EC Number   |
Protein Variants |
Reference |
|---|
    6.3.2.3 | A485L/T486P |
site-directed mutagenesis, the mutant shows 70% reduced activity compared to the wild-type activity, and a shift of substrate specificity with increased affinity of GSH2 for Ser as a substrate, while affinity to Gly is preserved. This provides another biosynthetic pathway for hydroxymethyl glutathione, which is known to be synthesized from glutathione and Ser in a reaction catalysed by carboxypeptidase Y |
728585 |
| 6.3.2.3 | C294A |
mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422 |
1211 |
| 6.3.2.3 | C409A |
mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422 |
1211 |
| 6.3.2.3 | C522A |
mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422 |
1211 |
| 6.3.2.3 | D219A |
naturally occurring missense mutation expressed using a His-tagged, Escherichia coli-based expression system, decreases Vmax to 4% of the wild-type activity |
660972 |
| 6.3.2.3 | D219G |
naturally occurring missense mutation expressed using a His-tagged, Escherichia coli-based expression system, decreases Vmax to 27% of the wild-type activity. Negative cooperativity for L-gamma-glutamyl-L-alpha-aminobutyric acid is changed to positive |
660972 |
| 6.3.2.3 | D24A |
site-directed mutagenesis, the mutant shows a slight increase in catalytic efficiency compared to wild-type enzyme |
746387 |
| 6.3.2.3 | D448A |
low activity |
672053 |
| 6.3.2.3 | D458A |
site-directed mutagenesis, the mutant shows 10% activity compared to the wild-type enzyme |
726858 |
| 6.3.2.3 | D458N |
site-directed mutagenesis, the mutant shows 15% activity compared to the wild-type enzyme |
726858 |
