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Information on EC 1.1.1.151 - 21-hydroxysteroid dehydrogenase (NADP+)

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EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.151 21-hydroxysteroid dehydrogenase (NADP+)

IUBMB Comments

Acts on a number of 21-hydroxycorticosteroids.

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The enzyme appears in viruses and cellular organisms

Synonyms

21-hydroxy steroid (NADP) dehydrogenase, 21-hydroxy steroid dehydrogenase, 21-hydroxy steroid dehydrogenase (NADP), NADP-21-hydroxysteroid dehydrogenase, show all synonyms

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

21-hydroxy steroid (NADP) dehydrogenase

21-hydroxy steroid dehydrogenase

21-hydroxy steroid dehydrogenase (NADP)

NADP-21-hydroxysteroid dehydrogenase

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

pregnan-21-ol + NADP+ = pregnan-21-al + NADPH + H+

Acts on a number of 21-hydroxycorticosteroids

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

oxidation

redox reaction

reduction

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SYSTEMATIC NAME

IUBMB Comments

21-hydroxysteroid:NADP+ 21-oxidoreductase

Acts on a number of 21-hydroxycorticosteroids.

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CAS REGISTRY NUMBER

COMMENTARY

37250-76-3

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADPH

11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NADP+

Ovis aries aries

i.e. 21-dehydro-delta1-cortisol

286004

21-hydroxy-delta1-cortisol

286004

11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADPH

11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+

Ovis aries aries

i.e. 21-dehydrocortisol

286004

i.e. cortisol

286004

11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADPH

11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+

Ovis aries aries

i.e. 21-dehydrocorticosterone

286004

21-hydroxycorticosterone

286004

17-hydroxy-pregn-4-ene-3,20,21-trione + NADPH

17,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+

Ovis aries aries

i.e. 21-dehydro-11-deoxycortisol

286004

i.e. 21-hydroxy-11-deoxycortisol

286004

17alpha-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH

17alpha,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+

Ovis aries aries

i.e. 21-dehydrocortisone, marked dependence on the nature of steroids

286004

i.e. cortisone

286004

pregn-4-ene-3,20,21-trione + NADPH

21-hydroxy-pregn-4-ene-3,20-dione + NADP+

Ovis aries aries

i.e. 21-dehydro-11-deoxy-corticosterone

286004

21-hydroxy-11-deoxy-corticosterone

286004

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NADPH

Ovis aries aries

286004

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

Ag+

Ovis aries aries

complete inhibition at 0.004 mM

286004

Hg2+

Ovis aries aries

53% inhibition at 0.002 mM

286004

NADH

Ovis aries aries

competitive inhibition

286004

NADPH

Ovis aries aries

competitive inhibition

286004

p-mercuribenzoate

Ovis aries aries

55% inhibition at 0.014 mM

286004

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.137

11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione

Ovis aries aries

286004

0.177

11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione

Ovis aries aries

286004

0.233

11beta-hydroxy-pregn-4-ene-3,20,21-trione

Ovis aries aries

286004

0.461

17-hydroxy-pregn-4-ene-3,11,20,21-tetraone

Ovis aries aries

286004

0.137

17-hydroxy-pregn-4-ene-3,20,21-trione

Ovis aries aries

286004

0.021

NADPH

Ovis aries aries

286004

0.118

pregn-4-ene-3,20,21-trione

Ovis aries aries

286004

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5.9 - 6.1

Ovis aries aries

286004

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Ovis aries aries

sheep

286004

brenda

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

liver

Ovis aries aries

286004

brenda

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ovis aries aries

80% loss of activity in 5 min

286004

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

not sensitive against repeated freezing and thawing

Ovis aries aries

286004

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, 0.01 M sodium phosphate , pH 6-7.4, 1 year

Ovis aries aries

286004

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PURIFICATION/commentary

ORGANISM

UNIPROT

LITERATURE

to homogeneity, Sephadex G-100, starch block elelctrophoresis

Ovis aries aries

286004

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

286004

Monder, C.; White, A.

The 21-hydroxysteroid dehydrogenases of liver. A nicotinamide adenine dinucleotide phosphate dehydrogenase and two nicotinamide adenine dinucleotide dehydrogenases

J. Biol. Chem.

240

71-77

1965

Ovis aries aries

14253469

brenda