Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.14.14.132 - (-)-4'-demethyl-deoxypodophyllotoxin 4-hydroxylase and Organism(s) Sinopodophyllum hexandrum and UniProt Accession A0A0N7F297

for references in articles please use BRENDA:EC1.14.14.132
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Sinopodophyllum hexandrum. The enzyme produces the direct precursor to etoposide, a potent anticancer drug. It can also act on (-)-deoxypodophyllotoxin with lower efficiency.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Sinopodophyllum hexandrum
UNIPROT: A0A0N7F297
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The taxonomic range for the selected organisms is: Sinopodophyllum hexandrum
The expected taxonomic range for this enzyme is: Sinopodophyllum hexandrum
Synonyms
CYP82D61, EC 1.14.13.214, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CYP82D61
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(–)-deoxypodophyllotoxin,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (4-hydroxylating)
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Sinopodophyllum hexandrum. The enzyme produces the direct precursor to etoposide, a potent anticancer drug. It can also act on (-)-deoxypodophyllotoxin with lower efficiency.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-4'-demethyl-deoxypodophyllotoxin + [reduced NADPH-hemoprotein reductase] + O2
(-)-4'-demethylepipodophyllotoxin + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
or Sinopodophyllum hexandrum, gene CYP82D61
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the biosynthesis of (-)-4'-demethylepipodophyllotoxin (the etoposide aglycone), a naturally occurring lignan that is the immediate precursor of etoposide and, unlike podophyllotoxin, a potent topoisomerase inhibitor. Biosynthetic pathway of (-)-podophyllotoxin, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C82D6_SINHE
519
2
59010
Swiss-Prot
Secretory Pathway (Reliability: 3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene CYP82D61, expression analysis by qRT-PCR analysis, transcriptome mining to identify biosynthetic genes in the podophyllotoxin pathway, Agrobacterium-mediated functional transient expression in Nicotiana benthamiana
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
recombinant coexpression of the genes involved in the podophyllotoxin pathway permits to reconstitute the pathway to (-)-4'-demethylepipodophyllotoxin (the etoposide aglycone), a naturally occurring lignan that is the immediate precursor of etoposide and, unlike podophyllotoxin, a potent topoisomerase inhibitor, for production of the etoposide aglycone in tobacco and circumvent the need for cultivation of mayapple and semisynthetic epimerization and demethylation of podophyllotoxin
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lau, W.; Sattely, E.
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone
Science
349
1224-1228
2015
Sinopodophyllum hexandrum (A0A0N7F297)
Manually annotated by BRENDA team