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Information on EC 2.3.1.20 - diacylglycerol O-acyltransferase and Organism(s) Bos taurus and UniProt Accession Q8MK44

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EC Tree
IUBMB Comments
Palmitoyl-CoA and other long-chain acyl-CoAs can act as donors.
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This record set is specific for:
Bos taurus
UNIPROT: Q8MK44
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The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
dgat1, dgat2, dgat, diacylglycerol acyltransferase, diacylglycerol acyltransferase 1, diacylglycerol o-acyltransferase, dgat-1, monoacylglycerol acyltransferase, acyl-coa:diacylglycerol acyltransferase, diacylglycerol acyltransferase 2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diacylglycerol acyltransferase 1
-
1,2-diacylglycerol acyltransferase
-
-
-
-
acyl-CoA:1,2-dioleoyl-sn-glycerol acyltransferase
-
-
-
-
acyltransferase, diacylglycerol
-
-
-
-
DGAT
-
-
-
-
DGAT1
-
-
-
-
DGAT1A
-
-
-
-
DGAT2
-
-
-
-
DGAT2A
-
-
-
-
diacylglycerol acyltransferase
-
-
-
-
diglyceride acyltransferase
-
-
-
-
diglyceride O-acyltransferase
-
-
-
-
palmitoyl-CoA-sn-1,2-diacylglycerol acyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
Palmitoyl-CoA and other long-chain acyl-CoAs can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-98-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
show the reaction diagram
acyl-CoA + 1,2-dipalmitoylglycerol
CoA + 3-acyl-1,2-dipalmitoylglycerol
show the reaction diagram
-
membrane bound 1,2-dipalmitoylglycerol
-
-
?
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
show the reaction diagram
-
-
-
-
?
hexanoyl-CoA + 1,2-dipalmitoyl glycerol
coenzyme A + 1,2-dipalmitoyl-3-hexanoyl glycerol
show the reaction diagram
-
-
-
-
r
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + dipalmitoylglycerol
CoA + tripalmitoylglycerol
show the reaction diagram
-
membrane-bound dipalmitoylglycerol
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
binds palmitoyl-CoA and decreases activity towards palmitoyl-CoA, but not butyryl-CoA
-
butyryl-CoA
-
-
N-ethylmaleimide
-
95% loss of activity after preincubation with 40 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016 - 0.025
butyryl-CoA
-
-
0.0057 - 0.0068
hexanoyl-CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
total enzyme activity derived from dehydrated muscle tissue is similar to, or better than, the total activity of enzyme from fresh tissue
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
assay at various temperatures from 24°C to 40°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DGAT1_BOVIN
489
8
55602
Swiss-Prot
other Location (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K232A
naturally occuring mutation, association with milk fatty acid composition
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing causes loss of 40% of the activity
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
dehydration is an effective method of storage of samples destined for estimation of the microsomal enzyme activity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Marshall, M.O.; Knudsen, J.
Specificity of diacylglycerol acyltransferase from bovine mammary gland, liver and adipose tissue towards acyl-CoA esters
Eur. J. Biochem.
94
93-98
1979
Bos taurus, Capra hircus, Oryctolagus cuniculus, Rattus norvegicus
Manually annotated by BRENDA team
Marshall, M.O.; Knudsen, J.
Factors influencing the in vitro activity of diacylglycerol acyltransferase from bovine mammary gland and liver towards butyryl-CoA and palmitoyl-CoA
Biochim. Biophys. Acta
617
393-397
1980
Bos taurus
Manually annotated by BRENDA team
Lozeman, F.J.; Middleton, C.K.; Deng, J.; Kazala, E.C.; Verhaege, C.; Mir, P.S.; Laroche, A.; Bailey, D.R.C.; Weselake, R.J.
Characterization of microsomal diacylglycerol acyltransferase activity from bovine adipose and muscle tissue
Comp. Biochem. Physiol. B
130B
105-115
2001
Bos taurus
-
Manually annotated by BRENDA team
Huff, P.W.; Lozeman, F.J.; Kazala, E.C.; Prozniak, C.; Wegner, J.; Deng, J.; Laroche, A.; Mir, P.S.; Aalhus, J.; Weselake, R.J.
Stability of diacylglycerol acyltransferase in dehydrated bovine muscle tissue
Anal. Biochem.
318
254-259
2003
Bos taurus
Manually annotated by BRENDA team
Talhari, D.T.; Moraes, M.L.; Castilho, P.V.; Oliveira, O.N.; Beltramini, L.M.; Araujo, A.P.
Interaction of a C-terminal peptide of Bos taurus diacylglycerol acyltransferase 1 with model membranes
Biochim. Biophys. Acta
1788
2320-2325
2009
Bos taurus (Q8MK44), Bos taurus
Manually annotated by BRENDA team
Conte, G.; Mele, M.; Chessa, S.; Castiglioni, B.; Serra, A.; Pagnacco, G.; Secchiari, P.
Diacylglycerol acyltransferase 1, stearoyl-CoA desaturase 1, and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle
J. Dairy Sci.
93
753-763
2010
Bos taurus (Q8MK44), Bos taurus
Manually annotated by BRENDA team