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Information on EC 1.1.1.392 - 3alpha-hydroxycholanate dehydrogenase (NADP+) and Organism(s) Eggerthella lenta and UniProt Accession C8WMP0

for references in articles please use BRENDA:EC1.1.1.392
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IUBMB Comments
This bacterial enzyme is involved in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids) via a 3-oxo intermediate. The enzyme catalyses a reversible reaction in vitro. Also acts on related bile acids. cf. EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+).
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This record set is specific for:
Eggerthella lenta
UNIPROT: C8WMP0
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Word Map
The taxonomic range for the selected organisms is: Eggerthella lenta
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
3alpha-HSDH, alpha-hydroxy-cholanate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
lithocholate:NADP+ 3-oxidoreductase
This bacterial enzyme is involved in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids) via a 3-oxo intermediate. The enzyme catalyses a reversible reaction in vitro. Also acts on related bile acids. cf. EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1280
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
pH 7.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.85
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
pH 7.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene Elen_0690
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids
physiological function
the iso–bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Elen_0690, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Devlin, A.; Fischbach, M.
A biosynthetic pathway for a prominent class of microbiota-derived bile acids
Nat. Chem. Biol.
11
685-690
2015
Eggerthella lenta (C8WMP0), Eggerthella lenta ATCC 25559 (C8WMP0)
Manually annotated by BRENDA team