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Information on EC 2.3.1.B43 - protein-lysine desuccinylase (NAD+) and Organism(s) Danio rerio and UniProt Accession Q6DHI5

for references in articles please use BRENDA:EC2.3.1.B43
preliminary BRENDA-supplied EC number
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Danio rerio
UNIPROT: Q6DHI5
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The taxonomic range for the selected organisms is: Danio rerio
The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria
Synonyms
sirt5, sirtuin 5, nad+-dependent protein deacetylase, nad+ dependent deacetylase, nicotinamide adenine dinucleotide-dependent protein deacetylase, sirtuin deacylase, nad+-dependent protein deacylase, histone desuccinylase, nad+-dependent sirtuin deacetylase, sirt5iso1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sirtuin 5 deacylase
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sirtuin-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
NAD+ + [protein]-N6-succinyl-L-lysine
nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NAD+ + [protein]-N6-succinyl-L-lysine
nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-benzylglycylglycyl-L-valyl-L-leucyl-N6-[(3R)-3-(benzylsulfanyl)-3-carboxypropanoyl]-L-lysyl-L-alpha-glutamyl-L-tyrosylglycyl-L-valinamide
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N-benzylglycylglycyl-L-valyl-L-leucyl-N6-[(3R)-3-carboxy-3-(phenylsulfanyl)propanoyl]-L-lysyl-L-alpha-glutamyl-L-tyrosylglycyl-L-valinamide
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N-benzylglycylglycyl-L-valyl-L-leucyl-N6-[(3S)-3-(benzylsulfanyl)-3-carboxypropanoyl]-L-lysyl-L-alpha-glutamyl-L-tyrosylglycyl-L-valinamide
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N-benzylglycylglycyl-L-valyl-L-leucyl-N6-[(3S)-3-carboxy-3-(phenylsulfanyl)propanoyl]-L-lysyl-L-alpha-glutamyl-L-tyrosylglycyl-L-valinamide
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additional information
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
sirtuins are a family of NAD+-dependent silent information regulator 2 (Sir2) enzymes that catalyze the removal of acyl groups from epsilon-N-amino groups of lysine residues in the proteome
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SIR5_DANRE
305
0
33929
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant detagged enzyme in complex with peptide inhibitors 2 and 15, sitting drop vapor diffusion method, mixing of 10 mg/ml protein with 1 mM inhibitor, crystallization in 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, and 20% PEG 3350, 20°C, 2-4 days, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement with search model PDB 4UTV and modeling
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, tag cleavage by TEV protease, reverse cobalt affinity chromatography, and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene SIRT5, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rajabi, N.; Auth, M.; Troelsen, K.R.; Pannek, M.; Bhatt, D.P.; Fontenas, M.; Hirschey, M.D.; Steegborn, C.; Madsen, A.S.; Olsen, C.A.
Mechanism-based inhibitors of the human sirtuin 5 deacylase structure-activity relationship, biostructural, and kinetic insight
Angew. Chem. Int. Ed. Engl.
56
14836-14841
2017
Danio rerio (Q6DHI5), Danio rerio, Homo sapiens (Q9NXA8), Homo sapiens
Manually annotated by BRENDA team
Kalbas, D.; Liebscher, S.; Nowak, T.; Meleshin, M.; Pannek, M.; Popp, C.; Alhalabi, Z.; Bordusa, F.; Sippl, W.; Steegborn, C.; Schutkowski, M.
Potent and selective inhibitors of human sirtuin 5
J. Med. Chem.
61
2460-2471
2018
Danio rerio (Q6DHI5), Homo sapiens (Q9NXA8), Homo sapiens
Manually annotated by BRENDA team