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Sequence of NHAA_RHOER

EC Number:4.2.1.84

EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
nitrile hydratase
P13448
Rhodococcus erythropolis
207
22996
Reaction
an aliphatic amide = a nitrile + H2O
Other sequences found for EC No. 4.2.1.84

General information:

Sequence
show sequence in fasta format
  0 MSVTIDHTTE NAAPAQAPVS DRAWALFRAL DGKGLVPDGY VEGWKKTFEE DFSPRRGAEL
 60 VARAWTDPEF RQLLLTDGTA AVAQYGYLGP QGEYIVAVED TPTLKNVIVC SLCSCTAWPI
120 LGLPPTWYKS FEYRARVVRE PRKVLSEMGT EIASDIEIRV YDTTAETRYM VLPQRPAGTE
180 GWSQEQLQEI VTKDCLIGVA IPQVPTV
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
703520
Ikehata O.,Nishiyama M.,Horinouchi S.,Beppu T.
Primary structure of nitrile hydratase deduced from the nucleotide sequence of a Rhodococcus species and its expression in Escherichia coli.
Eur. J. Biochem.
181
563-570
1989
703521
Hashimoto Y.,Nishiyama M.,Ikehata O.,Horinouchi S.,Beppu T.
Cloning and characterization of an amidase gene from Rhodococcus species N-774 and its expression in Escherichia coli.
Biochim. Biophys. Acta
1088
225-233
1991
703523
Nojiri M.,Yohda M.,Odaka M.,Matsushita Y.,Tsujimura M.,Yoshida T.,Dohmae N.,Takio K.,Endo I.
Functional expression of nitrile hydratase in Escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a ligand cysteine.
J. Biochem.
125
696-704
1999
703524
Mayaux J.-F.,Cerbelaud E.,Soubrier F.,Faucher D.,Petre D.
Purification, cloning, and primary structure of an enantiomer-selective amidase from Brevibacterium sp. strain R312: structural evidence for genetic coupling with nitrile hydratase.
J. Bacteriol.
172
6764-6773
1990
703525
Endo T.,Watanabe I.
Nitrile hydratase of Rhodococcus sp. N-774. Purification and amino acid sequences.
FEBS Lett.
243
61-64
1989
703526
Huang W.,Jia J.,Cummings J.,Nelson M.,Schneider G.,Lindqvist Y.
Crystal structure of nitrile hydratase reveals a novel iron centre in a novel fold.
Structure
5
691-699
1997
703527
Nagashima S.,Nakasako M.,Dohmae N.,Tsujimura M.,Takio K.,Odaka M.,Yohda M.,Kamiya N.,Endo I.
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms.
Nat. Struct. Biol.
5
347-351
1998
703528
Tsujimura M.,Dohmae N.,Odaka M.,Chijimatsu M.,Takio K.,Yohda M.,Hoshino M.,Nagashima S.,Endo I.
Structure of the photoreactive iron center of the nitrile hydratase from Rhodococcus sp. N-771. Evidence of a novel post-translational modification in the cysteine ligand.
J. Biol. Chem.
272
29454-29459
1997