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Information on EC 1.13.11.14 - 2,3-dihydroxybenzoate 3,4-dioxygenase
for references in articles please use BRENDA:EC1.13.11.14
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EC Tree 1 Oxidoreductases
1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
1.13.11 With incorporation of two atoms of oxygen
1.13.11.14 2,3-dihydroxybenzoate 3,4-dioxygenase
The enzyme appears in viruses and cellular organisms
showSynonyms
2,3-dihydroxybenzoate 3,4-dioxygenase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2,3-dihydroxybenzoate 1,2-dioxygenase
-
-
-
-
2,3-dihydroxybenzoate oxygenase
-
-
-
-
2,3-dihydroxybenzoic oxygenase
-
-
-
-
o-pyrocatechuate oxygenase
-
-
-
-
oxygenase, 2,3-dihydroxybenzoate 3,4-di-
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2,3-dihydroxybenzoate + O2 = 3-carboxy-2-hydroxymuconate semialdehyde
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decarboxylation
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
2,3-dihydroxybenzoate degradation
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SYSTEMATIC NAME
IUBMB Comments
2,3-dihydroxybenzoate:oxygen 3,4-oxidoreductase (decyclizing)
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CAS REGISTRY NUMBER
COMMENTARY
9032-31-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxybenzoate + O2
3-carboxy-2-hydroxymuconate semialdehyde
Substrates: -
Products: -
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2,3-dihydroxy-p-toluate + O2
2,6-dioxo-4-heptenoic acid + CO2
-
Substrates: -
Products: -
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?
2,3-dihydroxy-p-toluate + O2
2,6-dioxo-4-heptenoic acid + CO2
-
Substrates: -
Products: -
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2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: -
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: cleavage at 3,4-position
Products: -
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?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: -
Products: -
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?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: cleavage at 3,4-position
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: -
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: cleavage at 3,4-position
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: -
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: -
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: cleavage at 3,4-position
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: cleavage at 3,4-position
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?
additional information
?
-
-
Substrates: no substrate: 3-methylcatechol
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additional information
?
-
-
Substrates: no substrate: 3-methylcatechol
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additional information
?
-
-
Substrates: no substrates: itoic acid, catechol and protocatechuic acid
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: -
Products: -
Products: -
?
2,3-dihydroxybenzoate + O2
CO2 + alpha-hydroxymuconic semialdehyde
-
Substrates: -
Products: -
Products: -
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.261
-
specific activity in cell-free extract after growth on 2,3-dihydroxybenzoate
0.508
-
specific activity in cell-free extract after growth on 2-sulfobenzoate
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
enzyme is encoded by OpaC in a gene cluster involved in the o-phthalate catabolism. The opa gene products, opaAaAbAcAd, opaB, opaC, and opaD code for o-phthalate 2,3-dioxygenase, dihydrodiol dehydrogenase, 2,3-dihydroxybenzoate 3,4-dioxygenase, and 3-carboxy-2-hydroxymuconate-6-semialdehyde decarboxylase, respectively. Their products are required for the utilization of o-phthalate
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A840Q943_9PSEU
302
0
33007
TrEMBL
-
A0A0P0RMJ4_9BURK
287
0
32533
TrEMBL
-
A0A7Y9EVD2_9MICO
302
0
34193
TrEMBL
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ito, T.
Enzymic determination of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid
Appl. Environ. Microbiol.
59
2343-2345
1993
Pseudomonas fluorescens, Pseudomonas putida
brenda
Rettenmaier, H.; Lingens, F.
Purification and some properties of two isofunctional juglone hydroxylases from Pseudomonas putida J1
Biol. Chem. Hoppe-Seyler
366
637-646
1985
Pseudomonas putida, Pseudomonas putida J1
brenda
Ribbons, D.W.
Bacterial oxidation of 2,3-dihydroxybenzoic acid - a new oxygenase
Biochem. J.
99
30P-31P
1966
Pseudomonas fluorescens, Pseudomonas fluorescens 23D-1
-
brenda
Ribbons, D.W.; Senior, P.J.
2,3-Dihydroxybenzoate 3,4-oxygenase from Pseudomonas fluorescens: determination of the site of ring cleavage with a substrate analogue
Biochem. J.
117
28P-29P
1970
Pseudomonas fluorescens, Pseudomonas fluorescens 23D-1
brenda
Ribbons, D.W.; Senior, J.J.
2,3-Dihydroxybenzoate 3,4-oxygenase from Pseudomonas fluorescens: oxidation of a substrate analog
Arch. Biochem. Biophys.
138
557-565
1970
Pseudomonas fluorescens, Pseudomonas fluorescens 23D-1
brenda
Rettenmaier, H.; Kupas, U.; Lingens, F.
Degradation of juglone by Pseudomonas putida J1
FEMS Microbiol. Lett.
19
193-195
1983
Pseudomonas putida, Pseudomonas putida J1
-
brenda
Hansen, C.; Fortnagel, P.; Wittich, R.M.
Initial reactions in the mineralization of 2-sulfobenzoate by Pseudomonas sp. RW611
FEMS Microbiol. Lett.
92
35-40
1992
Pseudomonas sp., Pseudomonas sp. RW611
-
brenda
Kasai, D.; Iwasaki, T.; Nagai, K.; Araki, N.; Nishi, T.; Fukuda, M.
2,3-Dihydroxybenzoate meta-cleavage pathway is involved in o-phthalate utilization in Pseudomonas sp. strain PTH10
Sci. Rep.
9
1253
2019
Pseudomonas sp. (A0A2Z5UC95), Pseudomonas sp.
brenda
EXTERNAL LINKS (specific for EC-Number 1.13.11.14)
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Release 2023.1 (January 2023)
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