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Information on EC 1.14.14.57 - taurochenodeoxycholate 6alpha-hydroxylase and Organism(s) Sus scrofa and UniProt Accession Q9GJX5

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IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein. Requires cytochrome b5 for maximal activity. Acts on taurochenodeoxycholate, taurodeoxycholate and less readily on lithocholate and chenodeoxycholate. In adult pig (Sus scrofa), hyocholic acid replaces cholic acid as a primary bile acid .
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This record set is specific for:
Sus scrofa
UNIPROT: Q9GJX5
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Word Map
The taxonomic range for the selected organisms is: Sus scrofa
The enzyme appears in selected viruses and cellular organisms
Synonyms
cyp4a21, taurochenodeoxycholic acid 6alpha-hydroxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
taurochenodeoxycholic acid 6alpha-hydroxylase
-
CYP3A4
-
-
CYP4A21
-
-
taurochenodeoxycholate 6alpha-monooxygenase
-
-
taurochenodeoxycholic acid 6alpha-hydroxylase
-
-
SYSTEMATIC NAME
IUBMB Comments
taurochenodeoxycholate,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (6alpha-hydroxylating)
A cytochrome P-450 (heme-thiolate) protein. Requires cytochrome b5 for maximal activity. Acts on taurochenodeoxycholate, taurodeoxycholate and less readily on lithocholate and chenodeoxycholate. In adult pig (Sus scrofa), hyocholic acid replaces cholic acid as a primary bile acid [5].
CAS REGISTRY NUMBER
COMMENTARY hide
105669-85-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chenodeoxycholate + [reduced NADPH-hemoprotein reductase] + O2
hyocholate + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
?
taurochenodeoxycholate + [reduced NADPH-hemoprotein reductase] + O2
taurohyocholate + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
?
lithocholate + [reduced NADPH-hemoprotein reductase] + O2
hyocholate + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
activity is low compared to activity with taurochenodeoxycholate
-
-
?
taurochenodeoxycholate + [reduced NADPH-hemoprotein reductase] + O2
taurohyocholate + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
-
cytochrome P450
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0629
taurochenodeoxycholate
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CP4AL_PIG
504
2
57216
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
-
x * 53000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 53000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
6alpha-hydroxylase (CYP4A21) gene: evolution by gene duplication and gene conversion
transfection into COS cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lundell, K.
The porcine taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21) gene: evolution by gene duplication and gene conversion
Biochem. J.
378
1053-1058
2004
Sus scrofa (Q9GJX5), Sus scrofa
Manually annotated by BRENDA team
Araya, Z.; Hellman, U.; Hansson, R.
Characterisation of taurochenodeoxycholic acid 6alpha-hydroxylase from pig liver microsomes
Eur. J. Biochem.
231
855-861
1995
Sus scrofa
Manually annotated by BRENDA team
Lundell, K.; Hansson, R.; Wikvall, K.
Cloning and expression of a pig liver taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21): a novel member of the CYP4A subfamily
J. Biol. Chem.
276
9606-9612
2001
Sus scrofa (Q9GJX5), Sus scrofa
Manually annotated by BRENDA team
Lundell, K.; Wikvall, K.
Gene structure of pig sterol 12alpha-hydroxylase (CYP8B1) and expression in fetal liver comparison with expression of taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21)
Biochim. Biophys. Acta
1634
86-96
2003
Sus scrofa (Q9GJX5), Sus scrofa
Manually annotated by BRENDA team