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Information on EC 1.14.14.109 - 3-hydroxyindolin-2-one monooxygenase and Organism(s) Zea mays and UniProt Accession Q43250

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IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
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This record set is specific for:
Zea mays
UNIPROT: Q43250
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The taxonomic range for the selected organisms is: Zea mays
The enzyme appears in selected viruses and cellular organisms
Synonyms
cyp71c1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cyp71C1
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SYSTEMATIC NAME
IUBMB Comments
3-hydroxyindolin-2-one,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one-forming)
A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxyindolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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-
-
?
3-hydroxyindolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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cytochrome P450
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.031 - 0.036
3-hydroxyindolin-2-one
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
-
recombinant enzyme, at pH 7.5 and 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C71C1_MAIZE
535
0
59716
Swiss-Prot
Secretory Pathway (Reliability: 5)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae WAT11 cells
expressed in Saccharomyces cerevisiae strain WAT11
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expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3)
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CYP71C1 is expressed at a significant and equal level in 2.5 and 6.5 day old seedling shoots, induced 2.1fold in naphthalic anhydride- and naphthalic anhydride/triasulfuron-treated 2.5 day old seedling shoots
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CYP71C1 is repressed in older naphthalic anhydride and naphthalic anhydride/triasulfuron-treated seedling shoots
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CYP71C1 transcript levels in shoots and roots are unaffected by phenobarbital treatment with or without triasulfuron. The pathogens Erwinia stuartii and Acidovorax avenae induce CYP71C3 transcript 1.9 and 2.5fold, respectively
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Frey, M.; Chomet, P.; Glawischnig, E.; Stettner, C.; Grun, S.; Winklmair, A.; Eisenreich, W.; Bacher, A.; Meeley, R.B.; Briggs, S.P.; Simcox, K.; Gierl, A.
Analysis of a chemical plant defense mechanism in grasses
Science
277
696-699
1997
Zea mays (Q43250)
Manually annotated by BRENDA team
Glawischnig, E.; Grn, S.; Frey, M.; Gierl, A.
Cytochrome P450 monooxygenases of DIBOA biosynthesis: Specificity and conservation among grasses
Phytochemistry
50
925-930
1999
Zea mays
Manually annotated by BRENDA team
Spiteller, P.; Glawischnig, E.; Gierl, A.; Steglich, W.
Studies on the biosynthesis of 2-hydroxy-1,4-benzoxazin-3-one (HBOA) from 3-hydroxyindolin-2-one in Zea mays
Phytochemistry
57
373-376
2001
Zea mays
Manually annotated by BRENDA team
Persans, M.; Wang, J.; Schuler, M.
Characterization of maize cytochrome P450 monooxygenases induced in response to safeners and bacterial pathogens
Plant Physiol.
125
1126-1138
2001
Zea mays
Manually annotated by BRENDA team