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Information on EC 2.4.1.215 - cis-zeatin O-beta-D-glucosyltransferase and Organism(s) Zea mays and UniProt Accession Q93XP7

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.215 cis-zeatin O-beta-D-glucosyltransferase
IUBMB Comments
The enzyme from maize can use cis-zeatin and UDP-glucose as substrates, but not cis-ribosylzeatin, trans-zeatin or trans-ribosylzeatin. Unlike EC 2.4.1.203, trans-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose cannot act as a donor.
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This record set is specific for:
Zea mays
UNIPROT: Q93XP7
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Word Map
The taxonomic range for the selected organisms is: Zea mays
The enzyme appears in selected viruses and cellular organisms
Synonyms
ciszog1, osczog1, zeatin o-glucosyltransferase, czogt2, cis-zeatin o-glucosyltransferase, czogt1, czogt3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cis-zeatin O-beta-D-glucosyltransferase
-
cisZOG1
-
zeatin glycosyltransferase
GT
zeatin O-glucosyltransferase
-
cisZOG1
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of hexosyl group
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:cis-zeatin O-beta-D-glucosyltransferase
The enzyme from maize can use cis-zeatin and UDP-glucose as substrates, but not cis-ribosylzeatin, trans-zeatin or trans-ribosylzeatin. Unlike EC 2.4.1.203, trans-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose cannot act as a donor.
CAS REGISTRY NUMBER
COMMENTARY hide
123644-76-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + cis-zeatin
UDP + O-beta-D-glucosyl-cis-zeatin
show the reaction diagram
UDP-glucose + cis-zeatin
UDP + O-beta-D-glucosyl-cis-zeatin
show the reaction diagram
-
-
-
-
?
UDP-glucose + o-hydroxythidiazuron
UDP + ?
show the reaction diagram
-
128% of the activity with trans-zeatin
-
-
?
UDP-glucose + o-topolin
UDP + O-beta-D-glucosyl-o-topolin
show the reaction diagram
-
490% of the activity with cis-zeatin
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
benzyladenine
-
kinetin
-
N6-(DELTA2-isopentenyl)adenine
-
thidiazuron
-
trans-zeatin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.046
cis-zeatin
cisZOG1
0.11
UDP-glucose
cisZOG1
0.05
cis-zeatin
-
-
0.18
o-topolin
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
cis-zeatin
-
-
0.03
o-topolin
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
theoretical value
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
lower level of mRNA
Manually annotated by BRENDA team
high expression of cisZOG1
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CZOG1_MAIZE
467
0
51129
Swiss-Prot
Secretory Pathway (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51100
estimated from gene sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
transgenic Zea mays plants are obtained using an Agrobacterium mediated transformation procedure, Ubi : ZOG1 maize line overexpressing the ZOG1 gene of Phaseolus lunatus display increased levels of zeatin-O-glucoside, zeatin O-glucosylation affects root formation, leaf development, chlorophyll content, senescence, male flower differentiation and the formation of tasselseed
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Martin, R.C.; Mok, M.C.; Habben, J.E.; Mok, D.W.S.
A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-zeatin
Proc. Natl. Acad. Sci. USA
98
5922-5926
2001
Zea mays (Q93XP7), Zea mays
Manually annotated by BRENDA team
Veach, Y.K.; Martin, R.C.; Mok, D.W.; Malbeck, J.; Vankova, R.; Mok, M.C.
O-Glucosylation of cis-zeatin in maize. Characterization of genes, enzymes, and endogenous cytokinins
Plant Physiol.
131
1374-1380
2003
Zea mays (Q93XP7), Zea mays
Manually annotated by BRENDA team
Mok, M.C.; Martin, R.C.; Dobrev, P.I.; Vankova, R.; Ho, P.S.; Yonekura-Sakakibara, K.; Sakakibara, H.; Mok, D.W.
Topolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition
Plant Physiol.
137
1057-1066
2005
Zea mays
Manually annotated by BRENDA team
Rodo, A.P.; Brugiere, N.; Vankova, R.; Malbeck, J.; Olson, J.M.; Haines, S.C.; Martin, R.C.; Habben, J.E.; Mok, D.W.; Mok, M.C.
Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation
J. Exp. Bot.
59
2673-2686
2008
Zea mays (Q93XP7), Zea mays
Manually annotated by BRENDA team
Meek, L.; Martin, R.C.; Shan, X.; Karplus, P.A.; Mok, D.W.; Mok, M.C.
Isolation of Legume Glycosyltransferases and Active Site Mapping of the Phaseolus lunatus Zeatin O-glucosyltransferase ZOG1
J. Plant Growth Regul.
27
192-201
2008
Zea mays (Q93XP7)
-
Manually annotated by BRENDA team