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Information on EC 1.1.1.183 - geraniol dehydrogenase (NADP+) and Organism(s) Ocimum basilicum and UniProt Accession Q2KNL6

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EC Tree
IUBMB Comments
Also acts, more slowly on farnesol but not on nerol. The enzyme produces a mixture known as citral, which includes geranial and neral. It is still not known whether neral is produced directly by the enzyme, or by isomerization of geranial.
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This record set is specific for:
Ocimum basilicum
UNIPROT: Q2KNL6
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Word Map
The taxonomic range for the selected organisms is: Ocimum basilicum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
geraniol dehydrogenase, gedh1, geraniol-dh i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
geraniol:NADP+ oxidoreductase
Also acts, more slowly on farnesol but not on nerol. The enzyme produces a mixture known as citral, which includes geranial and neral. It is still not known whether neral is produced directly by the enzyme, or by isomerization of geranial.
CAS REGISTRY NUMBER
COMMENTARY hide
56802-96-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-methyl-2-buten-1-ol + NADP+
3-methyl-2-butenal + NADPH
show the reaction diagram
22.7% of the activity with geraniol
-
-
?
3-methyl-3-buten-1-ol + NADP+
3-methyl-3-butenal + NADPH
show the reaction diagram
6.5% of the activity with geraniol
-
-
?
benzylalcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
5.5% of the activity with geraniol
-
-
?
cinnamyl alcohol + NADP+
cinnamyl aldehyde + NADPH
show the reaction diagram
53.0% of the activity with geraniol
-
-
?
cis-3-hexenol + NADP+
cis-3-hexenal + NADPH
show the reaction diagram
13.3% of the activity with geraniol
-
-
?
citronellol + NADP+
citronellal + NADPH
show the reaction diagram
16.6% of the activity with geraniol
-
-
r
geraniol + NADP+
geranial + NADPH
show the reaction diagram
hexanol + NADP+
hexanal + NADPH
show the reaction diagram
31.6% of the activity with geraniol
-
-
?
nerol + NADP+
neral + NADPH + H+
show the reaction diagram
phenylethanol + NADP+
phenylethanal + NADPH
show the reaction diagram
10.2% of the activity with geraniol
-
-
?
phenylpropanol + NADP+
phenylpropanal + NADPH
show the reaction diagram
22.1% of the activity with geraniol
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geraniol + NADP+
geranial + NADPH
show the reaction diagram
the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview
-
-
ir
nerol + NADP+
neral + NADPH + H+
show the reaction diagram
-
-
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Zn2+
66% inhibition at 5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
geraniol
pH 9.5
0.037
nerol
pH 9.5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 10
over 72% of maximal activity at pH 8.5 and pH 10.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GEDH1; cv. Sweet Dani, gene GEDH1
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest activity in young leaves decreasing to 60% in older leaves
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GEDH1_OCIBA
360
0
39044
Swiss-Prot
other Location (Reliability: 5)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GEDH1 from Escherichia coli by anion exchange chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
GEDH1, phylogenetic tree and analysis, sequence comparison, expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Iijima, Y.; Wang, G.; Fridman, E.; Pichersky, E.
Analysis of the enzymatic formation of citral in the glands of sweet basil
Arch. Biochem. Biophys.
448
141-149
2006
Ocimum basilicum (Q2KNL6), Ocimum basilicum
Manually annotated by BRENDA team