EC Number   |
Protein Variants |
Reference |
|---|
    3.1.1.1 | A179K |
increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 64% enantioneric excess (compared to wild-type value 21% enantiomeric excess) |
-, 726507 |
| 3.1.1.1 | A179M/198E |
increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 18% enantioneric excess (compared to wild-type value 21% enantiomeric excess) |
-, 726507 |
| 3.1.1.1 | A179M/198Y |
increased stereoselectivity in synthesis of naproxen compared to the wild-type enzyme, (S)-selective, 31% enantioneric excess (compared to wild-type value 21% enantiomeric excess) |
-, 726507 |
| 3.1.1.1 | A241N/Q249P/G250E/F276S |
kcat and kcat/Km values for 4-nitrophenyl butyrate hydrolysis are significantly lower than the values of the wild-type enzyme. The mutant shows activations by heat treatment at either 40°C or 50°C |
-, 730054 |
| 3.1.1.1 | A270S |
site-directed mutagenesis, recombinant mutant shows increased activity compared to the recombinant wild-type |
752090 |
| 3.1.1.1 | A400I |
activity with 4-nitrophenol is similar to the wild-type enzyme, similar enantioselectivity in the conversion of the model substrates compared to the wild-type |
650855 |
| 3.1.1.1 | A400I/G105A |
inactive mutant |
650855 |
| 3.1.1.1 | C408A |
kinetics similar to wild-type |
681423 |
| 3.1.1.1 | C408G |
4fold decrease in kcat value |
681423 |
| 3.1.1.1 | C408P |
kinetics similar to wild-type |
681423 |
