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Information on EC 1.10.3.3 - L-ascorbate oxidase and Organism(s) Nicotiana tabacum and UniProt Accession Q40588

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IUBMB Comments
A multicopper protein.
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This record set is specific for:
Nicotiana tabacum
UNIPROT: Q40588
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The taxonomic range for the selected organisms is: Nicotiana tabacum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
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4
L-ascorbate
+
O2
=
4
monodehydroascorbate
+
2
H2O
4
+
=
4
+
2
Synonyms
ascorbate oxidase, ascorbic acid oxidase, l-ascorbate oxidase, aa-ox, af_ao1, aa oxidase, ascorbic oxidase, asc oxidase, l-ascorbic acid oxidase, l-ascorbate:oxygen oxidoreductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AA oxidase
-
-
-
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AAO
-
-
-
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ascorbase
-
-
-
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ascorbate dehydrogenase
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-
-
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ascorbate oxidase
ascorbic acid oxidase
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-
-
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ascorbic oxidase
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-
-
-
L-ascorbate:O2 oxidoreductase
-
-
-
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L-ascorbic acid oxidase
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-
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oxidase, ascorbate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
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oxidation
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reduction
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-
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-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
L-ascorbate:oxygen oxidoreductase
A multicopper protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-44-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 L-ascorbic acid + O2
2 L-dehydroascorbic acid + 2 H2O
show the reaction diagram
-
-
-
-
?
additional information
?
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-
cell wall-localized enzyme that catalyses the oxidation of ascorbate to the unstable radical monodehydroascorbate, which rapidly disproportionates to yield dehydroascorbate and ascorbate, and thus contributes to the regulation of the ascorbic acid redox state regulating the cell redox status
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 L-ascorbic acid + O2
2 L-dehydroascorbic acid + 2 H2O
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
cell wall-localized enzyme that catalyses the oxidation of ascorbate to the unstable radical monodehydroascorbate, which rapidly disproportionates to yield dehydroascorbate and ascorbate, and thus contributes to the regulation of the ascorbic acid redox state regulating the cell redox status
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
induction of enzyme by light or auxin, salicylic acid inhibits
Swissprot
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ASO_TOBAC
578
0
64865
Swiss-Prot
Secretory Pathway (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
construction of three transgenic enzyme overexpressing lines R50, R60, and R68, selected on the basis of enzyme expression and single transgene copy number, the overexpression has a slight effect on chlorophyll and carotinoid contents, and on sensitivity to oxidative stress, phenotypes, overview
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
comparison of tolerance of wild-type and transgenic tobacco plants to oxidative stress, overview
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675145
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of the enzyme in transgenic tobacco lines
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
expression of enzyme in sense- and antisense-orientations, enhanced enzyme activity oxidizes the apoplastic ascorbate pool, decreased enzyme activity increases the amount of ascorbate compared with dehydroascorbate. In sense and antisense plants, enzyme transcript levels are no longer subject to light/dark regulation. Relationship between enzyme activity and plant height and biomass
agriculture
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expression of enzyme gene in sense and antisense orientation, no significant differences in phenotype except for a delay in flowering time in antisense palnts. At high salinity, increase in percentage of germination, photosynthetic activity and seed yield in antisense plants. Sense plants show a very low redox state of apoplastic ascorbate and increased hydrogen peroxide contents in symplastic and apoplastic spaces
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamamoto, A.; Bhuiyan, M.N.; Waditee, R.; Tanaka, Y.; Esaka, M.; Oba, K.; Jagendorf, A.T.; Takabe, T.
Suppressed expression of the apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants
J. Exp. Bot.
56
1785-1796
2005
Arabidopsis thaliana, Nicotiana tabacum
Manually annotated by BRENDA team
Pignocchi, C.; Fletcher, J.M.; Wilkinson, J.E.; Barnes, J.D.; Foyer, C.H.
The function of ascorbate oxidase in tobacco
Plant Physiol.
132
1631-1641
2003
Nicotiana tabacum (Q40588), Nicotiana tabacum
Manually annotated by BRENDA team
Fotopoulos, V.; Sanmartin, M.; Kanellis, A.K.
Effect of ascorbate oxidase over-expression on ascorbate recycling gene expression in response to agents imposing oxidative stress
J. Exp. Bot.
57
3933-3943
2006
Nicotiana tabacum
Manually annotated by BRENDA team