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Information on EC 1.2.1.9 - glyceraldehyde-3-phosphate dehydrogenase (NADP+) and Organism(s) Triticum aestivum and UniProt Accession Q8LK61

for references in articles please use BRENDA:EC1.2.1.9
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Triticum aestivum
UNIPROT: Q8LK61 not found.
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Word Map
  • 1.2.1.9
  • groes
  • chaperonins
  • refolding
  • atpase
  • heat-shock
  • co-chaperonins
  • rhodanese
  • groel-groes
  • chloroplast
  • heptameric
  • tubulins
  • double-ring
  • encapsulate
  • misfolded
  • machine
  • malate
  • anti-hsp60
  • equatorial
  • t-complex
  • rubisco
  • cage
  • toroid
  • mg-atp
  • co-chaperone
  • cryo-electron
  • endosymbiotic
  • chlamydial
  • amp-pnp
  • atp-induced
  • aphid
  • endosymbionts
  • molten
  • hetero-oligomeric
  • coarse-grained
  • protein-folding
  • back-to-back
  • nonnative
  • trachomatis
The taxonomic range for the selected organisms is: Triticum aestivum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, triosephosphate dehydrogenase, gap dehydrogenase, apo-gapdh, np-ga3pdhase, apo-glyceraldehyde-3-phosphate dehydrogenase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, np-gapdh, non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase, gapdhn, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
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dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate)
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-
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glyceraldehyde 3-phosphate dehydrogenase (NADP)
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-
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glyceraldehyde phosphate dehydrogenase (NADP)
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-
-
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Glyceraldehyde-3-phosphate dehydrogenase [NADP+]
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-
-
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glyceraldehyde-3-phosphate:NADP reductase
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-
-
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NADP-glyceraldehyde phosphate dehydrogenase
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NADP-glyceraldehyde-3-phosphate dehydrogenase
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-
-
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Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase
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-
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non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
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nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase
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-
-
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NP-Ga3PDHase
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triose phosphate dehydrogenase
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-
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Triosephosphate dehydrogenase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
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oxidation
reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde-3-phosphate:NADP+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-92-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde-3-phosphate + NADP+
D-3-phosphoglycerate + NADPH
show the reaction diagram
-
-
-
?
D-glyceraldehyde 3-phosphate + NADP+ + H2O
3-phospho-D-glycerate + NADPH + H+
show the reaction diagram
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-
-
-
ir
D-glyceraldehyde-3-phosphate + NADP+
D-3-phosphoglycerate + NADPH
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
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phosphorylated enzyme present in heterotrophic cells is activated up to 3fold, no effect with the non-phosphorylated enzyme from leaves. MgCl2 disrupts the interaction between the enzyme and a 14-3-3 regulatory protein
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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after interaction with the 14-3-3 regulatory protein the phosphorylated enzyme exhibits higher sensitivity to inhibition
Diamide
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sensitive to inactivation by diamide, inactivation is protected by D-glyceraldehyde 3-phosphate (with no effect by NADP+) and it is completely reversed by 2-mercaptoethanol
diphosphate
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after interaction with the 14-3-3 regulatory protein the phosphorylated enzyme exhibits higher sensitivity to inhibition
H2O2
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72% residual activity at 1 mM H2O2
Mg2+
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near 75% of the activity is lost when 5 mM Mg2+ is included in the incubation medium
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methyl viologen
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the specific activity of the enzyme increases up to 2fold after oxidative conditions imposed by methylviologen, expression of NP-Ga3PDHase in plants is induced by high treatments with methyl viologen
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.101 - 0.118
D-Glyceraldehyde-3-phosphate
0.034 - 0.06
NADP+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GAPN_WHEAT
496
0
53047
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
240000
native enzyme independent of phosphorylation state, gel filtration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
reversible phosphorylation can regulate NADPH production in the cytosol of heterotrophic plant cells, the phosphorylated enzyme form has similar affinity for substrate but significant lower maximal velocity
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
a relative stability of NP-Ga3PDHase mainly accounts for the increase in specific activity observed in treatments with low and medium levels (up to 0.01 mM) of methyl viologen
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688646
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial purification
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bustos, D.M.; Iglesias, A.A.
Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is post-translationally phosphorylated in heterotrophic cells of wheat (Triticum aestivum)
FEBS Lett.
530
169-173
2002
Triticum aestivum (Q8LK61), Triticum aestivum
Manually annotated by BRENDA team
Bustos, D.M.; Iglesias, A.A.
Phosphorylated non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from heterotrophic cells of wheat interacts with 14-3-3 proteins
Plant Physiol.
133
2081-2088
2003
Triticum aestivum
Manually annotated by BRENDA team
Bustos, D.M.; Bustamante, C.A.; Iglesias, A.A.
Involvement of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase in response to oxidative stress
J. Plant Physiol.
165
456-461
2008
Triticum aestivum, Zea mays
Manually annotated by BRENDA team