Cloned (Comment) | Organism |
---|---|
- |
Streptococcus agalactiae |
Protein Variants | Comment | Organism |
---|---|---|
D448A | low activity | Streptococcus agalactiae |
H144A | higher activity than the wild type enzyme | Streptococcus agalactiae |
K485A | very low activity | Streptococcus agalactiae |
K489A | low activity | Streptococcus agalactiae |
K526A | very low activity | Streptococcus agalactiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by glutathione | Streptococcus agalactiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
ATP | wild type enzyme | Streptococcus agalactiae | |
0.624 | - |
ATP | mutant enzyme K526A | Streptococcus agalactiae | |
4.9 | - |
ATP | mutant enzyme D448A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
5.9 | - |
gamma-Glu-L-Cys | wild type enzyme, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
6.3 | - |
Gly | wild type enzyme, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
7.5 | - |
gamma-Glu-L-Cys | mutant enzyme D448A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
7.5 | - |
gamma-Glu-L-Cys | mutant enzyme K526A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
11.8 | - |
Gly | mutant enzyme K526A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
13.4 | - |
ATP | mutant enzyme K489A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
23.8 | - |
Gly | mutant enzyme D448A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
28 | - |
Gly | mutant enzyme K489A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae | |
31 | - |
gamma-Glu-L-Cys | mutant enzyme K489A, in 150 mM Tris-HCl buffer, pH 8.4, 100 mM KCl, 40 mM MgCl2, 0.3 mM EDTA | Streptococcus agalactiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
2 * 88000, gel filtration | Streptococcus agalactiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus agalactiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA resin chromatography | Streptococcus agalactiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-Glu-L-Cys + Gly | - |
Streptococcus agalactiae | ADP + phosphate + glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 88000, gel filtration | Streptococcus agalactiae |
Synonyms | Comment | Organism |
---|---|---|
gamma-GCS-GS | bifunctional enzyme, with a glutathione synthetase at the C terminus | Streptococcus agalactiae |
gamma-glutamylcysteine synthetase-glutathione synthetase | - |
Streptococcus agalactiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptococcus agalactiae |