEMBL
A0A161GLP6_RHOFA
304
34209
TrEMBL
A0A0H5D2B7_9RHOB
364
39703
TrEMBL
A0A0B8NAB6_9NOCA
307
34488
TrEMBL
A0A0G3GLP8_9CORY
299
33297
TrEMBL
A0A1X7AS09_9GAMM
323
37868
TrEMBL
A0A0F0KU73_9MICO
292
32201
TrEMBL
A0A0C1L6V1_9PSEU
299
33661
TrEMBL
A0A098BGT6_9NOCA
299
33596
TrEMBL
A0A143QCN9_9NOCA
295
33196
TrEMBL
A0A0K2YRX1_9NOCA
294
33007
TrEMBL
A0A1X7A2Q5_9RHOB
325
35612
TrEMBL
A0A0B6TIN2_9CORY
306
34484
TrEMBL
A0A1D8BQC2_9PSEU
317
35254
TrEMBL
A0A1L7F024_9PSEU
315
35073
TrEMBL
A0A0J6VVZ4_9MYCO
299
33832
TrEMBL
A0A222TDA8_GORRU
305
34117
TrEMBL
A0A1B1JXR9_RHOOP
303
34103
TrEMBL
A0A0D1M9H2_9LACT
299
34319
TrEMBL
A0A2N7RR83_9BURK
285
31860
TrEMBL
A0A0X2NMA9_9CORY
318
35368
TrEMBL
A0A085FQL8_9BURK
326
35093
TrEMBL
A0A0K2X0K9_CORST
304
34142
TrEMBL
A0A288QWK7_9LACT
307
34567
TrEMBL
A0A221VW93_9PSEU
315
34975
TrEMBL
A0A143QIM0_9NOCA
304
34167
TrEMBL
A0A1V5ZVI1_9BACT
306
35579
TrEMBL
A0A2C9SRE6_9MYCO
294
33431
TrEMBL
A0A1V4VIY1_9FIRM
241
27811
TrEMBL
A0A238LIM1_9RHOB
328
36271
TrEMBL
A0A0F0LTL2_9MICO
328
36962
TrEMBL
A0A0Q0ZB16_9CORY
302
34039
TrEMBL
A0A223YWH2_9EURY
328
37533
TrEMBL
A0A1D7ZVJ1_LACFE
296
33978
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mikusova, K.; Belanova, M.; Kordulakova, J.; Honda, K.; McNeil, M.; Mahapatra, S.; Crick, D.; Brennan, P.
Identification of a novel galactosyl transferase involved in biosynthesis of the mycobacterial cell wall
J. Bacteriol.
188
6592-6598
2006
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Belanova, M.; Dianiskova, P.; Brennan, P.J.; Completo, G.C.; Rose, N.L.; Lowary, T.L.; Mikusova, K.
Galactosyl transferases in mycobacterial cell wall synthesis
J. Bacteriol.
190
1141-1145
2008
Mycobacterium smegmatis
brenda
Martinez Farias, M.A.; Kincaid, V.A.; Annamalai, V.R.; Kiessling, L.L.
Isoprenoid phosphonophosphates as glycosyltransferase acceptor substrates
J. Am. Chem. Soc.
136
8492-8495
2014
Mycobacterium tuberculosis (P9WMX3), Mycobacterium tuberculosis ATCC 25618 (P9WMX3)
brenda
html completed