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Information on EC 2.4.1.126 - hydroxycinnamate 4-beta-glucosyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession O23402

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.126 hydroxycinnamate 4-beta-glucosyltransferase
IUBMB Comments
Acts on 4-coumarate, ferulate, caffeate and sinapate, forming a mixture of 4-glucosides and glucose esters (cf. EC 2.4.1.120 sinapate 1-glucosyltransferase).
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O23402
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Word Map
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  • 2.4.1.126
  • calmodulin-binding
  • heat-stable
  • postmeiotic
  • calmodulin
  • footprint
  • testis-specific
  • calmodulin-dependent
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
glucosyltransferase, uridine diphosphoglucose-hydroxycinnamate, hydroxycinnamoyl glucosyltransferase, UDP-glucose-hydroxycinnamate glucosyltransferase, UDPglucose:trans-4-hydroxycinnamate 4-O-beta-D-glucosyltransferase, uridine diphosphoglucose-hydroxycinnamate glucosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucosyltransferase, uridine diphosphoglucose-hydroxycinnamate
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hydroxycinnamoyl glucosyltransferase
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UDP-glucose-hydroxycinnamate glucosyltransferase
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UDPglucose:trans-4-hydroxycinnamate 4-O-beta-D-glucosyltransferase
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uridine diphosphoglucose-hydroxycinnamate glucosyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:trans-4-hydroxycinnamate 4-O-beta-D-glucosyltransferase
Acts on 4-coumarate, ferulate, caffeate and sinapate, forming a mixture of 4-glucosides and glucose esters (cf. EC 2.4.1.120 sinapate 1-glucosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
77848-85-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 4-hydroxycinnamate
UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
show the reaction diagram
-
-
-
?
UDP-alpha-D-glucose + sinapate
UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
show the reaction diagram
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 4-hydroxycinnamate
UDP + 4-O-(beta-D-glucopyranosyl)-4-hydroxycinnamate
show the reaction diagram
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low activity in mature leaves
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
phenylpropanoid metabolism
metabolism
phenylpropanoid metabolism
physiological function
specific role for UGT84A3 in the accumulation of cell wall-bound 4-coumarate
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
U84A4_ARATH
475
0
53496
Swiss-Prot
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred; binary plasmid constructs for transformation of Arabidopsis are introduced into Agrobacterium tumefaciens by electroporation, plants are transformed by the floral dip method, for selection of transgenic plants T1 seeds are surface-sterilized in 70% ethanol for 2 min followed by a mixture of Tween 20 and sodium hypochloride for 10 min, seeds are rinsed thoroughly with sterile water and after swelling over night at 4°C plated on modified MS medium supplemented with carbenicillin and kanamycin, after scoring the development of antibiotic damage symptoms for 14 days post treatment, kanamycin resistant plants are transferred
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, overexpression plants did not display changes in UGT specificity of leave extracts, the overexpressing line UGT84A4 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, compared to the wild type, crude protein extracts from leaves of UGT84A1 overexpression plants displayed increased activity toward 4-coumarate and ferulate, the preferred in vitro substrates, the overexpressing line UGT84A1 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A1, overexpression of UGT84A1 results in a significant increase of cell wall-bound sinapate
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, leaves of UGT84A3 overexpression displayed a significantly higher capacity to synthesize feruloylglucose, the overexpressing line UGT84A3 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A3, analysis of liberated from cell walls reveals for UGT84A3 a significant increase in cell wall-associated 4-coumarate, ferulate and sinapate
exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and leads to a transient increase in sinapoylglucose and sinapoylmalate concentrations, UGT84A2 overexpression caused increasing activity toward sinapate, the overexpressing line UGT84A2 exhibits increased levels of sinapoylglucose in seeds and seedlings, the seed sinapine content is slightly increased in UGT84A2, mature leaves of UGT84A2 show a slight increase in the sinapoylmalate content, overexpression of UGT84A2 results in a significant increase of cell wall-bound sinapate
given the impact of UGT84A3 overexpression on the amount of cell wall-associated hydroxycinnamates, analysis of the cell wall fraction of the insertion knock-out mutant UGT84A3 shows a significant decrease of the 4-coumarate content in cell walls of null mutant whereas the levels of ferulate and sinapate are not changed compared to the wild type
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Meissner, D.; Albert, A.; Boettcher, C.; Strack, D.; Milkowski, C.
The role of UDP-glucose:hydroxycinnamate glucosyltransferases in phenylpropanoid metabolism and the response to UV-B radiation in Arabidopsis thaliana
Planta
228
663-674
2008
Arabidopsis thaliana (O23402)
Manually annotated by BRENDA team