Information on EC 1.13.11.17 - indole 2,3-dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.17
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RECOMMENDED NAME
GeneOntology No.
indole 2,3-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
indole + O2 = 2-formylaminobenzaldehyde
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole degradation to anthranil and anthranilate
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Tryptophan metabolism
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SYSTEMATIC NAME
IUBMB Comments
indole:oxygen 2,3-oxidoreductase (decyclizing)
Enzymes from the plants Tecoma stans, Jasminum grandiflorum and Zea mays are flavoproteins containing copper. They are part of enzyme systems that form either anthranil (2,1-benzoisoxazole) (Tecoma stans), anthranilate (Jasminum grandiflorum) or both (Zea mays) as the final product. A second enzyme from Tecoma stans is not a flavoprotein, does not require copper, and is part of a system that forms anthranilate as the final product.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-57-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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increased enzyme expression in plasmablastic lymphoma, a rare and aggressive diffuse large B-cell lymphoma commonly associated with Epstein-Barr virus co-infection that most often occurs in the context of human immunodeficiency virus infection. The enzyme acts as immunosuppressive molecule and is involved in the tumor immune escape mechanism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-bromoindole + O2
5-bromo-2-formylaminobenzaldehyde
show the reaction diagram
5-hydroxyindole + O2
2-formylamino-5-hydroxybenzaldehyde
show the reaction diagram
5-methylindole + O2
2-formylamino-5-methylbenzaldehyde
show the reaction diagram
indole + O2
?
show the reaction diagram
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during daytime activity is less, it reaches a peak during nighttime
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indole + O2
anthranilic acid
show the reaction diagram
o-aminobenzaldehyde + O2
anthranilic acid
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
indole + O2
?
show the reaction diagram
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during daytime activity is less, it reaches a peak during nighttime
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
8-hydroxyquinoline
Atebrin
azide
CO
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0.5 mM, 58% inhibition
Cu2+
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1 mM, CuSO4, 25% inhibition
cyanide
diethyldithiocarbamate
Dithionite
dithiothreitol
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0.0005 mM, 97% inhibition
iodoacetate
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0.0005 mM, 19% inhibition
Neocuproine
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0.001 mM, 92% inhibition
Salicylaldoxime
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0189 - 0.14
indole
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0157
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disappearance of 1 nmol indole in 1 min
0.0441
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disappearance of 1 nmol indole in 1 min
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.8 - 6.2
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pH 3.8: about 80% of maximal activity, pH 5.8: about 65% of maximal activity, pH 6.2: about 30% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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with or without Epstein-Barr virus infection
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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activity is present in relevant amounts even in newborn rats. The specific activity increases significantly in young rats by about 44% in comparison to newborns
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
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gel filtration
98000
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 6
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stable
395575
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis results in complete loss of activity
dialysis, 8 h, results in complete loss of activity
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pH 7, 0.02 M sodium phosphate buffer, 0.1 mM EDTA, 6 h in the cold, slight loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 18 h without appreciable loss of activity, complete loss of activity after 48 h
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-20°C, 20 d, no appreciable loss of activity
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4°C, stable for 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
increased enzyme expression via expression of CD163 antigen in plasmablastic lymphoma, a rare and aggressive diffuse large B-cell lymphoma commonly associated with Epstein-Barr virus co-infection that most often occurs in the context of human immunodeficiency virus infection
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