Information on EC 1.14.21.1 - (S)-stylopine synthase

for references in articles please use BRENDA:EC1.14.21.1
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.21.1
transferred to EC 1.14.19.64
RECOMMENDED NAME
GeneOntology No.
(S)-stylopine synthase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Isoquinoline alkaloid biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in biosynthesis of stylopine
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-cheilanthifoline + NADPH + H+ + O2
(S)-stylopine + NADP+ + 2 H2O
show the reaction diagram
(S)-scoulerine + NADPH + H+ + O2
nandinine + ?
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-cheilanthifoline + NADPH + H+ + O2
(S)-stylopine + NADP+ + 2 H2O
show the reaction diagram
A0A240FWB4
the enzyme is involved in biosynthesis of stylopine
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?
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different
Manually annotated by BRENDA team
expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coexpression of cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2) in Pichia pastoris. Biosynthetic enzyme expression is examined in a consolidated system with all genes expressed in one cell and a coculture system with three cell lines that each express a single gene were examined. Although both systems efficiently converted reticuline to stylopine, the consolidated system is more rapid and efficient than the co-culture system. However, substrate-feeding experiments reveal a decrease in the conversion efficiency in the consolidated system during successive cultures, whereas the conversion efficiency in the co-culture system remains constant. Thus, the final amount of stylopine produced from reticuline after successive feedings in the co-culture system is more than 150 nmoles from 750 nmoles of (R,S)-reticuline (375 nmoles of (S)-reticuline)
heterologously expressed in Saccharomyces cerevisiae
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells
synthesis
a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells