EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.26 | C255T |
site-directed mutagenesis, mutant substrate specificity and kinetics in comparison to the wild-type enzyme |
683962 |
| 3.4.21.26 | D35A/K196A |
reduced specificity for shorter peptides |
707485 |
| 3.4.21.26 | D641A |
the ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-beta-naphthylamide is 0.2% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 is 1.8fold higher than the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-p-nitrophenol is 66% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-SBzl is 0.92% of the wild-type ratio |
652332 |
| 3.4.21.26 | D641N |
the ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-beta-naphthylamide is 0.02% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate 2-aminobenzoyl-Gly-Phe-Gly-Pro-Phe-Gly-Phe(NO2)-Ala-NH2 is 1.4fold higher than the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-p-nitrophenol is 40% of the wild-type ratio. The ratio of turnover number to Km-value for the substrate benzyloxycarbonyl-Gly-Pro-SBzl is 0.27% of the wild-type ratio |
652332 |
| 3.4.21.26 | E289G |
improved protein stability, 4fold longer half-life at 37°C compared to wild-type |
667606 |
| 3.4.21.26 | F227Y |
mutant of SC PEP |
694952 |
| 3.4.21.26 | F459Y |
mutant of SC PEP |
694952 |
| 3.4.21.26 | I371V |
mutant of SC PEP |
694952 |
| 3.4.21.26 | I406V |
one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions |
694952 |
| 3.4.21.26 | I514V |
one of the mutations that shows the most significant correlation to improved activity and/or stability under simulated gastric conditions |
694952 |
