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Information on EC 1.7.1.16 - nitrobenzene nitroreductase and Organism(s) Enterobacter cloacae and UniProt Accession Q01234

for references in articles please use BRENDA:EC1.7.1.16
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EC Tree
IUBMB Comments
Contains FMN. The enzyme, characterized from Pseudomonas species, catalyses two successive reductions of nitrobenzene, via a nitrosobenzene intermediate. It is also active on 1-chloro-4-nitrobenzene.
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This record set is specific for:
Enterobacter cloacae
UNIPROT: Q01234
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Word Map
The taxonomic range for the selected organisms is: Enterobacter cloacae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
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Synonyms
nitrobenzene nitroreductase, oxygen-insensitive nad(p)h nitroreductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
N-phenylhydroxylamine:NADP+ oxidoreductase
Contains FMN. The enzyme, characterized from Pseudomonas species, catalyses two successive reductions of nitrobenzene, via a nitrosobenzene intermediate. It is also active on 1-chloro-4-nitrobenzene.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NFSB_ENTCL
217
0
23950
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
molecular dynamics simulation of free enzyme in oxidized form, fully reduced enzyme with benzoate inhibitor and fully reduced enzyme with nitrobenzene. The complexed nitroreductase becomes more flexible overall upon complexation, particularly helix H6, in the vicinity of the binding site. Five regions which are highly conserved within the flavin mononucleotide (FMN) binding site are identified
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Isayev, O.; Crespo-Hernandez, C.E.; Gorb, L.; Hill, F.C.; Leszczynski, J.
In silico structure-function analysis of E. cloacae nitroreductase
Proteins
80
2728-2741
2012
Enterobacter cloacae (Q01234)
Manually annotated by BRENDA team