EC Number |
Protein Variants |
Reference |
---|
3.2.1.75 | C393S |
the mutant shows 0.005% activity compared to the wild type enzyme |
-, 751089 |
3.2.1.75 | C396S |
the mutant shows 0.03% activity compared to the wild type enzyme |
751089 |
3.2.1.75 | D286A |
the mutant shows 0.27% activity compared to the wild type enzyme |
-, 751089 |
3.2.1.75 | E328A |
inactive |
-, 751089 |
3.2.1.75 | E339A |
inactive |
751089 |
3.2.1.75 | E339Q |
inactive |
751089 |
3.2.1.75 | food industry |
the enzyme can be used to hydrolyze beta-1,6-glucans of brown algae or fungal cell walls for producing gentiobiose as a high-value sugar and glucose as a fermentable sugar |
749687 |
3.2.1.75 | H281A |
the mutant shows 0.006% activity compared to the wild type enzyme |
-, 751089 |
3.2.1.75 | more |
gene disruption mutant, shows reduced virulence against Agaricus bisporus and diminished ability to use chitin as a carbon source |
655667 |
3.2.1.75 | more |
the 1,6-beta-D-glucanase gene (Neg1) is disrupted by repeat-induced point mutations. 9 nucleotides within the coding region of the gene change from GC to AT. No apparent phenotypic changes are observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide, which affect fungal cell walls or membranes, markedly inhibit the hyphal growth of the mutant at a concentration that does not inhibi growth of the wild-type |
678778 |