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Information on EC 1.13.11.56 - 1,2-dihydroxynaphthalene dioxygenase and Organism(s) Sphingobium xenophagum and UniProt Accession P74836

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IUBMB Comments
This enzyme is involved in naphthalene degradation. Requires Fe2+.
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This record set is specific for:
Sphingobium xenophagum
UNIPROT: P74836
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Word Map
The taxonomic range for the selected organisms is: Sphingobium xenophagum
The enzyme appears in selected viruses and cellular organisms
Synonyms
1,2-dihydroxynaphthalene dioxygenase, 1,2-dihydroxynaphthalene oxygenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,2-DHN dioxygenase
-
-
SYSTEMATIC NAME
IUBMB Comments
1,2-dihydroxynaphthalene:oxygen oxidoreductase
This enzyme is involved in naphthalene degradation. Requires Fe2+.
CAS REGISTRY NUMBER
COMMENTARY hide
55354-35-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2,5-trihydroxynaphthalene + O2
?
show the reaction diagram
-
-
-
-
?
1,2,6-trihydroxynaphthalene + O2
?
show the reaction diagram
-
-
-
-
?
1,2,7-trihydroxynaphthalene + O2
?
show the reaction diagram
-
-
-
-
?
1,2-dihydroxynaphthalene + O2
2-hydroxy-2H-chromene-2-carboxylate
show the reaction diagram
-
-
-
-
?
1,2-dihydroxynaphthalene + O2
2-hydroxychromene-2-carboxylate
show the reaction diagram
-
induced in presence of naphthalene-2-sulfonic acid
-
-
?
2,3-dihydroxybiphenyl + O2
?
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybiphenyl + O2
?
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,2-dihydroxynaphthalene + O2
2-hydroxychromene-2-carboxylate
show the reaction diagram
-
induced in presence of naphthalene-2-sulfonic acid
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
required for maximal activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034
1,2-dihydroxynaphthalene
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
BN6
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NSAC_SPHXE
298
0
33301
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
290000
-
gel filtration
33000
-
8 * 33000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
-
8 * 33000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme is more stable at slightly alkaline pH values and better preserved in Na-K phosphate buffer than in Tris-HCI or glycine-NaOH
698539
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme in crude extract is stable in Na-K phosphate buffer, pH 7.5, after 1 week of storage (2.4 mg of protein per ml) at 4°C, 100% of the original activity is recovered
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
-
storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated
Ethanol
-
storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a gene cluster is identified on the plasmid pBN6 which codes for several enzymes participating in the degradative pathway for naphthalenesulfonates. A DNA fragment of 16915 bp is sequenced which contains 17 ORFs. The genes encoding the 1,2-dihydroxynaphthalene dioxygenase, 2-hydroxychromene-2-carboxylate isomerase, and 29-hydroxybenzalpyruvate aldolase of the naphthalenesulfonate pathway are identified on the DNA fragment and the encoded proteins heterologously expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kuhm, A.E.; Stolz, A.; Ngai, K.L.; Knackmuss, H.J.
Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids
J. Bacteriol.
173
3795-3802
1991
Sphingobium xenophagum, Sphingobium xenophagum BN6
Manually annotated by BRENDA team
Keck, A.; Conradt, D.; Mahler, A.; Stolz, A.; Mattes, R.; Klein, J.
Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6
Microbiology
152
1929-1940
2006
Sphingobium xenophagum (P74836), Sphingobium xenophagum, Sphingobium xenophagum BN6 (P74836)
Manually annotated by BRENDA team